Van Gölüne Endemik Olan İnci Kefali (Chalcalburnus tarichi PALLAS 1811) Kromozomlar›n›n C, G ve Restriksiyon Endonükleazlar (Alu I, Nhe I, Hae III, Mbo I, Hinf I) ile Bantlanması
Sazangiller familyas›na ait Chalcalburnus tarichi’nin kromozomlar›n›n say› ve yap›lar› incelenerek karyotipleri yap›lm›flt›r. Bu çal›flmada kullan›lan bal›klar Van Gölü’nden a¤larla yakalanarak laboratuvara getirilmifltir. Bal›klar›n kar›n bofllu¤una gramlar›na 0,01 ml gelecek flekilde % 0,6’l›k kolsiflin enjekte edilmifltir. Metafaz incelemeleri sonucunda C. tarichi’nin 2n = 50 kromozoma sahip oldu¤u belirlenmifltir. Karyotiplerinin ise 8 metasentrik, 5 submetasentrik ve 12 akrosentrik kromozom çiftlerinden olufltu¤u saptanm›flt›r. Bu türde cinsiyetle ba¤lant›l› herhangi bir kromozom tespit edilememifltir. C. tarichi kromozomlar› befl restriksiyon endonükleazla muamele edilmifl, Giemsa ile boyanm›fl ve bant örnekleri incelenmifltir. Alu I enzimi baryum hidroksit etkileflimi ile ortaya ç›kan C-banda benzer bant örnekleri üretmifltir. Hae III, Hinf I, Nhe I ve Mbo I enzimleri ise G-banda benzer bant örnekleri üretmifltir. Restriksiyon endonükleazlar Giemsa ile boyanma oran›n› belirgin bir flekilde düflürmüfltür.
C, G and Restriction Endonuclease (Alu I, Nhe I, Hae III, Mbo I, Hinf I) Banding of the Chromosomes in Chalcalburnus tarichi (PALLAS 1811) Endemic to Lake Van
Karyotype analysis was performed in Chalcalburnus tarichi specimens by investigating the number and structures of their chromosomes. The fish used in this study were caught with fishing nets from Lake Van and taken to the laboratory. The fish were injected i.p. with 0.01 ml/g body weight doses of a 0.6% solution of a colchicine for 190 min. As a result of the metaphase investigation we determined that C. tarichi had 2n = 50 chromosomes. Their karyotypes were determined to be composed of 8 metacentric, 5 submetacentric and 12 acrocentric chromosome pairs. We were unable to identify any sex-related chromosomes in this species. C. tarichi chromosomes were treated with 5 restriction endonucleases stained with Giemsa and examined for banding patterns. The enzymes Alu I revealed banding patterns similar to the C-bands produced by treatment with barium hydroxide. The enzymes Hae III, Hinf I, Nhe I and Mbo I revealed banding patterns similar to those of G-bands. The restriction endonucleases markedly reduced the extent of Giemsa staining.
