Sığırlarda Gebelikle İlişkili Glikoproteinlerin Tespitinde Kullanılan İki Test Kitinin Karşılaştırılması

Bu çalışmada; sığırlarda bir gebelik biyo-markırı olan gebelik ilişkili glikoproteinlerin kan plazmasında tespitine yönelik hazırlanan farklı iki ticari gebelik tanı kitinin (Sığır Gebelik Test Kiti ve Görsel Gebelik Test Kiti) erken gebelik tanısındaki etkinliklerinin karşılaştırılması amaçlandı. Bu amaçla inek (n=32) ve düvelerden (n=37) tohumlama sonrası 27-30. günde kan örneği alındı. Kan örneklerinden toplanan plazma örnekleri, Sığır Gebelik Test Kiti ve Görsel Gebelik Test Kitleriyle üretici firma talimatlarına göre analiz edildi. İnek ve düvelerin gebelik durumları kan örneklerinin alımından 10 gün sonra transrektal ultrasonografi ile tespit edildi. Gebeliklerin devamlılığı ise tohumlama sonrası 60. günde yapılan ikinci transrektal ultrasonografi ile teyit edildi. Sığır Gebelik Test Kiti ve Görsel Gebelik Test Kitlerinin sırasıyla; sensitiviteleri %80 - %80, spesiviteleri %91.2 - %85.3, pozitif prediktif değerleri %90.3 - %84.8, negatif prediktif değerleri %81.6 - %80.6, ve doğruluk oranları %85.5 - %82.6 oldu. Sonuç olarak; Sığır Gebelik Test Kiti ve Görsel Gebelik Test Kitinin inek ve düvelerde erken gebelik tanısında etkinliklerinin benzer olduğu ve rutin erken gebelik tespitinde güvenle kullanılabileceği kanısına varıldı.

Anahtar Kelimeler:

ELISA, Erken gebelik tanısı, Gebelik ilişkili glikoproteinler, Görsel gebelik test kiti, İnek

Comparison of Two Test Kits for the Detection of Pregnancy Associated Glycoproteins in Cattle

In this study, it was aimed to compare the efficiency of two commercial pregnancy test kits (Bovine Pregnancy Test Kit and Visual Pregnancy Test Kit) based on detection of pregnancy associated glycoproteins, which are pregnancy bio-markers in cattle for early pregnancy diagnosis in the blood plasma of cattle. For this purpose blood samples from dairy cows (n=32) and heifers (n=37) were collected between 27 and 30 days after insemination. Plasma samples collected from blood samples were analyzed with Bovine Pregnancy Test Kit and Visual Pregnancy Test Kit according to manufacturer’s protocols. Pregnancy status of cows and heifers was diagnosed ten days after blood samples collection by using transrectal ultrasonography. The pregnancy was confirmed by second transrectal ultrasonography at 60 days post insemination. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy rates of the Bovine Pregnancy Test Kit and Visual Pregnancy Test Kit were 80% - 80%, 91.2% - 85.3%, 90.3% - 84.8%, 81.6% - 80.6%, and 85.5% - 82.6%, respectively. As a result; it was concluded that Bovine Pregnancy Test Kit and Visual Pregnancy Test Kit had similar efficacies in early pregnancy diagnosis and could be used safely for routine early pregnancy diagnosis in cows and heifers.

Keywords:

Cattle, Early pregnancy diagnosis, ELISA, Pregnancy-associated glycoproteins, Visual pregnancy test kit,

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