Osman Yaşar TEL, Songül ÖTKÜN, Ayfer Güllü YÜCETEPE, Oktay KESKİN

Buzağı Nasal Svap Örneklerinden Pasteurella multocida ve Mannheimia haemolytica İzolasyonunda Kromojenik Besiyerinin Etkinliğinin Araştırılması

Hastalık etkenlerinin doğru ve hızlı teşhisi, kontrol uygulamaları açısından en önemli basamaktır. Kromojenik besiyerleri hedef mikroorganizmalara özgü renkte kolonilerin meydana gelmesini sağlayan kültür ortamlarıdır. Bunlar hedefe özgü olduklarından sonuçların doğrulama ihtiyacı duymamakla birlikte, kullanım kolaylığı ve zaman tasarrufu sağlarlar. Bu çalışmada, sığırlarda solumun hastalığına neden olan Pasteurelleceae ailesinde bulunan Pasteurella multocida ve Mannheimia haemolytica etkenlerinin tespitinde, kromojenik besiyerinin (Pasteurella BRD Kit) etkinliğini değerlendirmek amaçlandı. Çalışmada solunum hastalığı belirtisi gösteren buzağılardan alınan nasal svap örnekleri kromojenik ve standart besiyerlerinde kültüre edildi. Üreyen şüpheli koloniler Pasteurella multocida ve Mannheimia haemolytica yönünden Polimeraz Zincir Reaksiyonu (PCR) ile doğrulandı. Kromojenik besiyeri ile 31 (%36,9) numune, hedef bakterilere özgü renkte koloni oluştururken, standard kültürel yöntem ile 28 (%33,3) numune hedef bakteriler yönünden pozitif olarak belirlendi. Her iki kültürel tanı yöntemiyle 26 numunenin sonuçları pozitif olarak tespit edildi. Sonuçlar geleneksel kültürel tanı ile karşılaştırıldığında %92,86 oranında uyumlu sonuç bulundu. Kromojenik besiyerinde üreyen renkli kolonilerin tamamı PCR ile de test edildi. Kromojenik besiyeri P. multocida’yı %92,86 (n:26), M. haemolytica’yı %100 (n:2) oranında aileye özgü renkte koloni meydana getirme suretiyle tespit ettiği belirlendi. Sonuç olarak, hedef etkenin pratik, hızlı ve yüksek doğrulukla teşhisinde kromojenik besiyerinin kullanımının yararlı olduğu kanısına varıldı.

Anahtar Kelimeler:

Kromojenik besiyeri, Pasteurella, sığır solunum hastalığı, kültür

Investigation of the Effectiveness of Chromogenic Media in the Isolation of Pasteurella multocida and Mannheimia haemolytica from Calf Nasal Swab Samples

Accurate and rapid diagnosis of disease agents is the most important step in terms of controlpractices. Chromogenic media are culture media that allow the formation of colonies in colorsspecific to target microorganisms. Because they are target-specific, they do not require validationof results but provide ease of use and time savings. In this study, it was aimed to evaluatethe effectiveness of a chromogenic medium (Pasteurella BDR kit) in the detection of Pasteurellamultocida and Mannheimia haemolytica agents in the Pasteurelleceae family, which cause respiratorydisease in cattle. In this study, nasal swab samples taken from calves showing symptomsof respiratory disease were cultured in chromogenic and standard media. Suspicious growingcolonies were confirmed by polymerase chain reaction for P. multocida and M. haemolytica. While31 (36.9%) samples formed colonies with the chromogenic medium in the color specific to thetarget bacteria, 28 (33.3%) samples were positively determined for the target bacteria using thestandard cultural method. The results of 26 samples were positive by both cultural diagnosismethods. When the results were compared with the traditional cultural diagnosis, agreementwas found to be 92.86%. All colored colonies grown on the chromogenic medium were also testedby polymerase chain reaction (PCR). It was determined that the chromogenic medium detected P.multocida at a rate of 92.86% (n = 26) and M. haemolytica at a rate of 100% (n = 2) by forming colonieswith a family-specific color. As a result, it was concluded that the use of chromogenic mediais beneficial in the practical, rapid, and high-accuracy diagnosis of target agents.

Keywords:

Kromojenik besiyeri, Pasteurella, sığır solunum hastalığı, kültür,

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