Yabani Aspir'in (Carthamus persicus Wild) in vitro Çoğaltımında Farklı BAP-NAA Kombinasyonlarının Etkisi

Aspir, linoleik asit ve oleik asitce zengin; yenilebilir doymamış yağ asitlerinin elde edilmesinde kullanılması nedeniyle önemli bir endüstriyel bitkidir. Bu çalışmada aspirin in vitro’ da çoğaltımı amacıyla sürgün ucu eksplantları 0.25- 0.50- 1- 2 mg/L BAP ve 0.25- 0.50-1 mg/L NAA’ nın farklı kombinasyonlarını içeren, %3 sakkaroz ilave edilmiş ve % 0.8 agar ile katılaştırılmış MS ortamında kültüre alınmıştır. Araştırma sonuçları kallus oluşumu, rejenere sürgün yüzdesi, eksplant başına sürgün sayısı, sürgün uzunluğu, eksplant başına kök sayısı ve kök uzunluğu için BAP-NAA arasındaki kombinasyoların etkili olduğunu göstermiştir. Maksimum sürgün rejenerasyonu 0.50 mg/L BAP + 0.25 mg/L NAA içeren MS besin ortamında, maksimum kallus oluşumu ise 1 mg/L BAP + 1 mg/L NAA ve 2 mg/L BAP + 1mg/L NAA içeren MS besin ortamında kaydedilmiştir. Rejenere sürgünler 1 mg/L IBA içeren MS besin ortamında köklendirilmiştir.

Anahtar Kelimeler:

BAP, NAA, Aspir, Mikroüretim

Effect of Different Combinations of BAP and NAA in vitro Propagation of Wild Safflower (Carthamus persicus Wild)

Safflower (Carthamus persicus Wild), is rich in terms of linoleic acid and oleic acid and is used to obtain low saturated fatty acid. In order to propagate safflower in vitro, shoot tip explants were cultured in MS medium containing different combinations of BAP and NAA supplemented with 3% sucrose and solidified with 0.8% agar. The results showed a significant interaction between BAP and NAA combinations for callus formation, shoot and root regeneration, number of shoots and roots per explant, shoot and root length. Maximum shoot regeneration was noted in MS medium containing 0.50 mg/L BAP + 0.25 mg/L NAA. Maximum callus formation was determined in MS medium supplemented with 1 mg/L BAP + 1 mg/L NAA and 2 mg/L BAP + 1mg/L NAA. The regenerated shoots were rooted on MS medium containing 1 mg/L IBA.

Keywords:

BAP, NAA, Safflower, Micropropagation,

PDF

___

Başalma D, Uranbey S, Mirici S, Kolsarici O (2007). TDZ x IBA induced shoot regeneration from cotyledonary leaves and in vitro multiplication in safflower (Carthamus tinctorius L.). African Journal of Biotechnology, 7: 960-966.
FAO (2013). http://faostat.fao.org.
Kadıoğlu A (2004). Bitki Fizyolojisi. Eser ofset matbacılık, 258-317, Trabzon.
Konar V, Aşkın Y, Türkoğlu İ (2010). Yabani Aspir (Carthamus persicus Wild) Bitkisinin Yağ Asidi Bileşiminin İncelenmesi. Fırat Üniv. Fen Bilimleri Dergisi, 22: 29-36.
Lincoln T, Eduardo Z (2008). Bitki fizyolojisi 3. Baskıdan tercüme. Palme yayıncılık, 493-515, Ankara. Mariashibu TS, Anbazhagan VR, Jiang SY, Ganapathi A, Ramachandan S (2013). In vitro regeneration and genetic transformation of soybean, edited by James E. Board, published: January 2.
Mensink RP, Temme EHM, Hornstra G (1994). Dietary saturated and trans fatty acids and lipoprotein metabolism. Annals of Medicine, 26: 461-464.
Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
Nikam TD, Shitole MG (1998). In vitro culture of safflower L. cv. Bhima: Initiation, growth optimization and organogenesis. Plant Cell Tissue and Organ Culture, 55: 5-22.
Okamoto K, Machida M, Yamaquchi A, Arimoto-Kabayashi S (2005). Evolation of photo-mutagenicity and photo-cytotoxicity of food coloring agent. Mutagenesis, 20: 229-233.
Özdemir FA, Aymelek F, Karataş F (2011). Aspir (Carthamus persicus Wild) bitkisinde redükte, okside glutatyon ile A, C, E vitamini ve β-karoten miktarlarının araştırılması. Fırat Üniv. Fen Bilimleri Dergisi, 23: 71-76.
Özgen M, Özcan S, Sevimay CS, Sancak C, Yıldız M (1998). High frequency adventitious shoot regeneration in sainfoin. Plant Cell Tissue and Organ Culture, 42: 205-208.
Radhika K., Sujatha M, Nageshwar RT (2006). Thidiazuron stimulates adventitious shoot regeneration in different safflower explants. Biologia Plantarum, 50: 174-179.
Sales RL (2005). The effects of peanut safflower and olive oil on body composition, energy metabolism, lipid profile and food intake of eutrophic, normolipidemic subjects. Revista Nutrican, 18: 4995
Sujatha M, Dinesh KV (2007). In vitro bud regeneration of Carthamus tinctorius and wild Carthamus species from leaf explants and axillary buds. Biologia Plantarum, 51: 782-786.
Talat K, Anwar SY (2010). High frequency somatic embryogenesis and plantlet regeneration via somatic embryos in safflower (Carthamus tinctorius L.). Biosciences Biotechnology Research Asia, 7: 239-2
Vogel G, Browse J (1996). Cholinephosphottansferase and diacylglycerolacyltransferase (substrate specifities at a key branch point in seed lipid metabolism). Plant Physiology, 110: 923-931.
Walia N, Kaur A, Babbar SB (2007). Proliferation and differentiation from endosperms of Carthamus tinctorius. Biologia Plantarum, 51: 749-753.