Pirinç Biti, Sitophilus Oryzae (L.), (Curculionidae: Coleoptera)'ye karşı bazı yerel Steinernema feltiae izolatlarının laboratuar koşullarında değerlendirmesi
Üç yerel entomopatojen nematod (EPN) izolatının (Steinernema feltiae UKK-1, S. feltiae DTK-6 ve S. feltiae UIP-19) Sitophilus oryzae (L.), (Curculionidae: Coleoptera) erginleri üzerindeki etkinliği farklı konsantrasyonlarda (50, 100, 250, 500, 1000 ve 2000 IJs /ergin) laboratuvar koşullarında (25oC ve %65 RH) incelenmiştir. Sitophilus oryzae erginlerinin ölüm oranları uygulama sonrası 4. ve 8. günlerde kaydedilmiştir. Ölüm oranları artan uygulama konsantrasyonlarıyla birlikte artmıştır ve ölüm oranları %7-92 arasında değişmiştir. Uygulama sonrası 4. ve 8. gündeki en yüksek ölüm oranları sırasıyla %80 ve %92 olarak gerçekleşmiştir. Laboratuvar çalışmaları test edilen EPN izolatlarının S. oryzae’nin mücadelesinde iyi bir potansiyele sahip olduğunu göstermektedir.
Laboratory evaluation of some native isolates of Steinernema feltiae against the Rice Weevil, Sitophilus Oryzae (L.), (Curculionidae: Coleoptera)
The efficacy of three native entomopathogenic nematode (EPN) isolates Steinernema feltiae UKK-1, S. feltiae DTK-6, and S. feltiae UIP-19) on the adults of Sitophilus oryzae (L.), (Curculionidae: Coleoptera) was investigated at different concentrations (50, 100, 250, 500, 1000 and 2000 IJs/adult) in laboratory bioassays (25oC and 65% RH). Mortality rates of S. oryzae were evaluated after 4th and 8th day of exposure time. Mortality rates increased with the increasing the concentrations and ranged between 7% and 92%. The highest mortality rates were 80% and 92% at the 4th and 8th days after treatment, respectively. The results from the laboratory tests showed that EPN isolates tested have a good potential for the management of S. oryzae.
___
- Ehlers, R. U., 2001. Mass production of entomopathogenic nematodes for plant protection. Applied microbiology and biotechnology, 56(5-6):623-633.
- Fragoso D. B., Guedes R. N. C. & S. T. Rezende, 2003. Glutathione S‐transferase detoxification as a potential pyrethroid resistance mechanism in the maize weevil, Sitophilus zeamais. Entomologia Experimentalis et Applicata, 109(1): 21-29.
- Kaya H. K. & R. Gaugler, 1993. Entomopathogenic nematodes. Annual review of entomology, 38(1): 181-206.
- Kaya H. K. & S. P. Stock, 1997. Techniques in insect nematology (Editor: L.A. Lacey, Manual of techniques in insect pathology). Academic Press, California, 281-324.
- Laznik Ž., Tóth T., Lakatos T., Vidrih M. & S. Trdan, 2010. The activity of three new strains of Steinernema feltiae against adults of Sitophilus oryzae under laboratory conditions. Journal of Food, Agriculture and Environment, 8(1): 150-154.
- Pimentel M. A. G., Faroni L. R. D. A., Tótola M. R. & R. N. C. Guedes, 2007. Phosphine resistance, respiration rate and fitness consequences in stored‐product insects. Pest Management Science, 63(9): 876-881.
- Shaaya, E., Kostjukovski, M., Eilberg, J. & C. Sukprakarn, 1997. Plant oils as fumigants and contact insecticides for the control of stored-product insects. Journal of Stored Products Research, 33(1): 7-15.
- Shahina F. & Salma, J. 2010. Laboratory evaluation of seven Pakistani strains of entomopathogenic nematode against stored grain insect pest Sitophilus oryzae L. Pakistan Journal of Nematology, 28(2): 295-305.
- Trdan S., Vidrih M. & N. Valic, 2006. Activity of four entomopathogenic nematode species against young adults of Sitophilus granarius (Coleoptera: Curculionidae) and Oryzaephilus surinamensis (Coleoptera: Silvanidae) under laboratory conditions. Journal of plant diseases and protection, 113(4): 168-173.
- Trdan S., Vidrih M., Andjus L. & Ž. Laznik, 2009. Activity of four entomopathogenic nematode species against different developmental stages of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera, Chrysomelidae). Helminthologia, 46(1): 14-20.
- Yüksel E., Açıkgöz Ç., Demirci F., & M. Muștu 2017. Effects of the entomopathogenic fungi, Beauveria bassiana, Isaria farinosa and Purpureocillium lilacinum, on eggs of Tuta absoluta (Meyrick)(Lepidoptera: Gelechiidae). Türkiye Biyolojik Mücadele Dergisi, 8(1): 39-47.
- Yuksel, E., & R. Canhilal, 2019. Isolation, İdentification, And Pathogenicity Of Entomopathogenic Nematodes Occurring İn Cappadocia Region, Central Turkey. Egyptian Journal Of Biological Pest Control, 29(1): 40.