The Effects of Melatonin Hormone on Carbonic Anhydrase Enzyme Activity in Rainbow Trout (Oncorhynchus mykiss) Erythrocytes In Vitro and In Vivo

The effects of melatonin hormone on rainbow trout (Oncorhynchus mykiss) erythrocyte carbonic anhydrase enzyme purified by using Sepharose 4B-L tyrosine-sulfanylamide affinity gel chromatography were investigated in vitro and in vivo. Carbonic anhydrase from Oncorhynchus mykiss erythrocytes was purified with a specific activity of ~400 EU/mg protein and ~200-fold in a yield of ~20. Melatonin did not inhibit carbonic anhydrase enzyme in vitro. Carbonic anhydrase activity of the control group was determined as 1597.7 ± 429.0 EU/g hemoglobin in vivo. Carbonic anhydrase activities were measured at 1, 3 and 5 h by following melatonin injection. The corresponding mean activities of melatonin were 1406.4 ± 372.1, 1346.3 ± 477.4 and 783.8 ± 494.3 EU/g hemoglobin respectively. Oncorhynchus mykiss erythrocyte carbonic anhydrase was inhibited significantly by melatonin hormone 5 h after injection in vivo (P < 0.05). Data from the present study suggest that excessive usage of this hormone should be avoided in fish reproduction.

Anahtar Kelimeler:

Carbonic anhydrase, erythrocyte, melatonin, Oncorhynchus mykiss, in vitro, in vivo

The Effects of Melatonin Hormone on Carbonic Anhydrase Enzyme Activity in Rainbow Trout (Oncorhynchus mykiss) Erythrocytes In Vitro and In Vivo

Keywords:

Carbonic anhydrase, erythrocyte, melatonin, Oncorhynchus mykiss, in vitro, in vivo,

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