Hüseyin ÖZVEREN, Mustafa GÜNEŞ, İrfan Şafak BARLAS

İdiyopatik infertil hastalarda semen ve kan plazmasında malondialdehit, glutatyon peroksidaz, süperoksid dismutaz ve katalaz düzeyleri ile semen parametrelerinin ilişkisi

AMAÇ: Biyolojik sistemlerde oksidatif denge bozulduğu zaman oksidatif stres ortaya çıkmaktadır. Lökospermi ve varikosel varlığında seminal plazmada oksidanların artışı, bu patolojilerin spermatozoalar üzerine olası zararlarını açıklamada kullanılan paramet- relerden biridir. İnfertil olguların yaklaşık %30’unu oluşturan idiyopatik İnfertilite’de ise spermiyogram parametrelerindeki bozulmaları açıklamaya yönelik çalışmalar sürmekte- dir. Bu çalışmada infertil hastalarda kan ve semen plazmasında Süperoksid Dismutaz (SOD), Katalaz (CAT), Glutatyon Peroksidaz (GPx), Malondialdehit (MDA) gibi enzim düzeylerinin sperm sayı, hareket, morfolojisi ile olan ilişkisi ve bu ilişki üzerinden idiyo- patik infertiliteyi açıklayabilecek bir mekanizmanın olabileceğini araştırmayı amaçladık. GEREÇ ve YÖNTEMLER: Semen ve plazma örnekleri kliniğimize infertilite nedeniyle başvuran; varikosel, hormonal, lökospermi, obstruktif patolojilerin varlığı gibi bilinen bir nedene bağlı infertilite nedeni olmayan olgulardan alındı. İnfertilite etyolojisi primer bir nedene bağlanamamış idiyopatik infertil 40 hasta değerlendirilmeye alındı. Kontrol grubu olarak son bir yıl içinde çocuk sahibi olan ve semen parametreleri Dünya Sağlık Örgütü (WHO) tarafından 2010 yılında belirlenen semen analizi kriterlerine göre nor- mal olan 20 fertil kişi çalışmaya alındı. Hasta ve kontrollerde kan serumu ve seminal plazmada SOD, CAT, GPx ve MDA düzeyleri ölçüldü ve sperm parametreleri incelendi. BULGULAR: Hasta ve kontrol gruplarının yaşları karşılaştırıldığında aralarında anlamlı bir farklılık saptanmadı. Buna karşın hastaların olduğu grubun sperm sayı (67,07±68,182 vs 215±118,743), hareket (23,17±20,570 vs 47±10,809) ve morfoloji (50,73±29,274 vs 69±9,119) ölçümleri kontrol grubundan anlamlı şekilde düşük bulundu (p

On the idiopathic infertile male patients, level of malondialdehyde, glutathion peroxidase, superoxide dismutase and catalase at sperm and blood plasma, and correlation between them

OBJECTIVE: In biological systems when the oxidative balance is deteriorated, oxidative stress arises. The increase of oxidants in seminal plasma in the presence of leukocytospermia and varicocele is one of the argumants used to explain the potential losses of these pathologies on spermatozoas. As for the “idiopathic infertility” which forms approximately 30% of the infertility cases; there has been ongoing studies to explain deterioration in spermyogram parameters. In this study, we investigated the relationship of parameters such as superoxide dismutase, catalase, glutathione peroxidase, malatdehidrogenaz on blood and sperm plasma of infertile patients with the sperm parameters as well as the existence of a mechanism that explains this relationship over idiopathic infertility. MATERIAL and METHODS: In this study we indeed 40 patients that matches the criteria of idiopathic infertility were evaluated. Those who had children in the past year and whose semen parameters fit the WHO 2010 criteria were used as a control group of 20 people. Blood and seminal plasma superoxide dismutase, catalase, glutathione peroxidase and malatdehidrogenaz levels were measured in patient controls and sperm parameters were examined. RESULTS: Total sperm number (67.07±68.182 vs 215±118.743), motility (23.17±20.570 vs 47±10.809) and morphology (50.73±29.274 vs 69±9.119) measurements in the patient group was found significantly lower than the control group (p

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