Fahimeh MIRZAL, Ebrahim Dorani ULIAIE, Ali Bandeh HAGH

Araştırma Makalesi/Research Article (Original Paper) Stimulation Effect of AgNO3 and CoCl2 as Ethylene Inhibitors on in- Vitro Organogenesis of Sunflower (Helianthus annuus L.)

Özet: Ayçiçeğinde (Helianthus annuus L.) etkili ve güvenilir bir organogenez protokoluna ulaşmak için, etilen inhibitörleri olarak AgNO3 ve CoCl2 nin etkisi araştırılmıştır. CMS19 ve Progress çeşitlerinden elde edilen kotiledon eksplantları 2.0 mg/L BAP ve 1.0 mg/L IAA içeren MS ortamına farklı konsantrasyonlarda AgNO 3 (0, 1.5 ve 3 mg/L) ve CoCl2(3 ve 6 mg/L) ilave edilerek kültüre alınmıştır. Yapılan istatistik analizine göre; eksplant başına sürgün sayısı ve sürgün rejenerasyonunu içeren organogenez parametrelerinde etilen inhibitörlerinin konsantrasyonu ve çeşitler arasında istatistiksel olarak önemli farklılıklar bulunmuştur. Kültür ortamına etilen inhibitörlerinin eklenmesi, eksplant başına sürgün sayısını ve rejenerasyon frekansını artırmıştır. Sürgün rejenerasyonu CoCl2 konsantrasyonun artışıyla artmış, ancak AgNO3 konsantrasyonunun artışına bağlı olarak azalmıştır. Düşük konsantrasyonda (1.5 mg/L) AgNO 3 uygulaması ayçiçeğinde (Helianthus annuus) organogenez artırmıştır. En yüksek sürgün rejenerasyonu ortama 6 mg/l CoCl2 (R/E=% 63 ve S/RE= 3) ve 1.5 mg/L AgNO3 (R/E=% 73 ve S/RE= 2) eklendiğinde elde edilmiştir. Bu araştırmada etilen inhibitörlerinin, özellikle CoCl2nın ayçiçeğinde bitki transformasyon protokolu ve in vitro sürgün rejenerasyon etkinliğinin iyileştirilmesinde kullanılabileceği önerilmiştir.

Ayçiçeği (Helianthus annuus L.) nin in vitro Organogenezi Üzerine Etilen İnhibitörleri Olarak AgNO3 ve CoCl2 nin Uyarıcı Etkisi

Abstract :To achieve an effective and reliable shoot organogenesis protocol in Helianthus annuus L., effect of ethylene inhibitors including AgNO3 and CoCl2 were investigated. Cotyledonary explants of two -1cultivars including CMS19 and Progress were cultured in MS medium with 2.0 mg L BAP and 1.0 mg L -1 IAA supplemented with different concentrations of AgNO3 (0, 1.5 and 3 mg L-1) and CoCl2 (3 and 6 mg L-1). Statistical analysis of data showed a significant difference among cultivars and ethylene inhibitors concentrations for organogenesis parameters including shoot regeneration and number of shoots per explant. Addition of ethylene inhibitors to culture medium improved regeneration frequency and number of shoots per explant. Shoot generation was improved with increasing concentrations of CoCl 2 but in the case of AgNO3, increasing the concentration gave adverse results. Treatment of explants with AgNO3 in low concentration (1.5 mg L-1) improved organogenesis in Helianthus annuus. The highest -1shoot regeneration was obtained in media supplemented with 6 mg L CoCl2 (R/E= 63% and S/RE= 3) and 1.5 mg L-1 AgNO3 (R/E= 73% and S/RE= 2).This study suggests that ethylene inhibitors, particularly CoCl2, could be used to improve the efficiency of in vitro shoot regeneration and plant transformation protocols of sunflower.

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