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Ortam neminin, Beauveria bassiana (Balsamo) Vuillemin ve diatomlu toprağın Ekin kambur biti Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae)’ya karşı kombine uygulaması üzerine etkinliğinin araştırılması

Entomopatojen fungus (EF) ve diyatom toprağının (DE) depolanmış tahıl zararlılarına karşı tek başlarına veya birlikte etkili olduğu bilinmektedir. Bu çalışmada, bir Türk DE (ACN) ile birleştirilmiş iki yerel Beauveria bassiana (Balsamo) Vuillemin izolatı (5-4, 1-1) seçilen ortam koşullarının birleşik uygulamaların etkinliği üzerindeki etkisini ortaya çıkarmak için üç nispi nemde (%45, %55, %65) ve iki sıcaklık değerinde (25ºC, 30ºC) Ekin kambur biti Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae) erginlerine karşı test edilmiştir. Denemeler için 40 g buğday alan 50 ml kapasiteli santrifüj tüpleri kullanılmıştır. Kontrol için sadece buğday kullanılmıştır. EF ve DE ayrı ayrı 300 ppm (w/w) konsantrasyonunda ve birlikte 300ppm+300ppm konsantrasyonunda kullanılmıştır. Böcek ölümleri 7-14 gün sonra kaydedilmiştir. Elde edilen sonuçlara göre, B. bassiana'nın DE ile birlikte uygulanması, test edilen nispi nem seviyelerinde R. dominica'ya karşı aynı derecede etkilidir ve bu yüzden etkinlik kaybı olmadan bir dizi ortam koşulunda kullanılabilir.

Anahtar Kelimeler:

Beauveria basiana, Diatom toprağı, Rhyzopertha dominica, Nispi nem

Investigation of the effects of ambient humidity on the combined application of Beauveria bassiana (Balsamo) Vuillemin and diatomaceous earth against the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae)

Entomopathogenic fungi (EF) and diatomaceous earth (DE) are effective alone or in combination against stored-grain insects. In this study, two Turkish isolates of Beauveria bassiana (Balsamo) Vuillemin and a Turkish DE were tested separately and in combination at three relative humidities (45%, 55%, 65%) and two temperatures (25ºC, 30ºC) to determine their efficacy against adults of the lesser grain borer Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae). For the bioassays, 50 mL centrifuge tubes containing 40 g of wheat were used. For the controls, wheat alone was used. The EFs and DE were used at 300 ppm (w/w) individually and 300 ppm+300 ppm in combination. Insect mortality was recorded after 7 and 14 days. The application of B. bassiana with DE was equally effective against R. dominica at the three levels of RH used. Therefore, the further testing of combinations of entomopathogenic fungi and diatomaceous earths in grain storages under a range of ambient condition is recommended.

Keywords:

: Beauveria basiana, Diatomaceous earth, Rhyzopertha dominica, Relative humidity,

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