Eşref YEĞİN, İsmail ÇELİK, Fevzi POLAT, Ahmet KIZILTUNÇ, Sevinç KUŞKAY, Fehmi ODABAŞOĞLU, Nuri BAKAN

Diabetli Tavşanlarda Tırnak ve Kıl Proteinlerinin Nonenzimatik Glikozilasyonu

Kıl ve tırnak proteinlerinde hiperglisemiye bağlı olarak meydana gelen glikozillenmeyi ölçmek için 15 normal ve alloksan ile diabet oluşturulmuş 15 Albino tavşandan tırnak ve kıl örnekleri alındı. Glikozilasyon, kolorimetrik tiyobarbitürik asit metoduyla ölçüldü. Tırnak ve kıl örnekleri alındığında hemoglobin glikozilasyonunu ölçmek için her iki gruptan kan örnekleri alındı. Normal tavşanlarda glikozillenmiş hemoglobin 1.7 ± 0.49 nanomol früktozamin / mg Hb, tırnaktaki protein glikozilasyonu 1.5 ± 0.37 nanomol früktozamin / mg tırnak ve glikozillenmiş kıl protein glikozilasyonu 0.61 ± 0.06 nanomol früktozamin / mg kıl bulundu. Diyabetli tavşanlarda bu değerler daha yüksek olup : sırasıyla 3.15 ± 0.98 nanomol früktozamin / mg Hb, 3.2 ± 0.88 nanomol früktozamin / mg tırnak ve 0.69 ± 0.007 nanomol früktozamin / mg kıl olarak tespit edildi. Bu çalışmadaki bulgular, doku glikozilasyonunun uzun süreli stabil bir göstergesi olarak tırnak ve kıl proteinlerinin glikozilasyonunu belirlemenin diabetin mikrovasküler komplikasyonlarının araştırılmasında yararlı olacağını göstermektedir.

Anahtar Kelimeler:

Diabet, Glikozilasyon, Tırnak, Kıl

Nonenzymatic Glycosylation of Nail and Hair Proteins in Diabetic Rabbits

The extents of glycosylation of nail and hair proteins ıvere determined by the colorimetric thiobarbituric acid method in 15 experimentally alloxan induced diabetic and 15 nondiabetic Albino rabbits. Blood was taken from both diabetics and nondiabetics for measurement of hemoglobin glycosylation at the same time. In nondiabetics, the protein glycosylation in nail, hair and glycosylated hemoglobin were found 1.5 ± 0.37 nmol fructosamine per mg nail, 0,61 ± 0.06 nmol fructosamine per mg hair and 1.7 ± 0.49 nmol fructosamine per mg Hb. In the diabetic group there ıvas an extremely high glycosylation in nail, hair proteins and hemoglobin: 3.2 ± 0.88 nmol fructosamine per mg nail, 0.69 ± 0.007 nmol fructosamine per mg hair and 3.15 ± 0.98 nmol fructosamine per mg Hb. Glycosylation of nail, hair and hemoglobin might provide a stable long term measure of tissue glycosylation, useful in the investigation of diabetic microvascular complications.

Keywords:

Diabetes, Glycosylation, Nail, Hair,

PDF

___

Jovanovic L and Peterson CM : The clinical utility of glycosylated hemoglobin. J Am Med. 70: 331, 1981.
Mc Donalt JM and Davis JR : Glycosylated hemoglobins and diabetes mellitus. Human Pathol. 10 : 279, 1981.
Goner B and Rubenstein AH : Hemoglobin Aj and diabetes mellitus. Diabetologia. 15 : 1, 1978.
Bunn HF, Gabbay KH and Gallop PM : The glycosy- lation of Hb : Relevance to diabetes mellitus. Science. 200 : 21, 1978.
Seven A : Increased glycation of hair and nail proteins in patients with diabetes. Türk J Med Biol Res. 4 : 140, 1993.
Bakan E, Bakan N ve Ağbaş A : Diabetli hastalarda tırnak proteinlerinin glikozilasyonu. C 10 : 8, 1986.
Sueki H : Glycosylated proteins of skin, nail and hair : Application as an mdex for long-term control of diabetes mellitus. J Dermatol. 16 : 103, 1989.
Seven A : Correlation between dıabetic neuropathy and glycated hemoglobin, hairglycation and blood sorbitol. MedSciRes. 21 : 161, 1993.
Seven A and Candan G : Advanced glycation endpro- ducts and diabetes mellitus. Türk J Med Biol Res. 3 : 225, 1992.
Rakhimova GN, Akbarov ZS and Turakulov IaKII : Determining the time of diabetes mellitus onset by the level of glycosylated keratin in hair. Probl Endokrinol. 38 (3): 21, 1992.
Parker KM, England JD and Da Cosfta J : Improved colorimetric assay for glycosylated hemoglobin. Clin Chem. 27:669,1981.
Oimomi M : Increased fructose-Lysine of nail protein in diabetic patients. Klin Vvochenschr. 62 (10): 477, 1984.
PaiseyRB, Clamp JR and Kent MJC : Glycosylatıon of hair : Possible marker of chronic hvperglycemia. British MedJ. 288: 669, 1984.
Oimomi M, Nishimotoa S and Kitamura Y : Increased fructose-lysine of nail protein and blood glucose control ııı diabetic patients. Horm Metab Res 18: 827, 1986.
Oimomi M and Igaki N : Hair protein glycation as a long-term. index of blood glucose in diabetics. Diabetes Res and Clin Pract. 5 : 305, 1988.