Türkistan hamamböceği Blatta lateralis’in Türk entomopatojen nematod izolatlarına olan hassasiyetinin belirlenmesi

Bu çalışmada iki yerel entomopatojen nematod izolatının (Steinernema feltiae-KV06 (Filipjev) (Rhabditida: Steinernematidae) ve Heterorhabditis bacteriophora-EO7 (Rhabditida: Heterorhabtidae) Blatta lateralis Walker (Blattodea: Blattidae)’in biyolojik mücadelesindeki kullanım olanakları araştırılmıştır. Çalışmada kullanılan izolatlar Ankara ili Kelebekler Vadisi ve Eğriova Tabiat Parklarından elde edilmiştir. İzolatlar, 9cm çapındaki petri kutularına bırakılan B. lateralis nimflerine 100, 250, 500 ve 1000 IJ/cm2 dozlarında uygulanmıştır. B. lateralis nimflerindeki ölümler, uygulamadan 2, 4, 6, 8, 10, 12 ve 14gün sonra kaydedilmiştir. 1000 IJ/cm2'de, S. feltiae-KV06 ve H. bacteriophora-EO7’nın, LT50 değerleri 2.3 ve 2.6 gün ve LT90 değerleri ile sırasıyla 6.6 ve 7.9 gün ile en yüksek ölüm oranına (% 100.0 ± 0.0) neden olmuştur. Her iki izolattaki en düşük ölüm yüzdesi, 2 gün maruziyetten sonra 100IJs/cm2'de belirlenmiştir (S. feltiae-KV06: 0.6±1.4 ve H. bacteriophora-EO7: 0.0±0.0). Farklı zaman aralıklarındaki LC50,90 değerleri her iki izolat arasında gözardı edilebilecek düzeyde farklılık göstermekle birlikte, S. feltiae-KV06’nın B. lateralis nimflerine karşı H. bacteriophora-EO7’dan daha etkili olduğu görülmektedir. Bununla birlikte, her iki izolatın da farklı sıcaklık, nem ve beton, parke ve fayans gibi farklı yüzeylerde de zararlı üzerindeki etkinliklerinin test edilmesi gerekmektedir.

Anahtar Kelimeler:

entomopatojen nematod, biyolojik mücadele, Blatta lateralis

Assessment of the susceptibility of the Turkestan cockroach, Blatta lateralis to Turkish isolates of entomopathogenic nematodes

Two Turkish entomopathogenic nematodes isolates, Steinernema feltiae-KV06 (Filipjev) (Rhabditida: Steinernematidae) and Heterorhabditis bacteriophora-EO7 (Rhabditida: Heterorhabtidae), were tested against the cockroach, Blatta lateralis Walker (Blattodea: Blattidae) to determine their potential for its control. The isolates were obtained from Kelebekler Vadisi and Eğriova Nature Parks in Ankara Province, Turkey. These isolates were applied at concentrations of 100, 250, 500 and 1000 IJ/cm2 to B. lateralis nymphs in 9cm diameter Petri dishes. The mortalities were recorded at 2, 4, 6, 8, 10, 12 and 14 days after application. At 1000 IJs/cm2, S. feltiae-KV06 and H. bacteriophora-EO7 caused the highest mortality (100.0±0.0%) with an LT50 of 2.3 and 2.6 days, respectively, and an LT90 of 6.6 and 7.9 days, respectively. The lowest mortality rate caused by both species was at 100IJs/cm2 after 2 days of exposure (S. feltiae-KV06: 0.6±1.4; H. bacteriophora-EO7: 0.0±0.0). There was no significant difference in the efficacy of the isolates at all concentrations and for all time periods. The effectiveness of both isolates against this pest should be tested at different temperatures and humidities and on different surfaces such as ceramic, concrete and parquet.

Keywords:

Blatta lateralis, entomopathogenic nematode, biological control,

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