Sıçanlarda implantasyonda endometriyum dokusunun hücresel ve sıvısal savunma sistemi hücreleri üzerinde histokimyasal ve histometrik araştırmalar I. Hücresel savunma sistemi hücreleri

Bu çalışma, implantasyonda sıçan endometriyum dokusunun hücresel savunma sistemi hücrelerinin dağılımları ve yoğunluklarının histokimyasal ve histometrik yöntemlerle belirlenmesi amacıyla yapıldı. Çalışmada, 42 adet dişi Wistar Albino sıçan kullanıldı. Sıçanlardan alman uteruslardan hazırlanan kriyostat kesitlere, alfa naftil asetat esteraz pozitif hücreleri belirlemek için ANAE enzim boyaması yapıldı. T-lenfositlerin ve uterus doğal öldürücü hücrelerinin uNK’ların , implantasyonun ilk üç gününde desidual alan içinde gittikçe arttığı: endometriyumda ise, desidual alana göre daha az olan bu hücrelerin sayısının üçüncü günde desiduadaki hücre sayısına yakın bir oranda artış gösterdiği gözlendi. İmplantasyonun dördüncü gününden itibaren endometriyumda azalmaya başlayan bu hücrelerin sayılarında, altıncı günde yeniden şekillenen endometriyumla birlikte bir artışın olduğu görüldü. İmplantasyonda endometriyumda bulunmayan makrofajların miyometriyumda oldukça fazla sayıda olduğu tespit edildi. Bu çalışmada, endometriyum dokusunun hücresel savunma sistemi hücrelerinin implantasyonun farklı günlerine göre değişik dağılımlar ve yoğunluklar gösterdiği tespit edildi. Maternal immun tepkinin baskılanarak fötus'un canlılığını sürdürmesinde, diğer bazı faktörlerin genetik ve hormonal yanı sıra implantasyon sürecinde endometriyum dokusunun savunma sistemi hücrelerinin dağılımlarında meydana gelen değişimlerinde etkili olabileceği sonucuna varıldı

Anahtar Kelimeler:

Sıçan, Endometriyum, împlantasyon, Hücresel savunma hücreleri

Investigation of the cellular and humoral immune system cells in the endometrium tissue of the rat with histochemical staining and histometric methods during implantation I. Cellular immune system cells

This study was performed to investigate the distribution and density of the cellular immune system cells in the endometrium tissue of the rat with histochemical staining and histometric methods during implantation. Foıty-two female Wistar albino rats were used. The sections cut using a cryostat microtome were stained with the Acid a- Napthyl Acetate Esterase in order to observe ANAE positive cells. The number of lymphocytes and uterine natuıal killer cells increased in desidual area during the fırst three days of the implantation. The number of these cells in endometrium was less than that in desidual area but, they became equal at the end of third day of implantation. The number of these cells appeared less on the fourth day of implantation but with the growth of endometrium it increased again by sixth day of implantation. Macrophages that were not found in endometrium at implantation were abundantly deteeted in myometrium. In this study, the distribution and density of the cellular immune system cells in the endometrium tissue during implantation were determined. It was concluded that in the suppression of the maternal immune response to allow fetus survive, the changes in the distribution of immune system cells in endometrium during implantation may be important besides other factors such as genetically and hormonal determinants

Keywords:

