SERAN TEMELLİ, Cem ŞEN, Saltan Süreyya EVRENSEL, Nur YÜKSEK

Soğuk olarak tüketime sunulan bazı hazır gıdaların mikrobiyolojik kalitelerinin incelenmesi

Çalışmada, toplam 60 adet soğuk olarak tüketime sunulan hazır gıda Örneği (Rus salata, ttalyan salata, Danish salata, tavuk salata, kadınbudu köfte ve arnavut ciğeri) mikrobiyolojik olarak toplam aerobik mezofilik bakteri, koliform bakteriler, E. coli, Enterobacteriaceae, enterokoklar, stafılokoklar, koagülaz pozitif stafilokoklar, maya ve küf, sülfıt indirgeyen anaerob bakteriler ve Salmonella spp. yönünden incelenmiştir. Analizler sonucunda, ömek türlerine bağlı olarak toplam aerobik mezofilik bakteri sayısı İO^-IO7 kob/g, koliform bakteriler 10M05 kob/g, Enterobacteriaceae İOMO4 kob/g, enterokoklar İOMO4 kob/g, stafilokoklar 10M05 kob/g, maya ve küf sayısı da 103-104 kob/g düzeyleri arasında saptanmıştır. Ayrıca, incelenen örneklerde sülfıt indirgeyen anaerob bakterilerin sayısının saptama sınırının altında olduğu tespit edilmiş, Salmonella spp. varlığına rastlanılmamıştır. Sonuç olarak, soğuk tüketime sunulan hazır gıdaların mikrobiyolojik kalitesinin düşük ve değişen oranlarda E. coli ve koagülaz pozitif stafılokokları içermesi nedeniyle halk sağlığı yönünden riskli olduğu belirlenmiştir.

Anahtar Kelimeler:

anaeroblar, gıda bulaşıklılığı, gıda kalitesi, mikrobiyal bulaşıklık, küfler, mayalar, salatalar, Enterobacteriaceae, Enterococcus, Escherichia coli, Salmonella, Staphylococcus, koliform bakterileri

Assessment of microbiological quality of cold served ready to eat foods

In this study, a total of 60 cold served ready to eat food samples (Russian salad, Italian salad, Danish salad, chicken salad, kadınbudu meat balls and Albanian sytyle liver) were examined for the presence of lotal aerobic mesophilic bacteria, coliforms, E. coli, Enterobacteriaceae, enterococci, staphylococci, coagulase positive staphylococci, yeast and mold, sulfite reducing anaerobs and Salmonella spp. Analyses revealed the following counts: total aerobic mesophilic bacteria 105-107 cfu/g, coliforms İO'-IO5 cfu/g, Enterobacteriaceae lO'-lO4 cfu/g, enterococci 103-104 cfu/g, staphylococci 103-105 cfu/g, yeast and mold 103-104 cfu/g. Counts for the sulfite reducing anaerobs were under the detection limit, and no Salmonella spp. was isolated. These results indicate that the microbiological status of cold served ready to eat food samples were not high and the presence of E. coli and coagulase positive staphylococci in the samples analysed show thai these foods pose risk for public health.

Keywords:

anaerobes, food contamination, food quality, microbial contamination, moulds, yeasts, salads, Enterobacteriaceae, Enterococcus, Escherichia coli, Salmonella, Staphylococcus, coliform bacteria,

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AKSU H. İstanbul'da Tüketime Sunulan Bazı Hazır Yemeklerin Mikrobiyolojik Kalitesi Üzerine Araştırmalar. V. Ulusal Halk Sağlığı Kongresi. 12- 16 Ekim 1996, İstanbul.

AYÇİÇEK H, SARIMEHMETOĞLU B, ÇAKKOĞLU S. Assessment of (he Microbiological Quality of Meals Sampled at the Meal Serving Units of a Military Hospital in Ankara, Turkey. Fcxtd Conirol 2004; 15: 379-384.

BAŞOĞLU F. Gıdalarda Kullanılan Bazı Baharatların Mikroorganizmalar Üzerine Etkileri ve Koııtaminasyondaki Rolleri. Gıda 1982:7: 19-24.

BHARATHI S, RAMESH MN, VARADARAI MC. Predicting the Behavioural Pattern of Escherichia colt in Minimally Processed Vegetables. Food Control 2001; 12: 275-284.

DROSINOS EH, GIALITAKI M, PARAMITHIOTIS S, METAXOPOULOS J. A Survey of the Microbiological Quality of Some Food Catering Services in Greece. Ital J Food Sci 2005; 17:469-476.

ELEFTHERIADOU M, VARNAVA-TELLO A, METTA-LOIZEDOU M, NIKOLAOU AS, AKKELIDOU D. The Microbiological Profile of Foods İn the Republic of Cyprus: 1991-2000. Food Microbiol 2002; 19: 463-471.

