Sadegh TAGHIPOUR, Amir RAHIMI, Mohammad ZARTOSHTI, Yusuf ARSLAN

Mikro Besin Maddelerinin Humik Asit Koşulları Altındaki Kekik (Thymus vulgaris L.)’in Antioksidan Özelliklerine Etkisi

Bu çalışmanın amacı, mikro besin maddelerinin (Fe, Zn, B ve Mn) humik asit altındaki kekiğin antioksidan aktivitesi üzerine olan özel etkilerini araştırmaktır. Araştırma, 2015-2016 yılları arasında İran-Batı Azerbaycan, Urumiye Üniversitesi Ziraat Fakültesi Tarla Bitkileri Bölümü Deney Alanları ve Laboratuvarında gerçekleştirildi. Deneme tesadüfi blok deseninde ve üç tekerrürlü olarak yürütülmüştür. Araştırmada, her parsele her sulamada 3 gr Fe/L, Mn 3 gr Mn/L, 4 gr B/L, ve 2 gr Zn /L ve hümik asit (hektar başına 300 kg) olarak uygulanmıştır. Besin elementleri uygulamaları 3 defa yapılmıştır. Kekik yapraklarında toplam fenolik içeriği, toplam flavonoid içeriği, DPPH radikal giderimi aktivitesi, nitrik oksit radikal giderim aktivitesi ve Zincir kırma aktivitesi gibi bazı antioksidan özellikler belirlenmiştir. İlk hasatın sonuçları, B ile ilgili en yüksek flavonoid (0.84 mg quercetin / g kuru ağırlık) ve nitrik oksit radikal giderme aktivitesinin (% 58.28) en yüksek olduğunu ortaya koymuştur. Mn, Kontrol ve Zn ile ilgili sırasıyla en yüksek toplam fenol içeriği (31.92 mg Gallik asit / g kuru ağırlık), DPPH radikal süpürme aktivitesi (% 92.5) ve zincir kırma aktivitesi (74.25-Abs-3 / dak / mg ekstrakt) belirlenmiştir. İkinci hasatın sonuçları, B + Humik asit ile ilgili en yüksek toplam fenol içeriğinin (40.05 mg Gallik asit / g kuru ağırlık), flavonoidin (0.65 mg kerceretin / g kuru ağırlık) ve DPPH radikal temizleme etkinliğinin (% 87.36) en yüksek olduğunu ve kontrolle ilgili en yüksek zincir kırma aktivitesi (37.43-Abs-3 / dak / mg ekstrakt) olduğunu göstermiştir. Sonuç olarak, B, bitkinin antioksidan aktivitesini artırmak için diğer uygulamalara kıyasla olumlu etkiye sahip olmuştur.

Anahtar Kelimeler:

Antioksidan aktivite, Yaprak Uygulaması, Humik asit, Mikro besin maddeleri, Kekik

The Effect of Micronutrients on Antioxidant Properties of Thyme (Thymus vulgaris L.) under Humic Acid Using Condition

The aim of the study was to investigate the special effects of micronutrients (Fe, Zn, B, and Mn) under humic acid using condition on Thyme antioxidant activity. The trial conducted at the Experimental Fields and laboratory of the Agronomy Department, Faculty of Agriculture, Urmia University, West Azerbaijan, Iran, during 2015-2016. Experiment was set in randomized complete block design and in three replications. In the trial the treatments were included 3 g Fe L-1, 3 g Mn L-1, 4 g B L-1 and 2 g Zn L-1 in each time and humic acid (300 kg per hectare) were given to all plots. Spraying of the elements was done at three times: Some antioxidant properties of thyme leaves such as total phenolic content, total flavonoid content, DPPH radical scavenging activity, nitric oxide radical scavenging activity and Chain-breaking activity were determined. The results of first harvest showed that the highest flavonoid (0.84 mg quercetin/ g dry weight) and nitric oxide radical scavenging activity (58.28 %) related to B. The highest total phenol content (31.92 mg Gallic acid/g dry weight), DPPH radical scavenging activity (92.5%) and chain-breaking activity (74.25-Abs-3 /min/mg extract) related to Mn, Control and Zn, respectively. The results of second harvest showed that the highest total phenol content (40.05 mg Gallic acid/g dry weight), flavonoid (0.65 mg quercetin/ g dry weight) and DPPH radical scavenging activity (87.36%) related to B + Humic acid, and the highest chain-breaking activity (37.43-Abs-3 /min/mg extract) related to control. In conclusion B has positive effect on increasing the activity of antioxidant of the plant than other treatments.

Keywords:

Antioxidant Activity, Foliar Application, Micronutrients, Humic acid, Thyme,

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