Halil BIYIK, Hümeyra ÜNSAL, Cengiz ÜNSAL, Muharrem BALKAYA

The effects of qualitative and quantitative protein malnutrition on cecal microbiota in wistar rats with or vithout neutrophil suppression

Gastrointestinal mikfobiyotanin diyetten etkilendiği bilinmektedir. Bu çalışmada nötrofii baskılanmış veya baskılanmamış erkek Wistar sıçanlarında kalitatif ve kantitatif protein malnutrisyonunun sekum mikrobiotasına etkileri araştırıldı. Toplam 43 hayvan rasgele 7 gruba ayrıldı. Grup I (kontrol) normal yem, grup II, III ve IV protein içermeyen diyet, grup V, VI ve Vll % 20jelatin içeren diyetle 35 gün süreyle ad libitum beslendi. Ayrıca, grup I, II ve Ve fizyolojik tuzlu su, grup 111 ve Vl'ya normal tavşan serumu ve grup IV ve VU'ye anti-rat nötrofii antikoru içeren tavşan serumu haftada bir kez i.p. yolla verildi. Deney sonunda hayvanlar ötenazi edildi ve aseptik şartlarda sekumlar çıkartıldı. Sekum içeriklerinde total aerob ve anaerob bakteri, lactobacilli ve Enterobacteriaceae sayıları belirlendi. Çalışma sonuçları diyeter proteinlerin miktarının ve kalitesinin sekal aerob ve lactobacilli konsantrasyonlarını etkilediğini, buna karşın total anaerob ve Enterobactericeae konsantrasyonlarını etkilemediğini gösterdi. Protein malnutrisyonu sekal lactobacil sayısında azalmaya neden oldu. Kontrol grubu ile fizyolojik tuzlu su uygulanan malnutrisyon grupları arasındaki fark önemliydi (P < 0,0001). Ayrıca malnutrisyonlu gruplar arasında yapılan karşılaştırmada grup lll'ün ortalama lactobacilli sayısının grup 11, IV, V, VI ve Vll'dekinden daha yüksek olduğu görüldü (sırasıyla P < 0,05, P < 0,01, P < 0,05, P < 0,01 ve P < 0,05). Çalışmanın sonuçları, diyetteki proteinin sekal mikrobiyotayı etkilediğini ve bu etkinin hayvanın bağışıklık sisteminin fonksiyonel durumu tarafından değiştirilebildiğini göstermektedir.

Nötrofil baskılanmış ve baskılanmamış wistar sıçanlarında kalitatif ve kantitatif protein yetersizliklerinin sekal mikroorganizmalara etkileri

Gastrointestinal microbiota has been claimed to be affected by the diet consumed. In this study the effects of severe qualitative and quantitative protein malnutrition on cecal microbiota of male Wistar rats with or without neutrophil depletion were investigated. A total of 43 animals were divided randomly into 7 groups. Group I (control) was given a complete chow diet, groups II, 111 and IV received almost protein free (N-free) diet and groups V, VI and VII received a 20% gelatin containing diet for 35 days ad libitum. In addition, groups I, II and V were given physiologic saline, groups 111 and VI were given normal rabbit serum while groups IV and VII were given anti-rat neutrophil antibody containing rabbit serum (antineutrophil serum) by intraperitoneal route weekly. At the end of experiment, the animals were euthanized; the ceca were removed aseptically and total aerobe and anaerobe microorganisms, lactobacilli and Enterobacteriaceae were isolated from cecal contents. The evidences indicated that the growth of cecal aerobe microorganisms and lactobacilli was influenced by the quantity and quality of the dietary protein, while total anaerobes and Enterobacteriaceae remained unaffected. The count of cecal lactobacilli was decreased by protein malnutrition. The differences between the control group and both malnourished groups given SP were confirmed statistically (P < 0.0001). In addition, the comparisons among different malnourished groups showed that the mean count of lactobacilli in group 111 was significantly higher than that of groups II, IV, V, VI and VII (P < 0.05, P < 0.01, P < 0.05, P < 0.01 and P < 0.05, respectively). Also, for total aerobe microorganisms the differences between group IV and groups VI and Vll and between group II and group VI were confirmed (P < 0.01, P < 0.05 and P < 0.05, respectively). We conclude that dietary protein could influence the cecal microbiota, and this effect could be modified by actual immune status of animals.

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