Pavel HYRL, Ender BÜYÜKGÜZEL, Kemal BÜYÜKGÜZEL

Eikosanoid biyosentezi inhibitörlerinin Galleria mellonella (Linnaeus) larvalarının hemolenf protein profiline etkisi

Anahtar Kelimeler:

Eikosanoidler, eskuletin, Galleria mellonella, hemolenf, protein profili

Effect of eicosanoid biosynthesis inhibitors on the haemolymph protein profile of Galleria mellonella (Linnaeus) larvae

Eicosanoids mediate insects cellular and humoral immune reactions and stress responses. Function of these mediators can be specifically blocked using different eicosanoid biosynthesis inhibitors (EBIs). Effects of EBIs on total haemolymph protein composition have not been extensively studied in insects. We posed the hypothesis that eicosanoids also mediate physiological homeostasis by regulating protein profiles involved in stress response and other defensive reactions. To test this idea,we reared greater wax moth Galleria mellonella (Linnaeus,1758) (Lepidoptera: Pyralidae) larvae on artificial diets containing 0.001, 0.01, 0.1 or 1.0% of specific EBIs with different mode of action: Esculetin, dexamethasone and phenidone. Feeding larvae with esculetin caused significantly dose-dependent changes in 45 kDa protein fraction (one of 16 proteins detected) using sodium dodecyl-sulphate polyacrylamide gradient gel electrophoresis followed by silver staining. Other main haemolymph proteins, lipophorins (ApoLP-I) and storage proteins, were not affected by EBIs treatments. Dexamethasone and phenidone caused no significant differences in detected protein fractions. We infer from these findings that eicosanoids, at least lipoxygenase products, have been implicated in the protein composition of insect tissues as structural and functional concept. Although it has not yet been possible to use directly EBIs for insect pest control, our results bring new data to understand physiological signaling systems in insects

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