Ebrahim RAHIMI, Hassan MOMMTAZ, Amir SHAKERIAN, Hamid Reza KAVYANI

The detection of classical enterotoxins of Staphylococcus aureus in raw cow milk using the ELISA method

Milk and dairy products are frequently contaminated with exterotoxigenic Staphylococcus aureus, which is often involved in Staphylococcal food poisoning. The aim of the present study is to investigate the presence of classical enterotoxins of S. aureus in raw milk that was produced in Isfahan, Iran. For this purpose, 72 milk samples were collected from the bulk milk tanks of 12 milking farms during 3 different seasons (winter, spring, and summer) and tested for the presence of staphylococcal enterotoxins (SEs) using the enzyme-linked immunosorbent assay (ELISA) method. Out of 72 samples studied, 15 samples (20.8%) were positive for at least 1 SE. Of these, 12 (16.7%) were positive for SEA, 9 (12.5%) for SED, and 6 (8.3%) for SEC. None of the samples was positive for SEB or SEE. Statistical evaluation showed that there were not any significant differences (P > 0.05) between the presence of SEs in the milk samples tested in winter, spring, and summer. Further studies should be carried out to investigate the presence of these toxins in different foods and their roles in food poisoning.

Anahtar Kelimeler:

Key words: Enterotoxins, milk, Staphylococcus aureus

The detection of classical enterotoxins of Staphylococcus aureus in raw cow milk using the ELISA method

Keywords:

Key words: Enterotoxins, milk, Staphylococcus aureus,

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Le Loir, Y., Baron, F., Gautier, M.: Staphylococcus aureus and food poisoning. Genet. Mol. Res., 2003; 2: 63-76.
De Buyser, M.L., Dufour, B., Maire, M., Lafarge, V.: Implication of milk and milk products in food-borne diseases in France and in diff erent industrialized countries. Int. J. Food Microbiol., 2001; 67: 1-17.
Jorgensen, H.J., Mork, T., Hogasen, H.R., Rovik, L.M.: Enterotoxigenic Staphylococcus aureus in bulk milk in Norway. J. Appl. Microbiol., 2005; 99: 158-167.
Brasca, M., Morandi, S., Vanoni, L., Colombo, L., Lodi, R.: Th e infl uence of diff erent cultural conditions on the development and toxinogenesis of Staphylococcus aureus. Sci. Tec. Latt. Cas., 2005; 56: 105-115.
Asao, T., Kumeda, Y., Kawai, T., Shibata, T., Oda, H., Haruki, K., Nakazawa, H., Kozyki, S.: An extensive outbreak of staphylococcal food poisoning due to low-fat milk in Japan: estimation of enterotoxin A in the incriminated milk and powdered skim milk. Epidemiol. Infect., 2003; 130: 33-40.
da Silva, E.R., do Carmo, L.S., da Silva, N.: Detection of the enterotoxin A, B and C genes in Staphylococcus aureus from goat and bovine mastitis in Brazilian dairy herds. Vet. Microbiol., 2005; 106: 103-107.
Balaban, N., Rasooly, A.: Staphylococcal enterotoxins. Int. J. Food Microbiol., 2000; 61: 1-10.
Morandi., S., Brasca, M., Lodi, R., Cremonesi, P., Castiglioni, B.: Detection of classical enterotoxins and identifi cation of enterotoxin genes in Staphylococcus aureus from milk and dairy products. Vet. Microbiol., 2007; 124: 66-72
Boynukara, B., Gulhan, T., Alisarli, M., Gurturk, K., Solmaz, H.: Classical enterotoxigenic characteristics of Staphylococcus aureus strains isolated from bovine subclinical mastitis in Van, Turkey. Int. J. Food Microbiol., 2008; 125: 209-211.
Rall, V.L.M., Vieira, F.P., Rall, R., Vieitis, R.L., Fernades Jr., A. Candeias, J.M.G., Cardoso, K.F.G., Araujo Jr., J.P.: PCR detection of staphylococcal enterotoxin genes in Staphylococcus aurus strains isolated from raw and pasteurized milk. Vet. Microbiol., 2008; 132: 408-413.
Tranter, H.S.: Foodborne illness: foodborne staphylococcal illness. Lancet, 1996; 336: 1044-1046.
Mathieu, A.M., Isigidi, B.K., Devriese, L.A., Godard, C., Van Hoof, R.: Characterization of Staphylococcus aureus and Salmonella spp. strains isolated from bovine meat in Zaire. Int. J. Food Microbiol., 1991; 14:119-126.
Normanno, G., Firinu, A., Virgilio, S., Mula, G., Dambrosio, A., Poggiu, A., Decastelli, L., Mioni, R., Sucuota, S., Bolzoni, G., Di Giannatale, E., Salinetti, A.P., La Salandra, G., Bartoli, M., Zuccon, F., Pirino, T., Sias, S., Parisi, A., Quaglia, N.C., Celano, G.V.: Coagulase-positive staphylococci and Staphylococcus aureus in foods products marketed in Italy. Food Microbiol., 2005; 98: 73-79.
Serraino, A., Alberghini, L., Fontana, M.C., Annemuller, C., Rosmini, R.: Occurrence of enterotoxin genes and macrorestriction analysis of Staphylococcus aureus isolated from bovine mastitis and bulk-tank milk samples in Italy, An epidemiological study. Italian J. Anim. Sci., 2004; 3: 47- 53.
Stephen, R., Annemuller, C., Hassan, A.A., Lammler, Ch.: Characterization of enterotoxigenic Staphylococcus aureus strains isolated from bovine mastitis in north-east Switzerland. Vet. Microbiol., 2001; 78: 373-382.
Loncarevic, S., Jorgensen, H.J., Lovseth, A., Mathisen, T., Rorvik, L.M.: Diversity of Staphylococcus aureus enterotoxin types within single samples of raw milk and raw milk products. J. Appl. Microbiol., 2005; 98: 344-350.
Katsuda, K., Hata, E., Kobayashi, H., Kohmoto, M., Kawashima, K., Tsunemitsu, H., Eguchi, M.: Molecular typing of Staphylococcus aureus isolated from bovine mastitic milk on the basis of toxin genes and coagulase gene polymorphisms. Vet. Microbiol., 2005; 105: 301-305
Orden, J.A., Goyache, J., Hernandez, J., Domenech, A., Suarez, G., Gomez-Lucia, E.: Detection of enterotoxins and TSST-1 secreted by Staphylococcus aureus isolated from ruminant mastitis. Comparison of ELISA and immunoblot. J. Appl. Microbiol., 1992; 72: 486-489.