The effect of oleic and linoleic acids on in vitro bovine embryonic development and embryo quality

The present study was aimed at the investigation of the effects of oleic and linoleic acid on the in vitro development of Anatolian native black crossbred bovine embryos. Following the in vitro maturation and fertilization of oocytes, embryonic development stages were monitored using embryo culture medium supplemented with three different doses of oleic and linoleic acid (10, 100, and 1000 µM). Examination of the culture media 48 h after fertilization demonstrated that linoleic acid had no effect on embryo cleavage rates, while oleic acid produced a significant increase in cleavage rates (P < 0.01). It was determined that the highest dose of linoleic acid (1000 µM) and all three doses of oleic acid significantly increased the proportion of cultured oocytes developing to the morula-blastocyst stage (P < 0.001). As a result, while oleic acid significantly increased (P < 0.001) the proportion of embryos developing to the morula-blastocyst stage, linoleic acid had limited effects on embryonic development and quality. Thus, it was concluded that the supplementation of Anatolian native black crossbred bovine embryo cultures with oleic acid may induce positive effects on embryonic development and quality.

Anahtar Kelimeler:

Oleic acid, linoleic acid, bovine embryo, blastocyst

The effect of oleic and linoleic acids on in vitro bovine embryonic development and embryo quality

Keywords:

Oleic acid, linoleic acid, bovine embryo, blastocyst,

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Lonergan P, Rizos D, Nord F, Boland MP. Factors influencing oocyte and embryo quality in cattle. Reprod Nutr Dev 2002; 41: 427–437.
Livingston L, Rich K, Mackenzie S, Godkin JD. Glutathione content and antioxidant enzyme expression of in vivo matured sheep oocytes. Anim Reprod Sci 2009; 116: 265–273.
Guerin P, Mouatassim SE, Menezo Y. Oxidative stress and protection against reactive oxygen species in the pre- implantation embryo and its surroundings. Hum Reprod Update 2001; 7: 175–189.
Johnson MH, Nasr-Esfahani MH. Radical solutions and cultural problems: could free oxygen radicals be responsible for the impaired development of preimplantation mammalian embryos in vitro? BioEssays 1994; 16: 31–38.
Bucak MN, Satılmış M, Karaşahin T, Kızıl SH, Akyol N. Effect of antioxidants added to culture media on the in vitro development of bovine embryos. Kafkas Univ Vet Fak Derg 2010; 16: 69–74. 6. Gordon I. Laboratory Production of Cattle Embryos. London, UK: Cambridge University Press; 1994.
Gasparrini B, Boccia L, Marchandise J, Di Palo R, George F, Donnay I, Zicarelli L. Enrichment of in vitro maturation medium for buffalo (Bubalus bubalis) oocytes with thiol compounds: effects of cysteine on glutathione synthesis and embryo development. Theriogenology 2006; 65: 275–287.
Lott WM, Anchamparuthy VM, Mcgilliard ML, Mullarky IK, Gwazdauskas FC. Influence of cysteine in conjunction with growth factors on the development of in vitro-produced bovine embryos. Reprod Domest Anim 2010; 46: 585–594.
Camargo LSA, Viana JHM, Sá WF, Ferreira AM, Ramos AA, Vale Filho VR. Factors influencing in vitro embryo production. Anim Reprod Sci 2006; 3: 19–28.
Khandoker MAM, Tsujii H. Effect of exogenous fatty acids on in vitro development of rat embryos. Asian Australas J Anim Sci 1999; 12: 169–173.
Kim JY, Kinoshita M, Ohnishi M, Fukui Y. Lipid and fatty acid analysis of fresh and frozen-thawed immature and in vitro matured bovine oocytes. Reproduction 2001; 122: 31–38.
Marei WF, Wathes DC, Fouladi-Nashta AA. The effect of linolenic acid on bovine oocyte maturation and development. Biol Reprod 2009; 81: 1064–1072.
Fagali N, Catala A. Antioxidant activity of conjugated linoleic acid isomers, linoleic acid and its methyl ester determined by photoemission and DPPH techniques. Biophys Chem 2008; 137: 56–62.
Wang R, Kern JT, Goodfriend TL, Ball DL, Luesch H. Activation of the antioxidant response element by specific oxidized metabolites of linoleic acid. Prostaglandins Leukot Essent Fatty Acids 2009; 81: 53–59.
Pereira RM, Baptista MC, Vasques MI, Horta AEM, Portugal PV, Bessa RJB, Chagas E, Silva J, Silva Pereira M, Marques CC. Cryo-survival of bovine blastocysts is enhanced by culture with trans-10 cis-12 conjugated linoleic acid (10t, 12c CLA). Anim Reprod Sci 2007; 98: 293–301.
Enright BP, Lonergan P, Dinnyes A, Fair T, Ward FA, Yang X, Boland MP. Culture of in vitro produced bovine zygotes in vitro vs in vivo: implications for early embryo development and quality. Theriogenology 2000; 54: 659–673.
Feugang JM, Camargo-Rodriguez O, Memili E. Culture systems for bovine embryos. Livest Sci 2009; 121: 141–149.
Takenaka M, Hoirichi T, Yanagimachi R. Effects of light on development of mammalian zygotes. P Natl Acad Sci USA 2007; 104: 14289–14293.
Feugang JM, De Roover R, Moens A, Leonard S, Dessy F, Donnay I. Addition of beta-mercaptoethanol or trolox at the morula/blastocyst stage improves the quality of bovine blastocyst and prevents induction of apoptosis and degeneration by prooxidant agents. Theriogenology 2004; 61: 71–90.
Darwich AA, Perreau C, Petit MH, Papillier P, Dupont J, Guillaume D, Mermillod P, Guignot F. Effects of PUFA on embryo cryoresistance, gene expression and AMPKα phosphorylation in IVF-derived bovine embryos. Prostag Other Lipid Mediat 2010; 93: 30–36.
Hosseini SM, Forouzanfer M, Hajian M, Asgari V, Abedi P, Hosseini L, Ostadhosseini S, Moulavi F, Asfahani-Langrroodi M, Sadeghi H et al. Antioxidant supplementation of culture medium during embryo development and/or after vitrification- warming; which is the most important? J Assist Reprod Genet 2009; 26: 355–364.
Öztürkler Y, Yıldız S, Güngör Ö, Pancarcı ŞM, Kaçar C, Arı UÇ. The effects of L-ergothioneine and L-ascorbic acid on the in vitro maturation (IVM) and development (IVC) of sheep oocytes. Kafkas Univ Vet Fak Derg 2010; 16: 757–763.
Lapa M, Marques CC, Alves SP, Vasques MI, Baptista MC, Carvalhais I, Silva PM, Horta AEM, Bessa RJP, Pereira RM. Effect of trans-10 cis-12 conjugated linoleic acid on bovine oocyte competence and fatty acid composition. Reprod Domest Anim 2011; 46: 904–910.
Marei WF, Wathes DC, Fouladi-Nashta AA. Impact of linoleic acid on bovine oocyte maturation and embryo development. Reproduction 2010; 139: 979–988.