Ender BÜYÜKGÜZEL, Kemal BÜYÜKGÜZEL, Pavel HYRSL

Effect of eicosanoid biosynthesis inhibitors on the haemolymph protein profile of Galleria mellonella (Linnaeus, 1758) larvae (Lepidoptera: Pyralidae)

Eikosanoidlerin böceklerin hücresel, humoral immün savunma reaksiyonlarına ve strese karşı tepkilerine aracılık ettiği bilinmektedir. Bu aracı moleküllerin işlevi farklı eikosanoid biyosentezi inhibitörleri tarafından spesifik olarak önlenebilir. Bu inhibitörlerin böceklerde total hemolenf protein bileşimi üzerine etkileri detaylı olarak çalışılmamıştır. Eikosanoidlerin böceklerde stress faktörlerine karşı sentezlenen ve diğer savunma reaksiyonlarından sorumlu proteinler üzerinde etkili olarak fizyolojik dengenin sağlanma-sına aracılık ettiği düşünülmüştür. Bu sebeple Büyük bal mumu güvesi Galleria mellonella (Linnaeus, 1758) (Lepidoptera: Pyralidae) larvaları farklı etki mekanizmalarına sahip eikosanoid biyosentezi inhibitörleri eskuletin, deksametazon ve fenidonu % 0.001, 0.01, 0.1 ve 1.0 oranında içeren yapay besinler ile beslenmiştir. Larvaların eskuletin ile beslenmesi 45 kDa protein bileşiminde (belirlenen 16 protein bandından biri) uygulanan inhibitörün dozuna bağlı olarak önemli değişime sebep olmuştur. Diğer başlıca hemolenf proteinlerinden lipoforinler (ApoLP-I) ve depo proteinleri üzerinde denenen eikosanoid biyosentezi inhibitörlerinin önemli bir etkisi olmamıştır. Deksametazon ve fenidon hemolenf proteinlerinde önemli bir değişikliğe sebep olmamıştır. Bu çalışmanın sonuçları eikosanoidlerin, en azından lipoksijenaz ürünlerinin, böcek dokularında yapısal ve işlevsel olarak protein bileşimi üzerinde etkili olduğunu göstermiştir. Eikosanoid biyosentezi inhibitörlerinin doğrudan zararlı böceklerin kontrolünde kullanılması yaygın olmasa da bu çalışmadan elde edilen sonuçlar böceklerde eikosanoidlerin fizyolojik sinyal sistemlerindeki rollerinin anlaşılmasına katkıda bulunmaktadır.

Eikosanoid biyosentezi inhibitörlerinin Galleria mellonella (Linnaeus, 1758) (Lepidoptera: Pyralidae) larvalarının hemolenf protein profiline etkisi

Eicosanoids mediate insects cellular and humoral immune reactions and stress responses. Function of these mediators can be specifically blocked using different eicosanoid biosynthesis inhibitors (EBIs). Effects of EBIs on total haemolymph protein composition have not been extensively studied in insects. We posed the hypothesis that eicosanoids also mediate physiological homeostasis by regulating protein profiles involved in stress response and other defensive reactions. To test this idea, we reared greater wax moth Galleria mellonella (Linnaeus,1758) (Lepidoptera: Pyralidae) larvae on artificial diets containing 0.001, 0.01, 0.1 or 1.0% of specific EBIs with different mode of action: Esculetin, dexamethasone and phenidone. Feeding larvae with esculetin caused significantly dose-dependent changes in 45 kDa protein fraction (one of 16 proteins detected) using sodium dodecyl-sulphate polyacrylamide gradient gel electrophoresis followed by silver staining. Other main haemolymph proteins, lipophorins (ApoLP-I) and storage proteins, were not affected by EBIs treatments. Dexamethasone and phenidone caused no significant differences in detected protein fractions. We infer from these findings that eicosanoids, at least lipoxygenase products, have been implicated in the protein composition of insect tissues as structural and functional concept. Although it has not yet been possible to use directly EBIs for insect pest control, our results bring new data to understand physiological signaling systems in insects.

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