Beden kitle indeksinin, klasik sperm parametreleri ve sperm DNA fragmantasyonu ile ilişkisinin incelenmesi: Kesitsel bir araştırma

AMAÇ: Gelişmiş ülkelerdeki yaşam tarzı değişiklileri sebebiyle artan obez ve aşırı kilolu kişilerin sayısı, dünya çapında bir sağlık problemi hale gelmiştir. Obezite, ek komorbid hastalıklara, hormonal bozulmalara, serbest oksijen radikal artışına sebep olarak, üreme fonksiyonunu olumsuz yönde etkilemektedir. Çalışmamızın amacı, beden kitle indeksinin hem klasik sperm parametreleri hem de fonksiyonel sperm kalitesi üzerindeki etkilerini araştırmaktır. GEREÇ ve YÖNTEMLER: Hastanemizde semen analizi yapılan erkekler, ideal ki- lolu (1. Grup), fazla kilolu (2. Grup) ve obez (3. Grup) olmak üzere üç gruba ayrıl- mış, bireylerde klasik sperm parametreleri ve sperm DNA yapısı incelenmiştir. Her üç grupta da standart semen analizinin yanında, sperm DNA fragmantasyon (SDF) testi için akridin oranj, maturasyon analizi için anilin mavisi (AB), sperm kromatin kondensasyonu için kromomisin A3 (CMA3) boyama yöntemi kullanıldı. BULGULAR: Obezitenin, sperm konsantrasyonunu (1. Grup: 55,0 milyon/ml, 2. Grup: 64,2 milyon/ml, 3. Grup: 47,7 milyon/ml; p>0,1) ve ileri hareketliğini (1. Grup: %41,0, 2. Grup: %42,6, 3. Grup: %35,5; p>0,1) istatistiki olarak et- kilemediği görülürken, normal sperm morfolojisi obez grupta daha düşük bulun- muştur (1. Grup: %4,0, 2. Grup: %4,9, 3. Grup: %3,2; p

Investigation of the association between body mass index, classical sperm parameters and sperm DNA fragmentation: A cross-sectional study

OBJECTIVE: The number of over-weight and obese individuals which increase progressively due to the life style changes in developed countries, became a worldwide problem. Obesity has a negative impact on fertility due to comorbid diseases, hormonal disruptions and over production of free oxygen radicals. Our objective was the investigation of the association between body mass index (BMI), classical sperm parameters and sperm DNA integrity. MATERIAL and METHODS: Males who had a semen analysis in our hospital were classified into three groups: Ideal weight group (I), overweight group (II) and obese group (III). Standard semen analysis was done in all three groups. Acridine orange was used for DNA fragmentation test, aniline blue was used for maturation analysis, and chromomycin A3 (CMA3) staining method was used for sperm chromatin condensation. RESULTS: There was no significant different between three groups in terms of sperm concentration (I: 55.0 million/ml, II: 64.2 million/ml, III: 47.7 million/ml; p>0.1) and progressive motility (I: 41.0%, II: 42.6%, III: 35.4%; p>0.1), while normal sperm morphology rate was lower in obese group than the overweight group (I: 4.0%, II: 4.9%, III: 3.1; p

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