Yaygın olarak kullanılan bazı biyopestisitlerin yerel entomopatojen nematod izolatlarının hayatta kalma ve virülensliği üzerindeki etkileri

Bazı yerel entomopatojen nematod (EPN) izolatlarının [Heterorhabditis bacteriophora FLH-4H (Poinar, 1975) (Rhabditida: Heterorhabditidae), Steinernema carpocapsae KCS-4S (Weiser, 1955) (Rhabditida: Steinernematidae) ve Steinernema feltiae KMP-9S (Filipjev, 1934) (Rhabditida: Steinernematidae)] bazı biyopestisitlerle (40 g/ L Azadirachtin, 480 g/ L Spinosad) olan uyumluluğu laboratuvar şartları altında incelenmiştir. Bu biyopestisitlerin, EPN izolatlarına ait infektiv juvenillerin (IJ) canlılığı ve virülensliği üzerindeki etkisi, IJ’leri biyopestisitlerin önerilen en yüksek arazi konsantrasyonlarına 24 ve 48 saat boyunca maruz bırakılarak incelenmiştir. Biyopestisitlere 24 ve 48 saatlik sürelerde maruz kalan EPN izolatlarının hayatta kalma oranları % 5-21 arasında değişmiştir. Heterorhabditis bacteriophora FLH-4H izolatına ait IJ’lerin ölüm oranı her iki uygulama süresinde de %12’yi geçmemiştir ve test edilen biyopestisitlere en tolerant izolat olduğu belirlenmiştir. Test edilen biyopestisitler, EPN'ların virülensliğini önemli düzeyde etkilememiştir ve Galleria mellonella L. (Lepidoptera: Pyralidae) larvalarının ölüm oranları % 70-95 arasında değişmiştir.

Anahtar Kelimeler:

Entomopatojen nematod, Steinernema sp., Heterorhabditis sp., Azadirachtin, Spinosad

Effects of some commonly used biopesticides on the survival and virulence of native entomopathogenic nematode isolates

The compatibility of the use of native Turkish entomopathogenic nematode (EPN) isolates, Heterorhabditis bacteriophora FLH-4H (Poinar, 1975) (Rhabditida: Heterorhabditidae), Steinernema carpocapsae KCS-4S (Weiser, 1955) (Rhabditida: Steinernematidae) and Steinernema feltiae KMP-9S (Filipjev, 1934), with two biopesticides, 40 g/ L Azadirachtin and 480 g/ L Spinosad, was investigated under laboratory conditions. The effects of these biopesticides on the viability and virulence of IJs of EPN isolates was studied by direct exposure to the biopesticides for 24 h and 48 h at the highest concentrations recommended for field application. Survival rates of the EPN isolates ranged between 5% and 21%. Heterorhabditis bacteriophora FLH-4H was the most tolerant isolate, with mortality of IJs not exceeding 12% for both exposure times. The biopesticides did not affect the virulence of the EPNs, with the mortality of Galleria mellonella L. (Lepidoptera: Pyralidae) larvae ranging between 70% and 95%.

Keywords:

Entomopathogenic nematodes, Steinernema sp., Heterorhabditis, Azadirachtin, Spinosad,

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