Dilek MEMİŞ, Ergün TEMİZ, Beyhan KARAMANLIOĞLU, Halnur ERKÖSE, Zafer PAMUKÇU, Kemal KUTLU, Nesrin TURAN

Ratlarda intraabdominal sepsis modelinde selenyum uygulanması

Çalışmamızda; ratlarda çekal ligasyon ve ponksiyon yöntemiyle (ÇLP) oluşturulan deneysel sepsis modelinde, selenyum uygulanmasının serum nötrofil değerleri ve mortalite üzerine etkisinin, ayrıca akciğerlerin semi-kantitatif histopatolojik incelemesinin değerlendirilmesini amaçladık Çalışma için gerekli 45 adet herbiri 220-235 gr ağırlığında tümü erkek Wistar albino türü rat rasgele olarak 3 gruba ayrıldı. Rasgele olarak gruplardan seçilen toplam 7 rata ketamin 50 mg.kg-1 ım verilerek kontrol nötrofil sayısını saptamak amacıyla kardiyak ponksiyon aracılığı ile kan alındı. Ertesi gün, kalan ratlara septik peritonit oluşturmak amacı ile ÇLP yöntemi uygulandı ve ratlar 3 gruba ayrıldı; Sepsis grubu (Grup I, n=12): ÇLP'yi izleyen 7. günde kan ve doku örnekleri alındı. Seftriakson grubu (Grup II, n=11): ÇLP yapıldıktan 1 saat sonra seftriakson 50 mg.kg-1 im tek doz yapıldı. Ratlara, 7 gün süreyle her gün seftriakson 50 mg kg-1 gün-1 im uygulandı. 7. günde kan ve doku örnekleri alındı. Seftriakson+selenyum grubu (Grup III, n=12): ÇLP yapıldıktan 1 saat sonra seftriakson 50 mg.kg-1 + 100 µg selenyum selanit im tek doz yapıldı. Ratlara, 7 gün süreyle her gün seftriakson 50 mg.kg-1 + 100 µgr.gün-1 selenyum selanit im uygulandı. 7. günde kan ve doku örnekleri alındı. Tüm gruplarda nötrofil sayısı değerlendirildiğinde her üç grupta kontrol grubuna göre ileri derecede artma saptandı. Gruplar kendi aralarında değerlendirildiğinde kan nötrofil sayısında Grup II ve Grup III'deki artış, Grup I'e göre istatistiksel olarak anlamlı derecede daha az (p

Anahtar Kelimeler:

Nötrofiller, Sepsis, Selenyum, Akciğer, Sıçan, Wistar, Hastalık modelleri, hayvan

Selenium application to rats with intraabdominal sepsis model

In our study, our objective was to evaluate the effects of selenium administration on serum neutrophil levels and on mortality and to evaluate the semi-quantitative histopathological examination of the lungs in a rat experimental sepsis model induced by the method of cecal ligation and puncture (CLP). Fourty-five Wistar albino rats weighing 220 to 235 g each were randomized to 3 study groups. A total of 7 rats randomly chosen from the groups were administered 50 mg.kg-1 ım ketamine and blood samples were collected by cardiac puncture to establish control neutrophil values. The following day, the CLP method was applied to induce septic peritonitis. Rats were randomized to the following groups: The sepsis group (Group I, n= 12): blood and tissue samples were collected on the 7th day following CLP. The ceftriaxone group (Group II, n= 11): A single IM dose of ceftriaxone 50 mg.kg-1 ım was administered 1 hour after CLP. Then, a daily ceftriaxone dose of 50 mg.kg-1 ım was maintained for 7 days. Blood and tissue samples were collected on the 7th day. The ceftriaxone plus selenium group (Group III, n= 12): a single dose of ceftriaxone 50 mg.kg-1 ım plus selenium selenide 100 µg im was administered 1 hour after CLP. Then, a daily dose of ceftriaxone 50 mg.kg-1 ım plus selenium selenide 100 µg im was maintained for 7 days. Blood and tissue samples were collected on the 7th day. When neutrophil counts were considered in all groups, a substantial increase was found in all three groups as compared with the control group. When evaluations were made between the groups, the increase in blood neutrophil count was statistically significantly lower in Group II and Group III than that in Goup I (p<0.05) and when Group II and Group III were compared, the increase in blood neutrophil count was found to be statistically significantly lower in Group III (p<0.05). No statistically significant differences were observed between the groups in terms of lung neutrophil count, alveolar-interstitial edema, and alveolar hermorrhage.When mortality rates were evaluated at the end of day 7, Group III receiving ceftriaxone and selenium in combination was found to have lower mortality rates than both of the other groups. In conclusion, we think that in cases where sepsis has developed, the use of selenium reduces mortality, favorably affects blood neutrophil count.

Keywords:

Neutrophils, Sepsis, Selenium, Lung, Rats, Wistar, Disease Models, Animal,

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