YASEMİN PARLAK, Didem GÖKSOY, Bahattin BOZDAĞ, Ali ÖZDEMİR, Gözde MUTEVELİZADE, KAMURAN AKTAŞ, Gül GÜMÜŞER, Elvan SAYİT, Canan ÖZDEMİR

Effects of Radioiodine (I131) on Mitotic Chromosomes of Root Meristem Cells of Vicia faba L. and Numerical Evaluation

In this study Cytotoxic effects of radioiodine (I131) which is a radionuclide used for the diagnosis and treatment of thyroid cancers and hyperthyroidism on mitotic chromosomes of root tips meristematic cells of fava bean [Vicia faba L. (Fabaceae)] were investigated. Seeds treated with radioiodine (I131) for 24, 48, 96 hours and 16 days were germinated. The root tips were taken and prepared for microscopic studies. Radioiodine (I131) caused chromosomal abnormalities as chromosome adherence, chromosome breaks, ring chromosome, chromatin bridge in V. faba L. root tip cells. The most common type of observed abnormalities was fish bone chromosome adherence, ring and C chromosome and chromosome dispersion. As a result of this study, it was determined that radioiodine (I131) had a mitodepressive effect on mitosis and harmful effects on V. faba L. root tip cells. The data obtained were evaluated numerically and statistical analysis was performed.

Radyoiodinin (I131) Vicia faba L.'nın Kök Meristem Hücrelerinin Mitotik Kromozomları Üzerindeki Etkileri ve Sayısal Olarak Değerlendirilmesi

Bu çalışmada tiroid kanserlerinin ve hipertiroidizmin tanı ve tedavisinde kullanılan bir radyonüklid olan radyoiodinin (I131), baklanın [Vicia faba L. (Fabaceae)] kök uçlarındaki meristematik hücrelerinin mitotik kromozomları üzerindeki sitotoksik etkileri incelendi. Tohumlar radyoiodin ile 24, 48, 96 saat ve 16 gün sürelerle muamele edildi ve çimlendirildi. Çimlenen kök uçları alındı ve mikroskobik çalışmalar için hazırlandı. Radyoiodine (I131) V. faba L. kök ucu hücrelerinde kromozom yapışması, kromozom kopması, halka kromozom yapışması, köprü kromozom gibi kromozom anormalliklerine neden oldu. En çok görülen anormallikler balık kılçığı kromozom yapışması, halka ve C kromozom, kromozom dağılmasıdır. Bu çalışmanın sonucu olarak, radyoiyodinin mitoz üzerinde mitodepresif bir etkisi olduğu ve V. faba L. kök ucu hücreleri üzerinde zararlı etkileri olduğu tespit edildi. Elde edilen veriler sayısal olarak değerlendirilip istatistiksel analizleri yapıldı.

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