Ceren ERDOĞAN, Aysen ERDEM, Seda B. AKINCI, Neslihan Dikmenoğlu, Elif BAŞGÜL, Dicle Balkancı, Ülkü AYPAR

Midazolamın sıçanlarda eritrosit deformabilitesi ve plazma viskozitesi üzerine etkisi

Eritrosit deformabilitesi dokulara oksijen taşınmasında kilit rol oynayan önemli bir fizyolojik faktördür. Plazma viskozitesi ise kan akımına karşı direncin esas belirleyicilerindendir. Ancak, günümüzde sedasyon, indüksiyon ve idamede sıklıkla yeğlenen bir anestetik olan midazolamın eritrosit deformabilitesi ve plazma viskozitesi üzerine etkisini araştıran bir çalışmaya ulaşılamamıştır. Bu araştırmada, midazolamın rotlarda eritrosit deformabilitesi ve plazma viskozitesi üzerine etkilerinin araştırılması amaçlanmıştır. Etik komite izni alındıktan sonra, 20 adet 250-300 gr Wistar albino erkek sıçan rasgele 3 gruba ayrıldı. Grup I: Kontrol gru¬buna hiçbir enjeksiyon yapılmadı (n=8). Grup II: Taşıyıcı grubuna midazolamın ticari formülasyonundaki yardımcı maddelerden oluşan karışımdan, 50 mg kg'1 doza karşılık gelen miktarda intraperitoneal (i.p.) olarak enjekte edildi (n=5), Grup III: Midazolam grubuna 50 mg kg'1 midazolam i.p. uygulandı (n=7). Kontrol grubunda eritrosit deformabilite indeksi 1,26 ± 0,05, çözelti grubunda 1,26 ± 0,2, midazolam grubunda 1,28 ± 0,09 olarak saptandı. Plazma viskozitesi kontrol grubunda 1,53 ± 0,11, çözelti grubunda 1,78 ± 0,5 ve midazolam grubunda 1,45 ± 0,2 olarak bulundu. Gruplar arasında çalışılan tüm parametrelerde istatistiksel olarak anlamlı bir fark saptanmadı (p>0,05). Özellikle uzun dönem, yoğun bakım hastalarında kullanılan midazolamın bu iki parametre üzerinde olumsuz bir etkisi olmadığını gözlemlemek, midazolamın klinik kullanımda güvenilirliğini arttıracaktır.

Anahtar Kelimeler:

Akyuvar biçim değişimi, Midazolam, Plazma, Kan viskozitesi, Sıçan, Wistar

The effects of midazolam on erythrocyte deformability and plasma viscosity in rats

At the microcirculation level erythrocyte deformability and plasma viscosity are the main determinants of the resistance against blood flow. The effects of the propofol and some volatile agents on the erythrocyte deformability have been studied previously. Whereas the effect of midazolam on erythrocyte deformability and plasma viscosity has not been studied. The objective of our study was to determine the effects of midazolam on erythrocyte deformability and plasma viscosity. Twenty Wistar albino rats were randomly divided into three groups. The first group, was the control group, that did not receive any injection. The second (vehicle group) and third groups (Midazolam group) received intraperitoneal injections at equal volumes of either the solvent of midazolam or 50 mg kg-1 midazolam, respectively. The erythrocyte deformability and plasma viscosity in each of the three groups were similar.the erythrocyte deformability index of the control group was 1,26 ± 0,08 where as it was 1,26 ± 0,2 for the solvent group and 1,28 ± 0,09 for the midazolam group. Plasma viscosity was found 1,53± 0,11 in the control group where it was 1,78 ± 0,5 and 1,45 ± 0,2 for the solvent group and midazolam group respectively. Midazolam did not have any effects on these parameters. So, midazolam can be used safely in patients with problems at microcirculation level. These findings might have important clinical implications such as in ischemic brain and heart diseases, intensive care unit sedation of septic shock cases and hyperviscosity syndromes.

Keywords:

Erythrocyte Deformability, Midazolam, Plasma, Blood Viscosity, Rats, Wistar,

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