Fatma Gül GÖZE ÖZDEMİR, Tuğçe ÖZEK, Jean Claude NDAYIRAGIJE, Hacer ÇEVİK, İsmail KARACA

Domateste Kök Ur Nematodu Mücadelesinde Kitosanın Toprak ve Yaprak Uygulamaları

Bu çalışmada kontrollü koşullarda (24 ± 1°C, %60 ± 5 nem) domates bitkisinde kitosanın toprak, yaprak ve eş zamanlı toprak+yaprak uygulamalarının kök ur nematodu Meloidogyne incognita’nın gal ve yumurta paketi üzerindeki etkisi araştırılmıştır. Pozitif kontrol olarak Fluopyram (0.16 ml/L), negatif kontrol olarak sadece nematod uygulanan bitkiler kullanılmıştır. Çalışma her bir uygulama için tesadüf parselleri deneme deseninde 5 tekerrürlü olarak kurulmuştur. Çalışmada kitosanın %1 oranında seyreltilmiş sıvı süspansiyonu kullanılmıştır. Toprak uygulamasında her saksıya 5 ml mezür ile uygulanırken, yaprak uygulamasında ise hazırlanan %1’lik sıvı süspansiyonu portatif el püskürtücü kullanılarak çözelti bitene kadar uygulanmıştır. Çalışma nematod inokulasyonundan 60 gün sonra 1-9 gal ve yumurta paketi indeksine göre değerlendirilmiştir. En düşük gal (2.8) ve yumurta paketi (2.4) indeksi eş zamanlı toprak+yaprak uygulamasında tespit edilmiş ve pozitif kontrol nematisitle aynı istatistiki grupta yer almıştır. Kitosanın toprak ve yaprak uygulamasının beraber yapılması durumunda nematisidal etkinin arttığı belirlenmiştir. Bu sonuçlar kitosan uygulamalarının kök ur nematodu kontrolünde başarılı olduğunu desteklemektedir.

Anahtar Kelimeler:

Kitosan, kök ur nematodu, biyolojik kontrol, uyarılmış dayanıklılık

Soil and Foliar Applications of Chitosan in the Control of Root-Knot Nematode in Tomato

In this research, the effect of soil, foliar and simultaneous soil+foliar applications of chitosan on gall and egg mass of root-knot nematode Meloidogyne incognita was investigated in tomato plants under controlled conditions (24±1°C, 60±5% RH). Fluopyram (0.16 ml/L) was used as positive control while only nematode-treated plants were considered as negative control. The study was set up in a randomized plot design with 5 replications for each application. The liquid suspension of chitosan diluted at 1% was used. In soil application, 5 ml was applied to each pot by using a graduated cylinder while in foliar application, the liquid suspension at 1% was applied using a portable hand sprayer until the solution was finished. The study was evaluated according to the 1-9 gall and egg mass index 60 days after inoculation of nematodes. The lowest gall (2.8) and egg mass (2.4) indexes were detected in the simultaneous soil+foliar application and took place in the same statistical group with the positive control nematicide. It was determined that the nematicidal effect of chitosan has increased in the case of combined soil and foliar applications. These results support the fact that chitosan applications are successful in the control of root-knot nematode.

Keywords:

Chitosan, root-knot nematode, biological control, induced resistance,

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