Rat, Endometrium, implantation, Cellular defence celi,

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Finn CA, Pope MD: Infıltration of Neutrophil Polymorphonuclear Leukocytes into the Endometrial Stroma at the Time of Implantation of Ova and the Initiation of the Oil Desidual Celi Reaction in Mice, J. Reprod. Fert. 91: 365-369, (1991).
Croy BA, Wood W, King GJ: Evaluation of Intrauterine Immune Suppression During Pregnancy in a Species with Epitheliochorial Plasentation, J. Immunol 139(4): 1088-1095, (1987).
Yeh CJG, Bulmer JN, Hsl BL, Tian WT, Bittershaus C, îp SH: Monoclonal Antibodies to T Celi Receptor y / ö Complex React with Human Endometrial Glandular Epithelium, Placenta 11: 253-261,(1990).
Hunt JS, Manning LS, Wood GW: Macrophages in Murine Uterus are Immunosupressive, Cellular Immunology, 85: 499- 510,(1984).
Smârason AK, Gunnarsson A, Alfredson JH, Valdimarsson H: Monocytosis and Monocytic Infıltration of Desidua in Early Pregnancy, J. Clin. Immunol. 21: 1-5, (1986).
Hunt JS: Current Topic, the Role of Macrophages in the Uterine Response to Pregnancy (Revievv), Placenta 11: 467-475, (1990).
Mellanby J, Dwyer J, Hawkins C, Hitchen C: Effect of Experimental limbic on the Estrus Cycle and Reproductive Succes in Rats, Epilepsia 34(2): 220 - 227, (1991).
Kanter M, Öztaş E, Dalçık C: Sıçan, Fare ve Kobaylarda Gebeliğin İlk Gününü Tayin Etmede Vajinal Smear Yönteminin Kullanılması, Van Tıp Derg. 3(2): 112 - 116, (1996).
Welsh OA, Enders AC: Occlusion and Reformation of the Rat Uterine Lumen During Pregnancy, Ame. J. Anat.,167: 463-477, (1983).
Mueller J, Re GB, Buerki H, Keller HU, Hess M W, Cottier H: Nonspesifıc Differentiation of the T and B Lymphocytes in Mouse Lymph Activity A Criterion for Nodes, Eur. J. immun. 5: 270-274, (1975).
Zicca A, Leprini A, Cadoni A, Franzi AT, Ferrarini M Grossi CE: Ultrastructural Localization of Alpha - Naphthyl Acid Esterase in Human Tm Lymphocytes, Am. J. Pathol. 105: 40- 46,(1981).
Vassiliadou N, Bulmer JN: Characterisation of Endometrial T Lymphocyte Subpopulations in Spontaneous Early Pregnancy Loss, Human Reproduction 13(1): 44-47, (1998).
Kabavvat SE, Mostoufı-Zadeh M,Driscoll SG, Bhan A: Implantation Site in Normal Pregnancy, Am. J. Pathol. 118: 76- 84, (1985).
De M, Choudhuri R, Wood GW: Determination of the Number and Distribution of Macrophages, Lymphocytes, and Granulocytes in the Mouse Uterus from Mating Through Implantation, J. Leukocytes Biol. 50: 252-262, (1991).
Lea RG, Clark DA: Macrophages and Migratory Cells in Endometrium Relevant to Implantation, Bailliere’s Clin. Obstet. Gynae. 5(1): 25-59,(1991).
Noun A, Acker GM, Chaouat G, Antoine JC, Garabedian M: Cells Bearing Granulocyte Macrophage and T Lymphocyte Antigens in the Rat Uterus Before and During ovum Implantation, Clin. Exp. Immunol. 78: 494-498, (1989).
Peel S, Stewart IJ, Bulmer D: Experimental Evidence for the Bone Marrovv Origin of Granulated Metrial Gland Cells of the Mouse Uterus, Celi Tissue Res. 233: 647-656, (1983).
Head JR, Kresge CK, Young JD, Hiserodt JC: NKR-P1+ Cells in the Rat Uterus: Granulated Metrial Gland Cells are of the Natural Killer Celi Lineage, Biol. Reprod. 51: 509-523, (1994).
Tarachand U: Metrial Gland Structure, Origin Differentiation and Role in Pregnancy, Biol. Res. Pregnancy Perinatol. 7(1): 34- 36,(1986).
King A, Löke YW: Uterine Large Granular Lymphocytes: A Possible Role in Embryonic Implantation?, Am. J. Obstet. Gynecol. 162: 308-310, (1990).
Kachkache M, Acker GM, Chaouat G, Noun A, Garabedian M: Hormonal and Local Factors Control the Immunohistochemical Distribution of Immunocytes in the Rat Uterus Conceptus Implantation Effect of Overiectomy Fallopian Tuba Section, and Injection, Biol. Reprod. 45: 860-868, (1991).
Brandon JM: Leukocyte Distribution in the Uterus During the Preimplantasyon Period of Pregnancy and Phagocyte Recruitment to Sites of Blastocyst Attachment in Mice, J. Reprod. Fert. 98: 567-576, (1993).
Knisley KA, Weitlauf M: Compartmentalised Reactivity of M3/38 (anti Mac-2) and M3/84 (anti Mac-3) in the Uterus of Pregnant Mice, J. Reprod. Fertik 97: 521-527, (1993).
Tachi C, Tachi S: Macrophages and Implantation, Ann. N. Y. Acad. Sci. 476: 152-182, (1986).
Brandon JM: Macrophage Distribution in Desidual Tissue from Early Implantation to the Periparturent Period in Mice as Defıned by the Macrophage Differentiation Antigens F4/80, Macrosialin and the Type 3 Complement Receptor, J. Reprod. Fertik 103:6- 9,(1995).
YalçınA: Sıçanlarda ÖstrusSiklusunda Endometriyum Dokusunun Hücresel ve Humoral Savunma Sistemi Hücreleri Üzerinde Histokimyasal ve Histometrik Araştırmalar, Yüksek Lisans Tezi, Van, (1999).
Redline RW, Lu CY: Specifıc Defects in the Anti-listeral Immune Response in Discrete Regions of the Murine uterus and Placenta Account for Susceptibility to infection, J. Immunol. 140: 3947- 3955, (1998).
De M, Wood GW: Analysis of the Number and Distribution of Macrophages, Lymphocytes, and Granulocytes in the Mouse Uterus From Implantation Through Parturition, J. Leukocytes Biol. 50:381-392,(1991).