Efe KURTDEDE, Berna GÜR, Yalçın DOĞAN, Kadir SEVİM, Erman GÜLENDAĞ

Klinik Bulgular Görülmeden Parvoviral Enteritis Tanısı Konulan Köpeklerde, CBC, Lipid Profili, Oksidatif Stres Biyobelirteçleri, Total Thiol, Native Thiol ve Disülfid Düzeylerinin Değerlendirilmesi

Bu çalışmada, klinik bulgular görülmeden 24-48 saat önce, dışkıda yapılan antijen testi ile parvoviral enteritis (CPV) tanısı konulan köpeklerde, tam kan hücreleri (CBC) sayıları, metabolik ve inflamasyon biyobelirteçleri ve oksidatif stres parametreleri birlikte değerlendirilerek; hastalığın durumu, sağaltım yöntemlerinin seçimi ve hastalığın prognozu hakkında etkin değerlendirmelerin yapılması amaçlandı. Çalışmada, parvoviral enteritis ile ilişkili klinik belirtilerin (durgunluk, kusma, ishal, dehidratasyon) görülmesinden 24-48 saat önceki dışkı antijen testi sonucuna göre CPV pozitif olan 30 köpek ile, 10 sağlıklı köpek değerlendirildi. CPV’li köpeklerde yapılan analizlerde, WBC (P < ,001), nötrofil (neut) (P < ,001) ve monosit (mono) (P = ,011) sayılarında anlamlı düşüşler, ve alanin aminotransferaz (ALT) ve aspartat aminotransferaz (AST) değerlerinde anlamlı artışlar (P < ,001) saptandı. Lipid metabolitlerinden yüksek dansiteli lipoprotein (HDL), kolesterol (Chl) ve trigliserit (Tg) düzeylerinde ve inflamasyon biyobelirteçlerinden C-reaktif protein (CRP), malondialdehit (MDA) ve paraoksanaz-1 (PON-1) değerlerinde anlamlı artışlar (P < ,001) belirlendi. Antioksidan düzeyinin değerlendirildiği total thiol (P = ,002) ve native thiol (P < ,001) değerlerinde anlamlı düşüşler, ve oksidasyonun değerlendirildiği disülfit düzeyinde anlamlı (P < ,001) artış saptandı. CPV'li köpeklerde klinik semptomların başlangıcından 24-48 saat önce alınan kan örneklerinde, CBC sayısında ve metabolik ve inflamasyon biyobelirteçlerinin düzeylerinde meydana gelen erken dönem değişikliklerin hastalığın şiddetine, uygulanacak tıbbi tedavilerin seçimine ve prognoza yön verebileceği sonucuna varıldı.

Anahtar Kelimeler:

Canine parvoviral enteritis, disulfide, MDA, PON-1, thiol

Evaluation of CBC, Lipid Profile, Oxidative Stress Biomarkers, Total Thiol, Native Thiol, and Disulfide Levels in Dogs Diagnosed with Parvoviral Enteritis Without Clinical Findings

In this study, whole blood cell count, metabolic and inflammatory biomarkers, and oxidative stress parameters were evaluated in dogs diagnosed with canine parvoviral enteritis by stool antigen testing performed 24-48 hours before any clinical findings arose. This study aimed to conduct effective evaluations of the status of the disease, the selection of treatment methods, and the prognosis of the disease. Thirty dogs with canine parvoviral enteritis positivity according to stool antigen test results 24-48 hours before the appearance of any clinical signs associated with parvoviral enteritis (lethargy, vomiting, diarrhea, dehydration) were evaluated together with 10 healthy dogs. In analyses of the dogs with canine parvoviral enteritis, significant decreases were found for white blood cell (P < .001), neutrophil (P < .001), and monocyte (P = .011) counts and significant increases were found for alanine aminotransferase and aspartate aminotransferase (P < .001 for both). Significant increases (P < .001) were also determined for high-density lipoprotein, cholesterol, and triglyceride levels among the studied lipid metabolites and C-reactive protein, malondialdehyde, and paraoxonase-1 among inflammatory biomarkers. Significant decreases were found for total thiol (P = .002) and native thiol (P < .001), with which antioxidant levels were evaluated, and a significant increase was found for disulfide (P < .001), with which oxidation was evaluated. It was concluded that early changes in blood samples taken 24-48 hours before the onset of clinical symptoms in dogs with CPV, as reflected by values of CBC and metabolic and inflammatory biomarkers, may provide information about the severity of the disease, the choice of medical treatments to be applied, and prognosis.

Keywords:

Canine parvoviral enteritis, disulfide, MDA, PON-1, thiol,

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