FANG TJ, WEI Q, LIAO C, HUNG M, WANG T. Microbiological Quality of 18°C Ready-to-Eat Food Products Sold in Taiwan. Int J Food Microbiol 2002; 24: 1-10.

GIBBONS I, ADESIYUN A, SEEPERSADSINGH N, RAHAMAN S. Investigation for Possible Source(s) of Contamination of Ready-to-Eat Meat Products with Listeria spp. and other Pathogens in a Meat Processing Plant in Trinidad. Food Microbiol 2006; Baskıda.

GÖKTAN D, TUNCEL G. İzmir'de Satılan Rus Salatalarının Hijyenik Durumu. Ege Üniversitesi Mühendislik Fakültesi Dergisi 1984; 12: 21-28.

GÖNÜL ŞA, KARAPINAR M, KARAGÖZLÜ N. Piyasada Satılan Meze Tipi Soğuk Yiyeceklerin Mikrobiyolojik Kaliteleri. Turk J Biol 1996; 20: 263-271.

ICMSF. International Commission on Microbiological Specifications for Foods. Microorganisms in Foods. Their Significance and Methods of Enumeration. 2nd ed. University of Toronto Press, 1982.

KANEKO K, HAYASHIDANI H, TAKAHASHI K, SHTRAKI Y, LIMAWONGPRANEE S, OGAWA M. Bacterial Contamination in the Environment of Food Factories Processing Ready-to-Eat Fresh Vegetables. J Food Prot 1999; 62: 800-804.

KANEKO K, HAYASHIDANI H, OHTOMO Y, KOSUGE J, KATO M, TAKAHASKI K, SHIRAKI Y, OGAWA M. Bacterial Contamination of Ready-to-Eai Foods and Fresh Products in Retail Shops and Food Factories. J Food Prot 1999; 53: 644-649.

KUBHEKA LC, MOSUPYE FM. von HOLY A. Microbiological Survey of Sireet Vended Salad and Gravy in Johannesburg City, South Africa. Food Control 2O01;12: 127-131.

LITTLE CL, LOUVOIS J, ROBERTS D, YOUNGS E. Microbiological Quality of Retail Imported Unprepared Whole Lettuces: A PHLS Food Working Group Study. J Food Prot 1999; 62: 325-328.

LITTLE CL, OMOTOYE R, MITCHELL RT. The Microbiological Quality of Ready-to-Eat Foods with Added Spices. Int J Environ Health Res 2003; 13:31-42.

Mc NAMARA AM, MAGEAN RP, GREEN SS. Microbiology Laboratory Guidebook. United States Department of Agriculture Food Safety and Inspection Service Office of Public Health and Science Microbiology Division. 3rd ed., 1998.

MOSUPYE FM, von HOLY A. Microbiological Hazard Identification and Exposure Assesment of Street Food Vending in Johannesburg, South Africa. Int J Food Microbiol 2000; 61: 137-145.

ÖNER E. Soğuk Olarak Tüketime Sunulan Bazı Hazır Ticari Yiyeceklerin Mikrobiyolojik Kalitelerinin Belirlenmesi (Yüksek Lisans Tezi). Ankara Üniversitesi Sağlık Bilimleri Enstitüsü, Ankara, 1997.

PATRICIA MA, AZANZA V. Aerobic Plate Counts of Philippine Ready-to-Eat Foods from Take-Away Premises. J Food Safety 2005; 25: 80-97.

PINGULKAR K, KAMAT A, BONGIRWAR D. Microbiological Quality of Fresh Leafy Vegetables, Salad Components and Ready-to-Eat Salads; an Evidence of Inhibition of Listeria monocytogenes in Tomatoes. Int J Food Sci Nutr 2001; 52: 15-23.

SAGOO SK, LITTLE CL, WARD L, GILLESPIE IA, MITCHELL RT. Microbiological Study of Ready-to-Eat Salad Vegetables from Retail Establishments Uncovers a National Outbreak of Salmonellosis. J Food Prot 1999; 66: 403-409.

SORIANO JM, RICO H, MOLTO JC, MANES J. Incidence of Microbial Flora in Lettuce, Meat and Spanish Potato Omelette from Restaurants. Food Microbiol 2001;18: 159-163.

TOURNAS VH. Moulds and Yeasts in fresh and Minimally Processed Vegetables, and Sprouts. Int J Food Microbiol 2005; 99: 71-77.

WONG TL, CAREY-SMITH GV, HOLLIS L, HUDSON JA. Microbiological Survey of Prepackaged Pate and Ham in New Zealand. Lett Appl Microbiol 2005; 41: 106-1II.