The effect of diethylnitrosamine on the levels of sialic acid lipid-bound sialic acid and enzyme activities of transferase in rat serum

Bu çalışmanın amacı kimyasal bir karsinojen olan Dietilnitrosamin (DENA) 'in serum siyalik asit (SA) ve lipid-bağlı siyalik asit (LBSA) ile alanin transaminaz (ALT), aspartat transaminaz (AST) ve gama-glutamiltransferaz (GGT) enzim düzeylerine etkisini araştırmaktır. Çalışmada 180 dişi sıçan kullanılmıştır. Sıçanlar kontrol ve deneme grupları olarak üçe ayrılmıştır. Her grup 60'ar hayvandan oluşmuştur. Kontrol grubu olarak kullanılan ratlara periton içi (i.p) serum fizyolojik verilmiştir. Deneme-I grubuna serum fizyolojik içinde çözülmüş 100 mg/kg-vücut ağırlığına DENA, Deneme-II grubundaki sıçanlara ise 200 mg/kg-vücut ağırlığına DENA i.p. olarak verilmiştir. Tüm gruplara DENA tek doz olarak verilmiştir. Kan örnekleri dört ay boyunca her ay her bir gruptan rastgele seçilen sıçanlardan sağlanmıştır. Serumda SA, LBSA düzeyleri ve ALT, AST, GGT enzim aktiviteleri ölçülmüştür. Deneme-1 ve Deneme-11 gruplarının çalışma süresince serum SA, LBSA, ALT, AST, GGT ortalama değerleri kontrol grubunun değerleriyle karşılaştırıldığında, farklılıklar istatistik! olarak anlamlı bulunmuştur. Çalışma sonuçları değerlendirildiğinde, enzim aktiviteleri ile SA, ve LBSA düzeylerindeki artışlar, karaciğer hücre dejenerasyonundan kaynaklanabileceğini düşündürmektedir. Sonuçlar, tümör markırlarının (SA, LBSA) ve karaciğer enzim aktivitelerinin beraber değerlendirilmesinin tumoral değişimlerin teşhisinde faydalı olabileceğini önermektedir.

Dietilnitrosamin'in sıçanlarda serum siyalik asit, lipid bağlı siyalik asit ve transferaz enzim aktivitelerine etkisi

The aim of this study was to investigate the effects of a chemical carcinogen, Diethylnitrosamine (DENA), on these markers, sialic acid (SA), Iipid-bound sialic acid (LBSA) and the liver enzymes aspartate aminotransferase, alanine aminotransferase, and gamma-glutamyltransferase. In this research 180 male Wistar albino rats were used. Rats were divided equally into three groups. Intraperitoneal (i.p.) injection of saline was given to rats in the control group. Treatment group I was given i.p. 100 mg/kg-body weight DENA dissolved in saline. Treatment group II was given i.p. 200 mg/kg-body-weight DENA dissolved in saline. These doses were given to each groupjust once. Blood samples were taken from fifteen rats, which were chosen randomly from each group per month during the four months. Levels of SA and LBSA and activities of ALT, AST and GGT were determined in serum. Significant elevations in the serum levels of SA and LBSA and activities of ALT, AST and GGT were observed in treatment groups when compared with the controls. In this study, the high levels of enzyme activities, sialic acid, and Iipid-bound sialic acid led to hepatocellular degeneration. The results suggest that combined evaluation of these markers (SA and LBSA) and liver enzyme activities can be useful in predicting malignant change.

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