Serotyping of Mannheimia haemolytica Strains Isolated from Pneumonic Lungs of Sheep in the Aydın Region of Turkey

This study was conducted to serotype isolates of Mannheimia haemolytica in the Aydın region of Turkey. A total of 200 lung samples showing pneumonia signs were obtained from slaughterhouses in the Aydın region. Twenty-four strains were isolated as Mannheimia haemolytica complex. Twenty-two strains were identified as Mannheimia haemolytica and 2 were as Pasteurella trehalosi for the first time in the region. The serotyping studies of strains isolated from pneumonic lung samples showed that Mannheimia haemolytica serotypes A1, A2, A6, A7, A8 and Pasteurella trehalosi serotype T4 were present in Aydın.

Anahtar Kelimeler:

Mannheimia haemolytica, Pasteurella trehalosi, isolation, identification, serotyping

Serotyping of Mannheimia haemolytica Strains Isolated from Pneumonic Lungs of Sheep in the Aydın Region of Turkey

Keywords:

Mannheimia haemolytica, Pasteurella trehalosi, isolation, identification, serotyping,

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Gilmour, N.J.L., Gilmour, J.S.: Pasteurellosis of Sheep. In: Adlam, C.F., Rutter, J.M., Eds. Pasteurella and Pasteurellosis. Academic Press, London, 1989; 223-262.
Biberstein, E.L., Gills, M., Knight, H.: Serological types of Pasteurella haemolytica, Cornell Vet., 1960; 50: 283-300.
Pegram, G.R., Roeder, P.L., Scot, J.M.: Two new serotypes of Pasteurella haemolytica from sheep in Ethiopia. Trop. Anim. Health Prod., 1979; 11: 29-30. 4. Fraser, J., Laird, S., Gilmour, N.J.L.: A new serotype (biotype T) of Pasteurella haemolytica. Res. Vet. Sci., 1982; 32: 127-128.
Younan, M., Fodor, L.: Characterization of a new Pasteurella haemolytica serotype (A17). Res. Vet. Sci., 1995; 58: 98.
Sneath, P.H.A., Stevens, M.: Actinobacillus rossii sp. nov., Actinobacillus seminis sp. nov., nom. rev. Pasteurella bettii sp. nov., Pasteurella lymphangitidis sp. Nov., Pasteurella mairi sp. nov., and Pasteurella trehalosi sp. nov. Int. J. Syst. Bacteriol., 1990; 40: 141-153.
Angen, Q., Aalbaek, B., Falsen, E., Olsen, J.E., Bisgaard, M.: Phenotypical relationship among strains classified with the ruminant (Pasteurella) haemolytica complex using quantitative evaluation of phenotypic data. Zbl. Bakt., 1997; 285: 459-479. 8. Angen, Q., Caugant, D.A., Olsen, J.E., Bisgaard, M.: Genotyping relationships among strains classified with the (Pasteurella) haemolytica complex as indicated by ribotyping and multilocus enzyme electrophoresis. Zbl. Bakt., 1997; 286: 333-354.
Angen, Q., Olsen, J.E., Bisgaard, M.: Further studies on the relationships among strains classified as taxon 15, taxon 18, taxon 20, (Pasteurella) granulomatis or the (Pasteurella) haemolytica complex in ruminants using quantitative evaluation of phenotypic data. Zbl. Bakt., 1997; 286: 317-332.
Angen, Q., Quirie, M., Donachie, W., Bisgaard, M.: Investigations on the species specificity of Mannheimia (Pasteurella) haemolytica serotyping. Vet. Microbiol., 1999; 65: 283-290.
Biberstein, E.L.: Biotyping and serotyping of Pasteurella haemolytica. In: Bergam, T., Norris J.R. (Eds.) Methods in Microbiology, Academic Press, London, 1978; 253-269.
Gilmour, N.J.L., Angus, K.W., Gilmour, J.S.: Pasteurellosis. In: Martin, W.B., Aitken, I.D. (Eds.), Diseases of Sheep. Blackwell, Oxford, 1991; 133-139.
Frank, G.H.: Serotypes of Pasteurella haemolytica in sheep in Midwestern United States. J. Am. Vet. Res., 1982; 43: 2035- 2037.
Prince, D.V., Clarke, J.K., Alley, M.R.: Serotypes of Pasteurella haemolytica from the respiratory tract of sheep in New Zealand. New Zealand Vet. Res., 1985; 33: 76-77.
Fodor, L., Varga, J.: Characterization of a New Serotype of Pasteurella haemolytica isolated in Hungary. Res. Vet. Sci., 1988; 44: 399.
Diker, K.S., Akan, M., Haziroğlu, R.: Antimicrobial susceptibility of Pasteurella haemolytica and Pasteurella multocida isolated from pneumonic ovine lungs. Vet. Rec., 1994; 134: 597-598.
Diker, K.S., Akan, M., Kaya, O.: Evaluation of immunogenicity of Pasteurella haemolytica serotypes in experimental models. Turk J. Vet. Anim. Sci., 2000; 24: 139-143.
Haziroglu, R., Diker, K.S., Gulbahar, M.Y., Akan, M., Guvenc, T.: Studies of the pathology and microbiology of pneumonic lungs of lambs. Dtsch. Tierarztl. Wschr., 1994; 101: 441-443.
Kaya, O., Kırkan, S.: Aydın bölgesindeki sağlıklı ve pnömoni flüpheli koyunlardan Pasteurella haemolytica’nın izolasyonu, biyotip tayini ve antibiyotiklere duyarlılıkları. Bornova Vet. Kont. Araflt. Enst. Derg., 1999; 24: 21-25.
Holt, J.G., Kreig, N.R., Sneath, P.H.A., Staley, J.T., Williams, S.T.: Bergey’s Manual of Determinative Bacteriology. Hensley W.R. Eds., Facultatively Anaerobic Gram-Negative Rods. Williams and Wilkins, Baltimore, 1994; 196, 282.
Koneman, E.W., Allen, S.D., Janda, W.M., Schreckenberger, P.C., Winn, W.C.Jr.: Color Atlas and Textbook of Diagnostic Microbiology (fifth edition). Andrew Allen Eds., Miscellaneous Fastidious Gram-Negative Bacilli. Lippincott-Raven, Philadelphia, 1997; 1307, 1371.
Hill, A.E., Lainson, F.A.: Survey of restriction-modification systems and transformation in Mannheimia haemolytica and Pasteurella trehalosi. Vet. Microbiol., 2003; 92: 103-109.
Shewan, P.E.: Pasteurella. In: Gyles, C.L., Thoen, C.O., Eds., Pathogenesis of Bacterial Infections in Animals. Iowa State University Press, 1986; 147-153.
Gilmour, N.J.L., Martin, W.B., Sharp, J.M., Thompson, D.A., Wells, P.W.: The development of vaccines against pneumonic pasteurellosis in sheep. Vet. Rec., 1979; 111: 15.
Prince, D.V., Clarke, J.K., Alley, M.R.: Serotypes of Pasteurella haemolytica from the respiratory tract of sheep in New Zeal. Vet. J., 1985; 33: 76-77.
Angen, Q., Ahrens, P., Bisgaard, M.: Phenotypic and genotypic characterization of Mannheimia (Pasteurella) haemolytica-like strains isolated from diseased animals in Denmark. Vet. Microbiol., 2002; 84: 103-114.
Fodor, L., Varga, J., Hajtos, I., Szemeredi, G.: Serotypes of Pasteurella haemolytica isolated from sheep, goats and calves. Zbl. Vetmed. B, 1984; 31: 466-469.
Güler, L., Baysal, T, Gündüz, K., Erganifl, O., Kaya, O., Orhan, G.: Koyun ve keçilerden izole edilen Pasteurella haemolytica sufllarının biyotip ve serotiplendirilmesi. Veterinarium, 1996; 7: 5-12.
Gündüz, K., Erganifl, O.: Pnömonili sığır akciğerlerinden izole edilen Pasteurella haemolytica sufllarının biyotiplendirilmesi ve serotiplendirilmesi. Veterinarium, 1998; 9: 11-19.
Gentry, M. J., Confer, A. W., Holland, S. G.: Comparison of the toxic and antigenic properties of single bovine isolates of Pasteurella haemolytica representing five serotypes and an untypable strain. Vet. Microbiol., 1988; 16: 351-367.