Moleküler markörlerin prunus türlerinde kullanımı

DNA teknolojilerinin geliştirilmesi bitki ıslahı, genetiği ve evrimi konularında çalışan araştırıcılara büyük avantajlar sağlamıştır. Analizler, izoenzim, RFLP, RAPD ve/veya mikrosatellit (SSR) markörlerini kullanarak fenotipten genotipe doğru kaymıştır. Bu yazıda moleküler markörlerin Prunus türlerinde kullanımınajdeğinilmiştir. Araştırmalar; (1) markörler yardımı ile seleksiyon (2) genom haritalaması (3) karşılaştırmalı haritalama (4) gen kaynaklarının karakterizasyonu (5) filogenetik analizler ve kültür çeşitlerinin tanımlanması konularından verilmiştir.

Anahtar Kelimeler:

Prunus, gen haritalaması, moleküler biyoloji, bitki ıslahı, filogenetik, germplazma, DNA

Utilization of molecular markers in Prunus species

The development of DNA technologies has provided great advantage to researchers working on plant breeding, genetics and evaluation. Analyses shifted from phenotype to genotype, using isoenzymes, RFLPs, RAPDs and/or microsatellites (syn. simple sequence repeats, SSRs) as markers. The objective of this review is to outline some important usage of molecular markers in Prunus. Examples include following topics (1) marker assisted selection (2) genom mapping (3) comparative mapping (4) characterization of germplasm (5) phylogenetic analysis and cultivar identification.

Keywords:

Prunus, gene mapping, molecular biology, plant breeding, phylogenetics, germplasm, DNA,

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