Tek doz aşı uygulaması yapılmış hindilerde newcastle hastalığı virusu antikorların hemaglutinasyon inhibisyon (HI) testi ile saptanması
Bu çalışmada, içme suyu yoluyla 30. günde bir kez canlı La Sota aşısı uygulanmış et tipi hindi üretimi yapılan bir işletmede, dişi ve erkek hayvanların kesim dönemindeki Newcastle hastalığı virüsü antikorlarının hemaglutinasyon inhibisyon (HI) testi ile belirlenmesi amaçlandı. HI testi U tabanlı mikropleytlerde, reagentlar 50 $mu$ l, antijen ise 8 hemaglutinasyon ünitesinde (S HAÜ) kullanılarak yapıldı. Çalışmada biri dişi, ikisi de erkek hindi kümeslerinden olmak üzere toplam 960 serum örneği, dişilerden 105., erkeklerden ise 120. günde alınarak test edildi. Birinci kümesten (erkek) 271, 2. kümesten (dişi) 320, 3. kümesten de (erkek) 369 serum örneği incelendi. HI testi ile 1. kümesten 140 (% 51.6), 2. kümesten 304 (% 94.8), 3. kümesten ise 189 (% 51.2) hayvan pozitif olarak değerlendirildi. Sürü ortalama antikor titreleri 1. kümeste 6.3, 2. kümeste 9.7, 3. kümeste 5.9 olarak belirlendi. HI testinde 7 ($log_2$) değerinin altında ve üstünde antikor titresine sahip olan hayvanlar arasındaki fark, 1. ile 3. kümes arasında önemli bulunmazken (P>0.05), 2. kümes ile 1. kümes (P
Anahtar Kelimeler:
hindi, aşı(hayvan), dozaj etkileri, yalancı tavuk vebası, hemaglütinasyon inhibisyon testi, antikor
Detection of antibodies againts newcastle disease virus vaccinated with a single dose in Turkeys by hemagglutination inhibition (HI) test
The aim of this study was to determine serum antibody levels against Newcastle disease virus vaccinated at 30 day of age by drinking water with a single dose of chicken live La Sola strain Newcastle disease vaccine in slaughter time in male and female meat turkey flocks by hemagglutination, inhibition (HI) test. HI test were performed on U shaped microtiter plates using a total volume of 50 /M. test reagent and 8 heniagglutination units (HAV) of antigen. For this purpose a total of 960 blood sera were collected from three field meat turkey flocks. Sera samples were obtained from one female flock slaughtered in day 105 and two male flocks slaughtered in day 120. 271 samples from the 1st flock (male), 320 from the 2nd flock (female) and 396 from the 3th flock (male) were taken for the test. According to this evaluation 140 (51.6 %), 304 (94.8 %) and 189 (51.2 %) sera found to be positive and the HI antibody liters of flocks were 6.3, 9.7 and 5.9 by HI test; 1st, 2nd and 3th flock, respectively. The chi-square test was used for the statistical analyses of HI test results. When the results of the tested sera were compared the difference on the base of 7 ($log_2$) HI liter, between 1st and 3th of flocks were found to be statistically insignificant (P>0.05), whereas highly significant differences (P<0.001) were found between 2nd flock and both 1st and 3th flock. This serologic.al investigation showed that antibody liter of female meat turkey flock were over 7 (Iog2) HI tiler, whereas male meat turkey flocks antibody liters were below this liter. The results of the present study indicate that only once vaccinated male turkeys were susceptible to Newcastle disease at 13 weeks after vaccination. Therefore, alterations in the ongoing vaccine strategies are to be made. In conclusion, the meat turkeys produced more than 120 days in the epidemic area of Newcastle disease may require the boosting vaccination for the adequate protection from the infection.
Keywords:
turkeys, vaccines, dosage effects, Newcastle disease, haemagglutination inhibition test, antibodies,
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- ISSN: 1017-8422
- Yayın Aralığı: 3
- Başlangıç: 1990
- Yayıncı: Yüzüncü Yıl Üniv. Veteriner Fak.