Mikail AKBULUT, Abdurrahman AYVAZ, Semih YILMAZ, Sabir SADYKHOV, Recep ERÖZ

Age dependent DNase activity in larvae, pupae and adult stages of Mediterranean Flour Moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae)

Programlanmış hücre ölümü normal embriyonik gelişimde, ergin dokularının korunmasında, hücre sayısının kontrol edilmesi suretiyle hayvan homeostazisinin sürdürülmesinde anormal hücrelerin uzaklaştırılmasında, normal gelişim sırasında gelişim dönemlerinin şekillenmesinde önemli bir süreçtir. DNaz etkisiyle oluşturulan DNA parçalanması, böcek gelişimi ve morfogenezindeki önemli olaylardan biridir. Bu çalışmada holometabol başkalaşım gösteren Ephestia kuehniella Zeller (Pyralidae: Lepidoptera)’nın gelişim dönemlerindeki DNaz aktivitesi incelenmiştir. Larvadan pupaya geçiş sırasında E. kuehniella’nın dokuları yıkıma uğrar. 27 günlük larva, 1 günlük pupa ve genç erginlerin (1 günlük) DNaz aktivitesi sırasıyla 0.270, 0.428 ve 0.310 kunitz unit (Ku) olarak bulunmuştur. Larva, pupa ve erginlerin orta yaş grupları için DNaz aktivitesi sırasıyla 0.376, 0.759 ve 0.436 Ku olarak bulunmuşken ileri yaş dönemleri için 0.522, 1.535 ve 1,156. Ku olarak hesaplanmıştır. Larva, pupa ve ergin dönemlerinde yaşa bağlı olarak DNaz aktivitesinin arttığı gözlenmiştir. Elde edilen bulgular başkalaşım sırasında DNaz aktivitesinin programlanmış hücre ölümüne neden olan başlıca etkenlerden biri olduğunu göstermiştir. DNaz aktivitesinin en fazla pupa döneminde, en az ise larva döneminde olduğu belirlenmiştir.

Un güvesi Ephestia kuehniella Zeller (Lepidoptera: Pyralidae)’nın larva, pupa ve ergin dönemlerindeki yaşa bağlı DNaz aktivitesi

Programmed cell death is an important process in normal embryonic development, adult tissue maintenance, maintaining animal homeostasis by controlling cell numbers, removing abnormal cells, and sculpting developmental structures in normal morphogenesis. DNA fragmentation, carried out by DNase activity, is one of the important events during morphogenesis of insect development. In this study, DNase activities of holometabolic Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) were evaluated during its life stages. The tissues of E. kuehniella degenerate during larval-pupal metamorphosis. The DNase activities for the 27 day old larvae, 1 day old pupae and adult (youngest individuals) were found to be 0.270, 0.428, and 0.310 kunitz unit (Ku), respectively. The DNase activities of larvae, pupae and adult were found to be 0.376, 0.759 and 0.436 Ku for middle; and 0.522, 1.535 and 1.156 Ku for the oldest age groups, respectively. There was increasing DNase activity of larvae depending on the ages. Age dependent increase in DNase activity was also observed for pupae and adults. Our findings indicated that DNase activity is the leading force for programmed cell death during metamorphosis. When different developmental stages were considered, the highest DNase activity was observed in pupae and the lowest in the larvae.

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