Ying LIU, Xue Mei YIN, Ri Wei XIA, Yong Jiu HUO, Guo Qiang ZHU, Sheng Long WU, Wen Bin BAO

Association between the MUC4 g.243A > G polymorphism and immune and production traits in Large White pigs

PCR-RFLP analysis of intron 17 of the MUC4 gene was conducted in Large White pigs and associations with cytokine levels (IFN-?, IL-1Ã, IL-4, IL-6, IL-8, IL-10, TGF-Ã, and TNF-?), production traits (100-kg-weight day age, back-fat depth, and eye muscle depth), and reproductive performance within different litters (total number born, number born alive, number in live weaning litter) were analyzed with the aim of identifying genetic markers of disease resistance. The results revealed AA, AG, and GG genotypes, with an A243G mutation identified in the GG genotype. Correlation analysis indicated that IL-8 and IL-10 levels and 100-kg-weight day age were significantly higher in the GG genotype compared with AA and AG genotypes (P < 0.05). No significant differences were detected in IL-8 and IL-10 levels or 100-kg-weight day age for AA and AG genotypes. The levels of other cytokine levels and reproductive performance from the first to fourth litter did not significantly differ among the 3 genotypes (P > 0.05). These data indicate that MUC4 g.243A>G may enhance the antidisease capability and affect the growth rate of groups in the fattening period of Large White pigs without detrimental influences on resistance to disease, other production traits, and reproductive performance.

Anahtar Kelimeler:

Swine, MUC4 gene, genetic marker, immunity index, economic traits

Association between the MUC4 g.243A > G polymorphism and immune and production traits in Large White pigs

Keywords:

Swine, MUC4 gene, genetic marker, immunity index, economic traits,

PDF

___

Jin LZ, Zhao X. Intestinal receptors for adhesive fimbriae of enterotoxigenic Escherichia coli (ETEC) K88 in swine - a review. Appl Microbiol Biot 2000; 54: 311–318.
Van Den Broeck W, Cox E, Oudega B, Goddeeris BM. The F4 fimbrial antigen of Escherichia coli and its receptors. Vet Microbiol 2000; 71: 223–244.
Gibbons RA, Sellwood R, Burrows M, Hunter PA. Inheritance of resistance to neonatal E. coli diarrhoea in the pig: examination of the genetic system. Theor Appl Genet 1977; 51: 65–70.
Sellwood R. Escherichia coli diarrhoea in pigs with or without the K88 receptor. Vet Rec 1979; 105: 228–230.
Marquardt RR, Jin LZ, Kim JW, Fang L, Frohlich AA, Baidoo SK. Passive protective effect of egg-yolk antibodies against enterotoxigenic Escherichia coli K88+ infection in neonatal and early-weaned piglets. FEMS Immunol Med Mic 1999; 23: 283–288.
Corfield AP, Carroll D, Myerscough N, Probert CS. Mucins in the gastrointestinal tract in health and disease. Front Biosci 2001; 6: D1321–1357.
Moniaux N, Escande F, Porchet N, Aubert JP, Batra SK. Structural organization and classification of the human mucin genes. Front Biosci 2001; 6: D1192–1206.
Python P, Jörg H, Neuenschwander S, Hagger C, Stricker C, Bürgi E, Bertschinger HU, Stranzinger G, Vögeli P. Fine- mapping of the intestinal receptor locus for enterotoxigenic Escherichia coli F4ac on porcine chromosome 13. Anim Genet 2002; 33: 441–447.
Jİrgensen CB, Cirera S, Anderson SI, Archibald AL, Raudsepp T, Chowdhary B, Edfors-Lilja I, Andersson L, Fredholm M. Linkage and comparative mapping of the locus controlling susceptibility towards E. coli F4ab/ac diarrhoea in pigs. Cytogenet Genome Res 2003; 102: 157–162.
Joller D, Jİrgensen CB, Bertschinger HU, Python P, Edfors I, Cirera S, Archibald AL, Bürgi E, Karlskov-Mortensen P, Andersson L et al. Refined localization of the Escherichia coli F4ab/F4ac receptor locus on pig chromosome 13. Anim Genet 2009; 40: 749–752.
Erickson AK, Baker DR, Bosworth BT, Casey TA, Benfield DA, Francis DH. Characterization of porcine intestinal receptors for the K88ac fimbrial adhesin of Escherichia coli as mucin- type sialoglycoproteins. Infect Immune 1994; 62: 5404–5410.
Jacobsen M, Kracht SS, Esteso G, Cirera S, Edfors I, Archibald AL, Bendixen C, Andersson L, Fredholm M, Jİrgensen CB. Refined candidate region specified by haplotype sharing for Escherichia coli F4ab/F4ac susceptibility alleles in pigs. Anim Genet 2010; 41: 21–25.
Jacobsen M, Cirera S, Joller D, Esteso G, Kracht SS, Edfors I, Bendixen C, Archibald AL, Vogeli P, Neuenschwander S et al. Characterisation of five candidate genes within the ETEC F4ab/ac candidate region in pigs. BMC Research Notes 2011; 4: 225.
Joller D, Jİrgensen CB, Bertschinger HU, Burgi E, Stannarius C, Mortensen PK, Cirera S, Archibald A, Genini S, Edfors-Lilja I et al. Refined linkage mapping of the Escherichia coli F4ac receptor gene on pig chromosome 13. In: Proceedings of the 30th International Conference on Animal Genetics, Brazil, 2006; poster ID B512.
Peng QL, Ren J, Yan XM, Huang X, Tang H, Wang YZ, Zhang B, Huang LS. The g.243A > G mutation in intron 17 of MUC4 is significantly associated with susceptibility/resistance to ETEC F4ab/ac infection in pigs. Anim Genet 2007; 38: 397–400.
Balcells I, Castelló A, Mercadé A, Noguera JL, Fernández- Rodríguez A, Sànchez A, Tomàs A. Analysis of porcine MUC4 gene as a candidate gene for prolificacy QTL on SSC13 in an Iberian × Meishan F2 population. BMC Genet 2011; 12: 93.
Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory Press; 1989.
Fontanesi L, Bertolini F, Dall’Olio S, Buttazzoni L, Gallo M, Russo V. Analysis of association between the MUC4 g.8227C > G Polymorphism and production traits in Italian heavy pigs using a selective genotyping approach. Anim Biotechnol 2012; 23: 147–155.