Seasonal changes in fatty acid composition of Eysarcoris inconspicuous (Herrich-Schaffer, 1844) (Heteroptera: Pentatomidae) adults

Bu çalışmanın amacı, Eysarcoris inconspicuous (Herrich-Schaffer, 1844) (Heteroptera: Pentatomidae) erginlerinin fosfolipit ve triaçilgliseroldeki yağ asidi kompozisyonlarda mevsimsel farklılıklar sonucu meydana gelen değişimi araştırmaktır. E. inconspicuous erginleri Diyarbakır’dan (Türkiye) 2007-2008 yılları arasında toplanmıştır. Fosfolipit ve triaçilgliserol fraksiyonlarındaki yağ asidi kompozisyonları E. inconspicuous’un tüm vücudu kullananılarak izole edilmiş ve gaz kromatografisi ve gaz kromatografi-kütle spektrometresi ile analizlenmiştir. Ayların çoğunluğunda kalitatif olarak 15 yağ asidinin varlığı ortaya çıkarılmıştır. Majör olan yağ asitleri çoğu hayvanlarda da gözlenen 16 ve 18 karbonlu doymuş ve doymamış bileşenlerdir. Bu bileşenlere ek olarak, üç adet tek zincirli (C13:0), (C15:0), (C17:0) ve prostaglandinlerin öncül maddesi olan yağ asitleri de bulunmuştur. Fosfolipit ve triaçilgliserol yağ asidi profillerinde bazı farklılıklar gözlenmiştir. Triaçilgliserolün aksine fosfolipitte linolenik asit ve 20 karbonlu aşırı doymamış yağ asitlerinde sonbahar ve kış süresince artış gözlenmiştir. Her iki fraksiyonda da aşırı doymamış yağ asitlerinin oranı doymuş yağ asitlerine göre önemli derecede artış göstermesine rağmen bu artış sonbaharda ve özelikle mevsim sıcaklığının düşük olduğu ocak ve şubat aylarında fosfolipitte kendisini bariz olarak hissetirmiştir. Bu nedenle E. inconspicuous’un yağ metabolizmasında mevsimsel farklılıktaki sıcaklık değişiminin önemli bir rol oynadığı görülmektedir. Böcekler için düşük sıcaklıktan dolayı meydana gelen hücresel hasarı önlemek önemli bir sorundur. Bu bulgularımız, E. inconspicuous’un çevresel şartların değişiminden dolayı kendi yağ asidi kompozisyonunu modifiye edebildiğini göstermektedir.

Eysarcoris inconspicuous (Herrich-Schaffer, 1844) (Heteroptera: Pentatomidae) erginlerinin yağ asidi kompozisyonundaki mevsimsel değişimler

The goal of the study was to investigate the role of phospholipid and triacylglycerol fatty acid compositional changes in Eysarcoris inconspicuous (Herrich-Schaffer, 1844) (Heteroptera: Pentatomidae) with respect to seasonal changes. E. inconspicuous adults were collected from Diyarbakır, Turkey in 2007-2008. The fatty acid compositions of phospholipid and triacylglycerol fractions that were extracted from whole-body of adult E. inconspicuous were isolated and analyzed using gas chromatography and gas chromatography-mass spectrometry. Qualitative analysis has revealed the presence of 15 fatty acids during most of the months. The major components were C16 and C18 saturated and unsaturated components which are ubiquitous in most animal species. In addition to these components, three odd-chain (C13:0), (C15:0), (C17:0), and prostaglandin precursor fatty acids were found. The fatty acid profiles of phospholipids and triacylglycerols have some diferences. In contrast to triacylglycerol fraction, linolenic acid and C20 polyunsaturated fatty acids increased during autumn and winter in phospholipid fraction were detected. The unsaturated fatty acid to saturated fatty acid ratio significantly increased in both fractions but the increase was dramatic in phospholipid fraction during autumn, and reaches its maximum level in january and february, when outdoor temperatures are low. Thus, temperature seems to play an important role in seasonal variation of lipid metabolism of E. inconspicuous. Preventing cellular damage due to low temperatures is a major challenge for insects. These findings indicate that E. inconspicuous can modify its fatty acid composition in response to changes in environmental conditions.

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