Mutlu KÜÇÜK, Mehmet KAYA, Nurcan ORHAN, Oğuzhan EKİZOĞLU, Erol ARSLAN, Nadir ARICAN, R.candan GÜRSES, İmdat ELMAS, Rivaze KALAYCI, Bülent AHISHALI

120130

The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia

The pathophysiological mechanisms underlying blood-brain barrier (BBB) disruption in preeclampsia/eclampsia have not been elucidated. This study investigated the effect of pentylenetetrazole (PTZ)-induced seizures on the functional and structural properties of the BBB during N (omega)-nitro-L-arginine methyl ester (L-NAME)-induced hypertension and proteinuria in pregnant rats. Animals were treated with L-NAME for 10 days, beginning on day 10 of pregnancy. The BBB permeability was determined by measurements of sodium fluorescein (NaFlu) extravasation into the brain. Occludin and aquaporin (AQP)-4 immunoreactivities were evaluated in brain sections. Severe proteinuria and significantly increased arterial blood pressure were observed following L-NAME treatment. PTZ-induced seizures in pregnant rats treated with L-NAME increased NaFlu levels in all of the brain regions analyzed (P < 0.01). A significant increase in the extravasation of NaFlu was also observed in the diencephalon of intact pregnant rats treated with PTZ. PTZ-induced seizures in both L-NAME-treated and untreated pregnant rats significantly decreased occludin immunoreactivity in the hippocampal capillaries. L-NAME administration significantly increased AQP-4 immunoreactivity in the astrocytic endfeet surrounding the parietal cortex microvessels of PTZ-treated and untreated pregnant rats. These findings suggest that PTZ-induced seizures lead to a severe disruption of the BBB in preeclampsia and that the paracellular pathway may play an important role in increased BBB permeability in this context.
Anahtar Kelimeler:

Key words: Pregnancy, hypertension, sodium fluorescein, aquaporin-4, occluding

The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia

The pathophysiological mechanisms underlying blood-brain barrier (BBB) disruption in preeclampsia/eclampsia have not been elucidated. This study investigated the effect of pentylenetetrazole (PTZ)-induced seizures on the functional and structural properties of the BBB during N (omega)-nitro-L-arginine methyl ester (L-NAME)-induced hypertension and proteinuria in pregnant rats. Animals were treated with L-NAME for 10 days, beginning on day 10 of pregnancy. The BBB permeability was determined by measurements of sodium fluorescein (NaFlu) extravasation into the brain. Occludin and aquaporin (AQP)-4 immunoreactivities were evaluated in brain sections. Severe proteinuria and significantly increased arterial blood pressure were observed following L-NAME treatment. PTZ-induced seizures in pregnant rats treated with L-NAME increased NaFlu levels in all of the brain regions analyzed (P < 0.01). A significant increase in the extravasation of NaFlu was also observed in the diencephalon of intact pregnant rats treated with PTZ. PTZ-induced seizures in both L-NAME-treated and untreated pregnant rats significantly decreased occludin immunoreactivity in the hippocampal capillaries. L-NAME administration significantly increased AQP-4 immunoreactivity in the astrocytic endfeet surrounding the parietal cortex microvessels of PTZ-treated and untreated pregnant rats. These findings suggest that PTZ-induced seizures lead to a severe disruption of the BBB in preeclampsia and that the paracellular pathway may play an important role in increased BBB permeability in this context.
Keywords:

Key words: Pregnancy, hypertension, sodium fluorescein, aquaporin-4, occluding,

PDF
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: 6
  • Yayıncı: TÜBİTAK
Arşiv
Sayıdaki Diğer Makaleler

Assessing the nutritive value of fruit and vegetable residues as ruminant feed

Keyvan KARKOODI, Hassan FAZAELI, S. Sadegh MIRGHAFFARI

The effect of maternal undernutrition on muscle development in the ovine fetus

Mukaddes ÖZCAN, Berjan DEMİRTAŞ

The effect of maternal undernutrition on muscle development in the ovine fetus*

Berjan DEMİRTAŞ, Mukaddes ÖZCAN

Immune system dysfunction in broiler chickens experimentally inoculated with fowl adenovirus serotype-4 associated with inclusion body hepatitis hydropericardium syndrome

İftikhar HUSSAIN, Muhammad Shahid MAHMOOD, Muhammad İmran ARSHAD, Masood AKHTAR, Fazal MAHMOOD, Azhar RAFIQUE

Effect of a simulate show jumping competition on the blood gas profile of horses trained for show jumping

Francesco FAZIO*, Vanessa MESSINA, Stefania CASELLA, Claudia GIANNETTO, Simona MARAFIOTI, Giuseppe PICCIONE

The influence of egg shell crack types on hatchability and chick quality

Mozhdeh Moosanezhad KHABISI, Ahmad SALAHI, Seyed Naser MOUSAVI

The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia

Mutlu KÜÇÜK, Mehmet KAYA, Nurcan ORHAN, Oğuzhan EKİZOĞLU, Erol ARSLAN, Nadir ARICAN, R.candan GÜRSES, İmdat ELMAS, Rivaze KALAYCI, Bülent AHISHALI

Probiotic properties, sensory qualities, and storage stability of probiotic banana yogurts

Songül ÇAKMAKÇI, Bülent ÇETİN, Tamer TURGUT, Mustafa GÜRSES, Ahmet ERDOĞAN

A study on paranal sinus micromorphometrical parameters in dogs of different ages

İvaylo STEFANOV*

Probiyotikli muzlu yoğurtların probiyotik özellikleri, duyusal kalitesi ve depolama stabilitesi

Songül ÇAKMAKÇI, Bülent ÇETİN, Tamer TURGUT, Mustafa GÜRSES, Ahmet ERDOĞAN