Sığırlarda Enfeksiyöz Solunum Sistemi Hastalıkları Kompleksinde (BRDC) Klinik, Hematoloji, Biyokimya, Oksidatif Stres, Akut Faz Proteinler Üzerinde Araştırmalar

Bu çalışmada, sığırlarda solunum sistemi kompleksi (BRDC) hastalığında klinik, tedavi, akut faz proteinler, oksidant ve anti oksidant durumu, serum biyokimyası ve hematolojik parametrelerdeki değişmeler araştırıldı. Çalışmada toplam 30 sığır kullanıldı. Çalışma kapsamına alınan hasta hayvanlar iki gruba ayrıldı. I. Gruptaki hayvanlara (n=10), tulatromycin +Flunixin meglumin + Vitamin E ve selenyum kombinasyonu uygulandı. II. gruptaki hayvanlara (n=10) tulatromycin+ flunixin meglumin uygulandı. Kontrol grubundan (n=10) tek sefer, hasta gruplarda tedavi öncesi (0. gün) ve tedavi sonrası 1, 3 ve 5. günlerde kan ve serum örnekleri alınarak biyokimyasal, hematolojik analizlerle serum akut faz proteinleri (Hp ve SAA), Total antioksidant (TAS) ve Total oksidant düzeyleri (TOS) ölçüldü. Hasta grupların her ikisinde de serum Fe, Ca konsantrasyonlarının tedavi öncesinde (0. gün) kontrol grubuna göre önemli derecede (P<0.05) düşük olduğu belirlendi. Bu parametrelerin tedaviyle birlikte klinik iyileşmeye paralel olarak düzeldiği gözlendi. Hasta grupların tedavi öncesinde total lökosit sayılarının kontrol grubuna göre önemli derecede yüksek olduğu belirlendi. Her iki tedavi grubundaki hayvanlarda tedavi öncesi serum amyloid A ve haptoglobulin düzeyleri ile TOS değerlerinin kontrol grubuna göre yüksek olduğu belirlendi (P<0.05). Sonuç olarak, sığırlarda solunum sistemi hastalıkları kompleksinde hematolojik ve biyokimyasal değişimlerin olduğu ve özellikle de akut faz proteinlerinden serum Hp ve SAA ile oksidatif stres parametrelerinden serum TOS konsantrasyonlarında önemli artışların olduğu saptandı.

Anahtar Kelimeler:

AFP, Biyokimya, BRDC, Hematoloji, Oksidatif stres

Investigations on Clinic, Haematology, Biochemistry, Oxidative Stress, Acute Phase Proteins in Infectious Respiratory Disease Complex (BRDC) in Cattle

In this study, clinical status, treatment, acute phase proteins, oxidant and antioxidant status, changes in serum biochemistry and hematological parameters in Bovine Respiratory Disease Complex (BRDC) in cattle were investigated. A total of 30 cattle were used in the study. The sick animals included in the study were divided into two groups. Group I (n = 10) given tulathromycin + flunixin-meglumine + Vitamin E and selenium combination. Group II (n = 10) given tulathromycin + flunixin-meglumine. Blood samples were collected one time from the control group (n = 10), and four times (pre-treatment and in 1, 3, 5 days of post-treatment) from the patient groups. Serum acute phase proteins (Hp and SAA), biochemical and hematological analyzes, total antioxidant (TAS) and total oxidant (TOS) levels were determined from all samples. Pre-treatment (day 0) serum Fe and Ca concentrations in both disease groups were significantly (P<0.05) lower compared to the control group. It was observed that these parameters improved parallel to clinical improvement with treatment. It was determined that the total leukocyte counts of the patient groups were significantly higher than the control group before the treatment. In both treatment groups, pre-treatment serum amyloid A and haptaglobulin levels and TOS levels were higher than the control group (P<0.05). In conclusion, it was found that significant increases occurred in serum Hp and SAA concentrations in acute phase proteins and serum TOS concentrations in oxidative stress parameters in Bovine Respiratory Disease Complex, and that there were significant hematologic and biochemical changes in this disease.

Keywords:

AFP, Biochemistry, BRDC, Haematology, Oxidative stress,

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