Şahin ASLAN, Hakan KOCAMIŞ, Mümtaz NAZLI, Nurhayat GÜLMEZ

Immunohistochemical Distribution of Desmin and Vimentin in the Skin of Zavot Cattle

The distribution patterns of desmin and vimentin were determined in Zavot cattle skin using standard immunohistochemical techniques. Skin samples were collected from the different regions of the bodies. Vimentin-immunoreactivity was observed in the cells of subepidermal region, in the myoepithelial cells surrounding sebaceous and sweat glands, in the fibroblast, smooth muscle, endothelial cells of vessels and some hair follicles. Desmin-immunoreactivity was dense in the smooth muscle of arrectores pilorum muscles and bulbus pilorum of the some hair follicles. Desmin and vimentin immunoreactivities were not different among the various regions of Zavot cattle skin.

Anahtar Kelimeler:

Desmin, vimentin, skin, cattle

Immunohistochemical Distribution of Desmin and Vimentin in the Skin of Zavot Cattle

Keywords:

Desmin, vimentin, skin, cattle,

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Bavdek, S.V., Golob, Z., Vandijk, J., Dorrestein, GM., Fazarinc, G.: Vimentin and desmin-positive cells in the moulting budgerigar (Melopsittacus undulatus) skin. Anat. Histol. Embryol., 1997; 26: 173-178.
Kacem, K., Seylaz, J., Aubinean, P.: Differential processes of vascular smooth muscle cells of rats and rabbits: An immunofluorescence study of desmin and vimentin distribution. Histochem. J., 1996; 28: 53-61. 3. Lazarides, E.: Intermediate filaments as mechanical integrators of cellular space. Nature, 1980; 283: 248-250.
Rode, B., Bavdek, S.V., Lackovic, G., Fazarinc, G., Bidovec, A.: Immunohistochemical study of normal and mange (S. scabei var. rupicaprae) infested chamois (Rupicapra rupicapra L.) skin. Anat. Histol. Embryol., 1998; 27: 187-192.
Franke, W.W., Appelhans, B., Schmid, E., Freudenstein, C., Osborn, M., Weber, K.: Identification and characterization of epithelial cells in mammalian tissues by immunofluorescence microscopy using antibodies to prekeratin. Differentiation, 1979; 15: 7-25.
Farina, V., Ibba, M., Asole, A., Zedda, M., Bo, L.: Immunolocalization of cytokeratins in some freshly fixed bovine epithelia. Eur. J. Bas. Appl. Histochem., 1991; 35: 393-400. 7. Hsu, S.M., Raine, L., Fanger, H.: Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem., 1981; 29: 577-580.
Shu, S., Ju, G., Fan, L.: The glucose oxidase-DAB-nickel in peroxidase histochemistry of the nervous system. Neurosci. Lett., 1988; 85: 169-171.
Lazarides, E., Granger, B.L.: Preparation and assay of the intermediate filament proteins desmin and vimentin. Method. Enzymol., 1982; 85: 488-508.
Marbini, A., Gemignani, F., Bellanova, M.F., Guidetti, D., Ferrari, A.: Immunohistochemical localization of utrophin and other cytoskeletal proteins in skin smooth muscle in neuromuscular diseases. J. Neurol. Sci., 1996; 143: 156-160.
Virtanen, I., Kallajoki., M., Narvanen, O., Paranko, J., Thornell, L.E., Miettinen, M., Lehto, V.P.: Peritubular myoid cells of human and rat testis are smooth muscle cells that contain desmin-type intermediate filaments. Anat. Rec., 1986; 215: 10-20.
Narisawa, Y., Kohda, H.: Arrector pili muscles surround human facial vellus hair follicles. Br. J. Dermatol., 1993; 129: 138-139.
Gould, V.E., Koukoulis, G.K., Jansson, D.S., Nagle, R.B., Franke, W.W., Moll, R.: Coexpression patterns of vimentin and glial filament protein with cytokeratins in the normal, hyperplastic and neoplastic breast. Am. J. Pathol., 1990; 137: 1143-1155.
Zavizion, B., Politis, I., Gorewit, R.C.: Bovine mammary myoepithelial cells I. lsolation, culture and characterization. J. Dairy Sci., 1992; 75: 3367-3380.
Franke, W.W., Schmid, E., Freudenstein, C., Appelhans, B., Osborn, M., Weber, K., Keenan, T.W.: Intermediate-sized filaments of the prekeratin type in myoepithelial cells. J. Cell Biol., 1980; 84: 633-654.
Vos, J.H., Van Den Ingh, T.S.G.A.M., Misdorp, W., Molenbeek, R.F., Van Mil, F.N., Rutteman, G.R., Ivanyi, D., Ramaekers, F.C.S.: Imnunohistochemistry with keratin, vimentin, desmin and a-smooth muscle actin monoclonal antibodies in canine mammary gland; normal mammary tissue. Vet. Quart., 1993; 15: 102-107.
Heid, H.W., Moll, I., Franke, W.W.: Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles. Differentiation, 1988; 37: 137- 157.
Broekaert, D., Cornille, A., Edo, H., Leigh, I., Ramaekers, F., Van Muijen, G., Coucke, P., De Bersaques, J., Kluyskens, P., Gillis, E.: A comparative immunohistochemical study of cytokeratin and vimentin expression in middle ear mucosa and cholesteatoma, and in epidermis. Virchows Archiv. A. Pathol. Anat., 1988; 413: 39- 51.
Mahrle, G., Bolling, R., Osborn, M., Weber, K.: Intermediate filaments of the vimentin and prekeratin type in human epidermis. J. Invest. Dermatol., 1995; 81: 46-48.
Gabbiani, G., Schmid, E., Winter, S., Chaponnier, C., De Ckhastonay, C., Vandekerckhove, J., Weber, K., Franke, W.W.: Vascular smooth muscle cells: Predominance of vimentin filaments and a specific alpha-type actin. Proc. Natl. Acad. Sci. USA., 1981; 78: 298-302.