Isolation of Arcanobacterium (Actinomyces) pyogenes from Abscessed Cattle Kidney and Identification by PCR

In this study, the presence of Arcanobacterium spp., which causes a variety of purulent infections involving the skin, joints and visceral organs, was investigated in abscessed kidney samples of cattle. A total of 500 cattle were examined at postmortem and 100 samples with abscess were collected. Arcanobacterium pyogenes was isolated in 40 (40%) of the samples examined. DNA extracted from the isolates were amplified by Polymerase Chain Reaction (PCR) using specific primers derived from plo gene of A. pyogenes and all the isolates were determined to be positive by PCR. It was concluded that PCR employed in this study may be applied for identification of A. pyogenes.

Isolation of Arcanobacterium (Actinomyces) pyogenes from Abscessed Cattle Kidney and Identification by PCR

In this study, the presence of Arcanobacterium spp., which causes a variety of purulent infections involving the skin, joints and visceral organs, was investigated in abscessed kidney samples of cattle. A total of 500 cattle were examined at postmortem and 100 samples with abscess were collected. Arcanobacterium pyogenes was isolated in 40 (40%) of the samples examined. DNA extracted from the isolates were amplified by Polymerase Chain Reaction (PCR) using specific primers derived from plo gene of A. pyogenes and all the isolates were determined to be positive by PCR. It was concluded that PCR employed in this study may be applied for identification of A. pyogenes.

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  • Ramos, C.P., Foster, G., Collins, M.D.: Phylogenetic analysis of the genus Actinomyces based on 16S rRNA gene sequences: description of Arcanobacterium bernardiae comb. nov., and Arcanobacterium pyogenes comb. nov. Int. J. Syst. Bacteriol., 1997; 47: 46-53.
  • Lechtenberg, K.F., Nagaraja, T.G., Leipold, H.W., Chengappa, M.M.: Bacteriologic and histologic studies of hepatic abscesses in cattle. Am. J. Vet. Res., 1988; 49: 58-62. 6. Timoney, J.F., Gillespie, J.H., Scott, F.W., Barlough, J.E.: Hagan and Bruner’s Microbiology and Infectious Diseases of Domestic Animals. Cornell University Press, Ithaca, NY, 1988.
  • Barbour, E.K., Brinton, M.K., Caputa, A., Johnson, J.B., Poss, P.E.: Characteristics of Actinomyces pyogenes involved in lameness of male turkeys in north-central United States. Avian Dis., 1991; 35: 192-196.
  • Brinton, M.K., Schellberg, L.C., Johnson, J.B., Frank, R.K., Halvorson, D.A., Newman, J.A.: Description of osteomyelitis lesions associated with Actinomyces pyogenes infection in the proximal tibia of adult male turkeys. Avian Dis., 1993; 37: 259- 262.
  • Sokkar, S.M., Mohamed, M.A., Atawia, M.: Experimental induction of renal lesions in chickens. Berl. Munch. Tierarztl. Wschr., 1998; 111: 161-163.
  • Jootar, P., Gherunpong, V., Saitanu, K.: Corynebacterium pyogenes endocarditis. Report of a case with necropsy and review of the literature. J. Med. Assoc. Thailand, 1978; 61: 596-601.
  • Kotrajaras, R., Tagami, H.:Corynebacterium pyogenes. It’s pathogenic mechanism in leg ulcers in Thailand. Int. J. Dermatol., 1987; 26: 45-50
  • Hillerton, J.E., Bramley, A.J.: Infection following challenge of the lactating and dry udder of dairy cows with Actinomyces pyogenes and Peptostreptococcus indolicus. Br. Vet. J., 1989; 145: 148- 159.
  • Semambo, D.K.N., Ayliffe, T.R., Boyd, J.S., Taylor, D.J.: Early abortion in cattle induced by experimental intrauterine infection with pure cultures of Actinomyces pyogenes. Vet. Rec., 1991; 129: 12-16.
  • Ruder, C.A., Sasser, R.G., Williams, R.J., Ely, J.K., Bull, R.C., Butler, J.E.: Uterine infections in the postpartum cow. II. Possible synergistic effect of Fusobacterium necrophorum Corynebacterium pyogenes. Theriogenology, 1981; 15: 573-580 and
  • Palmer, M.V., Whipple, D.L.: Arcanobacterium pyogenes as a cause of fatal pleuropneumonia after capture and transport of white-tailed deer Odocoileus viginianus. J. Vet. Diagn. Invest., 1999; 11: 468-471
  • Foreyt, W.J., Jessup, D.A.: Fatal pneumonia of bighorn sheep following association with domestic sheep. J. Wldlf Dis., 1982; 18: 163-168.
  • Tolle, A., Franke, V., Reichmuth, J.: Corynebacterium pyogenes mastitis-Bacteriological aspects. Deut. Tierarztl. Woch., 1983; 90: 256-260.
  • Hinton, M.: Bovine abortion associated with Corynebacterium pyogenes. Vet. Bull., 1972; 42: 753.
  • Hommez, J., Devriese, L.A., Miry, C., Castryck, F.: Characterisation of 2 groups of Actinomyces like-bacteria isolated from purulent lesions in pigs. J. Vet. Med. Series B., 1991; 38: 575-580.
  • Funke, G., Bernard, K.A.: Coryneform Gram-positive rods. In: Manual of Clinical Microbiology, ed. Murray PR, 7th ed., ASM Press, Washington, D.C. 1999; 319-345.
  • Billington, S.J., Post, K.W., Jost, B.H.: Isolation of Arcanobacterium (Actinomyces) pyogenes from cases of feline otitis externa and canine cystidis. J. Vet. Diagn. Invest., 2002; 14: 159-162.
  • Natterman, H., Horsch, F.: The Corynebacterium pyogenes infection in cattle. I. Incidence of the pathogen. Arch. Exp. Veterinarmed., 1977; 31: 405-413.
  • Tzora, A., Leontides, L.S., Amiridis, G.S., Manos, G., Fthenakis, G.C.: Bacteriological and epidemiological findings during examination of the uterine content of ewes with the retention of fetal membranes. Theriogenology, 2002; 57: 1809-1817.
  • Muz, A., Eröksüz, H., Öngör, H., Ertafl, H.B., Kaya, A., Kalender, H.: Microbiological, serological and pathological investigations on the prescapular lymph nodes with abscesses from sheep in the abattoir of meat and fish organisation in Elazığ. Fırat Univ. J. Hlth. Sci., 1995; 9: 204-211.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK