The Variability of the Predominant Culturable Plant GrowthPromoting Rhizobacterial Diversity in the Acidic Tea Rhizosphere Soils in the Eastern Black Sea Region

The purpose of this study was to investigate the diversity of cultivable nitrogen fixing, phosphate solubilising and total bacteria originated from 580 rhizospheric acidic soils samples of tea plants grown at 62 locations. Based on FAME profiles of over 1428 rhizoplane bacteria, 63 bacterial genera were identified with a similarity index > 0.3, but 56.4% of the identified isolates belonged to six genera: Bacillus (37.02%), Pseudomonas (12.67%), Stenotrophomonas (5.71%), Paenibacillus (6.58%), Arthrobacter (4.35%) and Brevibacillus (3.98%). Most of the total, N2-fixing and P-solubilizing bacteria isolated were Gram positive (59.9, 58.8 and 56.3%) and Gram negative constituted only 40.1, 41.2 and 43.7%. Among different groups, Firmicutes, Gammaproteobacteria and Actinobacteria comprised the largest groups contributing to about 50.3 and 46.6%, 30.8 and 32.5%, and 8.3 and 9.6% of the total N2-fixing and P-solubilizing isolates, respectively. B. cereus, P. fluorescens, B. megaterium, S. maltophilia, P. putida, B. licheniformis, B. pumilus, B. subtilis and P. polymyxa were the most frequent N2-fixing and P-solubilizing species in the acidic tea rhizosohere soils. In these studies were evaluated to represent the dominant culturable diversity of diazotrophs and phosphobacteria, and thus potentially beneficial to the growth and survival of tea plants in that specific acidic ecosystem of eastern Black Sea region.

Kaynakça

Asghar, H.N., Zahir, Z.A., Arshad, M. and Khaliq, A. 2002. Relationship between in vitro production of auxins by rhizobacteria and their growth-promoting activities in Brassica juncea L. Biology and Fertility of Soils 35:231- 237.

Beneduzi, A., Peres, D., da Costa, P.B., Bodanese Zanettini, M.H. and Passaglia, L.M.P., 2008. Genetic and phenotypic diversity of plant-growth-promoting bacilli isolated from wheat fields in southern Brazil. Research in Microbiology 159: 244-250.

Borsodi, A.K., Makk, J., Rusznyák, A., Vajna, B., Taba, G., and Márialigeti, K., 2007. Phenotypic characterization and molecular taxonomic studies on Bacillus and related isolates from Phragmites australis periphyton. Aquatic Botany 86: 243-252.

Çakmakçi, R., Dönmez, F., Aydin, A., and Sahin, F., 2006. Growth promotion of plants by plant growth-promoting rhizobacteria under greenhouse and two different field soil conditions. Soil Biology & Biochemistry 38: 1482- 1487.

Çakmakçı, R., Erat, M., Erdoğan, Ü., and Dönmez, F., 2007. The influence of plant growth-promoting rhizobacteria on growth and enzyme activities in wheat and spinach plants. Journal of Plant Nutrition and Soil Science 170: 288-295.

Çakmakçı, R., Dönmez, M.F., Ertürk,Y., Erat, M., Haznedar, A., and Sekban, R., 2010. Diversity and metabolic potential of culturable bacteria from the rhizosphere of Turkish tea grown in acidic soils. Plant and Soil 332: 299-318.

Chen, Y.P., Rekha, P.D., Arun, A.B., Shen, F.T., Lai, W.-A., and Young, C.C., 2006. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Applied Soil Ecology 34: 33-41.

Costa, R., Götz, M., Mrotzek, N., Lottmann, J., Berg, G., and Smalla, K., 2006. Effects of site and plant species on rhizosphere community structure as revealed by molecular analysis of microbial guilds. FEMS Microbiology Ecology 56: 236–249.

Döbereiner, J., 1989. Isolation and identification of root associated diazotrophs, in: F.A. Skinner (Ed.), Nitrogen Fixation with Non-Legumes, Kluwer Academic Publishers, Dordrecht, Boston, London, pp. 103-108.

Donate-Correa, J., León-Barrios, M., and Pérez-Galdona, R., 2004. Screening for plant growth-promoting rhizobacteria in Chamaecytisus proliferus (tagasaste), a forage tree-shrub legume endemic to the Canary Islands. Plant and Soil 266: 261-272

Fierer, N., Jackson, R.B., 2006. The diversity and biogeography of soil bacterial communities. Proceedings of the National Academy of Sciences 103: 626–631.

Hariprasad, P., and Niranjana, S.R., 2009. Isolation and characterization of phosphate solubilizing rhizobacteria to improve plant health of tomato. Plant and Soil 316: 13-24

Karagöz, K., Ateş, F., Karagöz, H., Kotan, R., and Çakmakçı, R., 2012. Characterization of plant growth-promoting traits of bacteria isolated from the rhizosphere of grapevine grown in alkaline and acidic soils. European Journal of Soil Biology 50:144-150.

Oka, N., Hartel, P.G., Finlay-Moore, O., Gagliardi, J., Zuberer, D.A., Fuhrmann, J.J., Angle, J.S., and Skipper, H.D., 2000. Misidentification of soil bacteria by fatty acid methyl ester (FAME) and BIOLOG analyses. Biology and Fertility of Soils 32: 256–258.

