Melissa officinalis L. Uçucu Yağ Kompozisyonunun ve Amaranthus retroflexus L. Tohumlarının Çimlenmeleri Uzerine Etkilerinin Belirlenmesi

Son yıllarda doğal bitki bileşenlerinin birçok alanda kullanıldığı bilinmektedir. Kolay bir şekilde metabolize edilmeleri birçok alanda sentetik kimyasallar yerine kullanımlarının önünü açmıştır. Bu çalışmada; birçok alanda kullanılan, sekonder metabolitlere sahip Lamiaceae bitki familyasına ait olan Melissa ajficinalis türünden uçucu yağlar elde edilmiştir. Elde edilen uçucu yağların içerik analizleri GC--MS (Gas chromatography--mass spectrometry) yöntemiyle belirlenmiştir. Uçucu yağlar daha sonra farklı dozlarda Amaranthus retroflexus bitkisinin çimlenen tohumlarına uygulanmış ve tohumlar üzerine muhtemel genotoksik ve fizyolojik etkileri belirlenmiştir. Elde edilen verilere göre türün majör bileşen olarak %11.26 oranında E--Citral, %9.74 (--)-- Caryophyllene Oxide, %9.07 Z--Citral ve %3.64 oramnda Prohydrojasmon--2 içerdiği belirlenmiştir. 0.2 ve 0.4 ul/mL dozları dışında, uçucu yağların artan doza bağlı olarak çimlenen Amaranthus retraflexus tohumlarının kök uzunluklarını ve genomik stabilitelerini düşürdüğü gözlenmiştir.

Determination of the Chemical Composition of the Essential Oil of Melissa officinalis and Their Effects on Germination of Amaranthus retroflexus

It is known that the natural occurred plant compounds are used in many fields in recent years. Because of their easily metabolization, the use of these chemicals paved the way for their use instead of synthetic chemicals in many areas. In the current study, essential oils of Melissa officinalis species of Lamiaceae family were obtained. The obtained essential oil content was analysis by GC-MS (gas chromatography-mass spectrometry) method. The oils applied to the seedlings of Amaranthus retroflexus plants in different doses. Then the genotoxic potential and physiological effects of oils have been identified. According to the data obtained from GS-MS, E-Citral (11.26%), (-) - caryophyllene oxide (9.74%), Z-Citral (9.07%) and Prohydrojasmon-2 (3.64%) was determined as major components. It is also determined that, the essential oils have reduced both root length and genomic template stability of the Amaranthus retroflexus seedlings except 0.2 ve 0.4 µl/mL doses.

Kaynakça

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