The effect of maternal undernutrition on muscle development in the ovine fetus

Koyunda gebeliğin ilk yarısında maternal besin kısıtlamasının gelişmiş fötusta kas gelişimi üzerine etkisi iki farklı deneyle yürütüldü. Her iki deneyde de koyunlar rastgele kontrol ve besin kısıtlaması yapılan gruplara ayrıldı. İlk deneyde hafif maternal besin kısıtlaması günlük besin ihtiyacı (GBİ)’nın % 85’i verilerek gebeliğin 0 ve 70. günleri arasında uygulandı. İkinci deneyde şiddetli maternal besin kısıtlaması (% 50 GBİ) gebeliğin 0 ve 70. günleri arasında uygulandı. Her iki deneyde kontrol grupları tüm gebelik süresince % 100 GBİ ile beslendi. Koyunlar her iki deneyde de gebeliğin 126 ± 1. gününde sakrifi ye edildi. Fötuslar çıkarılarak semitendinossus kasları diseke edildi. Kesitler alınarak alkali-ATPaz ile boyandı. Kastaki primer, sekonder ve toplam kas lifi sayıları hesaplandı. Her iki deneyde de primer, sekonder ve toplam kas lifleri sayılarında belirgin bir azalma saptandı (P < 0,05). Primer liflerin sayılarındaki azalma koyunda merkezi, büyük ve alkali-ATPaz negatif (yavaş) liflerin bir kısmının morfolojik olarak primer lifler olarak gözükmesine rağmen aslında sekonder lifler olmalarından kaynaklanabilir. Bu durumda gerçek primer liflerin etkilenmediği söylenebilir.

Koyunda maternal besin kısıtlamasının fötus kas gelişimine etkisi

The effect of maternal undernutrition on muscle development of near-term ovine fetuses was studied in 2 experiments. In each experiment, Welsh mountain ewes were randomly assigned to either control (C) or nutrientrestricted (R) groups. In the first experiment, mild maternal undernutrition (85% of daily nutritional requirement (DNR)), and in the second experiment, severe maternal undernutrition (50% of DNR), were imposed between 0 and 70 days of gestation (dg). Controls for both groups were maintained on 100% of their DNR throughout the experiment. In each experiment, ewes were killed at 126 ± 1 dg by an intravenous injection of pentobarbitone. The semitendinosus muscle was dissected, and sections were stained for alkali-stable ATPase. The total number of primary and secondary fibers and the total number of fibers were estimated. In both experiments, there was a significant decrease in primary, secondary, and total fiber numbers (P < 0.05 for each). We speculate that the decrease in primary fiber number in both experiments is due to some of the large, central, and alkali-ATPase-negative (slow) fibers at near-term being secondary fibers. Therefore, the real primary fibers may not be affected.

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