Pikovskaya RE (1948) Mobilization of phosphates in soil in connection with vital activities of some microbial species. Microbiologia 17:362-370.

Piromyou, P., Buranabanyat, B., Tantasawat, P., Tittabutr, P., Boonkerd, N., and Teaumroong, N., 2011. Effect of plant growth promoting rhizobacteria (PGPR) inoculation on microbial community structure in rhizosphere of forage corn cultivated in Thailand. European Journal of Soil Biology 47: 44-54.

Poonguzhali, S., Madhaiyan, M., and Sa T., 2006. Cultivationdependent characterization of rhizobacterial communities from field grown Chinese cabbage Brassica campestris ssp pekinensis and screening of traits for potential plant growth promotion. Plant and Soil 286:167-180.

Rau, N., Mishra, V., Sharma, M., Das, M.K., Ahaluwalia, K., and Sharma, R.S., 2009. Evaluation of functional diversity in rhizobacterial taxa of a wild grass (Saccharum ravennae) colonizing abandoned fly ash dumps in Delhi urban ecosystem. Soil Biology & Biochemistry 41: 813- 821.

Şahin, F., Çakmakçi, R., Kantar, F., 2004. Sugar beet and barley yields in relation to inoculation with N2-fixing and phosphate solubilizing bacteria. Plant and Soil 265:123- 129.

Varmazyari, A., and Çakmakçı, R., 2018. Diversity and metabolic potential of the dominant culturable N2- fixing and P-solubilising bacteria from tea (Camellia sinensis L.) rizosphere. Frontiers in Environmental Microbiology 4(2): 45-54.

Xie, G.H., Cai, M.Y., Tao, G.C., and Steinberger, Y., 2003. Cultivable heterotrophic N2-fixing bacterial diversity in rice fields in the Yangtze River Plain, Biology and Fertility of Soils 37:29-38.

Xue, D., Yao, H.Y., Ge, D.Y., Huang, C.Y., 2008. Soil microbial community structure in diverse land use systems: A comparative study using Biolog, DGGE, and PLFA analyses. Pedosphere 18: 653-663.

Xue, D., Yao, H., and Huang, C. Y., 2006. Microbial biomass, N mineralization and nitrification, enzyme activities, and microbial community diversity in tea orchard soils. Plant and Soil 288:319-331.

Kaynak Göster

301 193

Arşiv
Sayıdaki Diğer Makaleler

The Determination of Microbiological Properties of Rainbow Trout (Oncorhynchus mykiss) Applied with Black Cumin Oil in Different Concentrations

GÖKHAN ARSLAN

The Effects of Different Intensity of Thinning on the Development in Scots Pine (Pinus sylvestris L.) Stands in Kazakh Uplands

Andrei V. EBEL, Yekaterina I. EBEL, Sergei V. ZALESOV, Sezgin AYAN

A New Financing Model for Carbon Emission Reduction Projects: The Use of Carbon Emission Reduction Purchase Agreements (ERPA) in the Private Pension System

GÖKHAN ŞEN, MEHMET YUNUS ÇELİK, TOLGA ULUSOY

Effect of Spirulina platensis (Gomont) Geitler Extract on Seed Germination of Wheat and Barley

FÜSUN AKGÜL

Determination of Amino Acids Composition in Different Tissues of Whiting, Merlangus merlangus euxinus (Nordmann, 1840) from the Black Sea, Turkey

ÖZLEM BİLGİN, Uğur ÇARLI, Selahattin ERDOĞAN, Murat Emrah MAVİS, Gökçe GOKSU GURSU, Muhittin YILMAZ

Maximum Length Record of Common Two-banded Seabream (Diplodus vulgaris Geoffroy Saint-Hilaire, 1817) for Aegean Sea with Turkish Waters

ÖZGÜR CENGİZ, ŞÜKRÜ ŞENOL PARUĞ, BAYRAM KIZILKAYA

Effects of Lovastatin Suplemented Diet on Laying Performance, Egg Quality, Yolk Lipid Profile and Some Serum Parameters in Laying Hens

Özlem EKİNCİ, ADEM KAYA, HATİCE KAYA, NURİNİSA ESENBUĞA, MUHLİS MACİT

Association between the Crab, Nepinnotheres pinnotheres (Linnaeus, 1758), and the Endangered Species Fan Mussel, Pinna nobilis (Linnaeus, 1758), from the Aegean Sea, Turkey

SEFA ACARLI, PERVİN VURAL, Ahmet ÖKTENER

Effect of Swirl Plates on Volumetric Discharge Rate and Spray Characteristics of Hollow Cone Nozzles

BAHADIR SAYINCI, Ruçhan ÇÖMLEK, BÜNYAMİN DEMİR, Mustafa ÇOMAKLI

Effect of Garlic (Allium sativum L.) on the Microbiological, Chemical and Sensorial Quality of Smoked Atlantic Mackerel (Scomber scombrus Linnaeus, 1758) Stored in Vacuumed Packets at Refrigerator Temperature (+4ºC)

HÜNKAR AVNİ DUYAR, Zafer Hakan KALAYCI, SABRİ BİLGİN