The Acute Toxicity of Ammonia on Tilapia (Oreochromis niloticus L.) Larvae and Fingerlings

The aim of the present study was to determine median lethal concentrations (LC 50) of unionised ammonia (NH3) for the larvae and fingerlings of Tilapia (Oreochromis niloticus L. 1758). The mean 48 h LC50 values were determined as 1.009 ± 0.02 mg/l for larvae and 7.40 ± 0.01 mg/l for the fingerlings. Larvae exposed to different concentrations of ammonia moved very rapidly, lost equilibrium in water and showed sideways swimming. In the case of the fingerlings, an increase in their movements, ventilation, convulsions, spiral swimming, efforts to swallow air from the surface of water, increase in the mucous secretion in the gills and on the body surface, haemorrhage in the gills, and darkening in the eye and on the skin were observed. The histological study showed hyperplasia and fusion of the lamella on the gills.

The Acute Toxicity of Ammonia on Tilapia (Oreochromis niloticus L.) Larvae and Fingerlings

The aim of the present study was to determine median lethal concentrations (LC 50) of unionised ammonia (NH3) for the larvae and fingerlings of Tilapia (Oreochromis niloticus L. 1758). The mean 48 h LC50 values were determined as 1.009 ± 0.02 mg/l for larvae and 7.40 ± 0.01 mg/l for the fingerlings. Larvae exposed to different concentrations of ammonia moved very rapidly, lost equilibrium in water and showed sideways swimming. In the case of the fingerlings, an increase in their movements, ventilation, convulsions, spiral swimming, efforts to swallow air from the surface of water, increase in the mucous secretion in the gills and on the body surface, haemorrhage in the gills, and darkening in the eye and on the skin were observed. The histological study showed hyperplasia and fusion of the lamella on the gills.

___

  • EPA (United States Environmental Protection Agency): 1998
  • Update of Ambient Water Quality Criteria for Ammonia. USA.
  • United States Environmental Protection Agency. 822-R-98- 008.52-107. 1998. 8.
  • Stickney, R. R.: Principles of Aquaculture. John Wiley & Sons,
  • New York. 1994. 155-162. 9.
  • Richardson, J.: Acute toxicity of ammonia to inanga (Galaxias
  • maculatus). New Zeal. J. Mar. Fresh., 1991; 25: 327-330.
  • Lawson, T.B.: Fundamentals of Aquacultural Engineering. Chapman-Hall, an International Thomson Publishing Company, New York. 1995; 2-40.
  • Wong, M.H.: Toxicity test of landfill leachate using Sarotherodon mossambicus (Freshwater Fish), Ecotox. Environ. Saf., 1989; 17: 149-156.
  • APHA (American Public Health Association): Standard Methods for the Examination of Water And Wastewater.14thEdition Washington DC., USA. 1975; 1-1134.
  • Reish, D.L., Oshida, P.S.: Manual of Methods in Aquatic Environment Research. Part 10. Short -Term Static Bioassays. FAO Fish Tech. Pap., 1987; 247: 17-33.
  • ISO.: Water Quality. Determination of the acute lethal toxicity of substances to A Freshwater Fish. Semi-Static Method. 1996; ISO 7346-2. 1-11.
  • Schäperclaus, W.: Fischkrankheiten. Akademie Verlag., Berlin. 1979; 842-864.
  • Crossmon, O.: A modification of Mallory’s connective tissue stain with a discussion all the principles involute. Anat. Rec., 1937; 69: 31-38.
  • Daud, S.K., Hasbollah, D., Law, A.T.: Effects of un-ionised ammonia on Red Tilapia (O. mossambicus X O. niloticus Hybrid) The Second International Symposium on Tilapia in Aquaculture. Bangkok. Thailand. 1988; 15: 411-413.
  • Arthur, J.W., Corlis, W. W., Allen, K. N., Hedtke, S. F.: Seasonal toxicity of ammonia to five fish and nine invertebrate species. Bull. Environ. Contam. Toxicol., 1987; 38, 324-331.
  • Mallett, M., Eddy, F.B.: Effect of ammonia on the early life stages of carp (Cyprinus carpio L.) and roach (Rutilus rutilus). In: Müller, R., Lloyd, R., Eds. Sublethal and Chronic Effects of Pollutants on Freshwater Fishes. Blackwell, Oxford, 1995; 339-352.
  • McCormick, J. H., Broderius, S.J., Fiandt, J.T.: Toxicity of ammonia to early life stages of the green sunfish Lepomis cyanellus. Environ. Pollut. (A), 1984; 36: 147-163.
  • Smart, G. R.: Investigations of the toxic mechanisms of ammonia to fish-gas exchange in rainbow trout (Salmo gairdneri) exposed to acutely lethal concentrations. J. Fish Biol., 1978; 12: 93-104.
  • Knoph, M.B.: Acute toxicity of ammonia to Atlantic salmon (Salmo salar) parr. Comp. Biochem. Physiol., 1992; 101: 275-282.
  • Kucuk, S.: The effect of diel un-ionised ammonia fluctuation on juvenile hybrid striped bass, channel catfish and tilapia. Master Thesis. Mississippi State University, Mississippi, USA. 1999.
  • Kirk, R.S., Lewis, J.W.: An evaluation of pollutant induced changes in the gills of rainbow trout using scanning electron microscopy. Environ. Technol. 1993; 14: 577-585.
  • Mitchell, S.J., Cech, J.J.: Ammonia caused gill damage in channel catfish (Ictalurus punctatus): confounding effects of residual chlorine. Can. J. Fish. Aquat. Sci., 1983; 40: 242-247.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK