Seroprevalence of brucellosis in human, sheep, and cattle populations in Kırıkkale (Turkey)

Türkiye'de Kırıkkale'nin kırsal kesimlerinde insanlarda, koyun ve sığır topluluklarında Brusella serorpevalansı araştırıldı. Serum örnekleri 1436 kişiden elde edildi (% 28.41 erkek ve % 71,59 bayan). Toplumun % 3,2 (46) si Rose Bengal Plate testi (RBPT) ile ve % 3.0 (43)'ü standard tüp aglutinasyon testi (STAT) ile pozitif olarak sonuç verdi. RBPT ile pozitif olarak bulunan serumlar ELISA testi kullanılarak % 75 (33 kişi) Brucella ELISA IgG ile ve%25 (11 kişi) Brucella ELISA IgM ile pozitif olarak sonuç verdi. RBPT ile 301 sığırdan ve 503 koyundan olmak üzere toplam 804 serum örneğinin araştırılması sonucu % 47 (52) seropozitiflik gözlendi. RBPT Ne pozitif olarak tespit edilen tüm hayvanlar STAT ile de pozitif olarak sonuç verdi. 301 sığır ve 503 koyunda doğrulama testi olarak kullanılan Kompleman Fiksasyon Testi (CFT) ile seropozitiflik sırasıyla % 1 ve % 8 olarak bulundu.

Kırıkkale (Türkiye)' de insan, koyun ve sığır topluluklarında bruselloz seroprevalansı

The seroprevalence of Brucellosis in human, sheep, and cattle populations was investigated in rural regions of Kırıkkale in Turkey. Serum samples were obtained from 1436 humans, and 3.2% (46) of the population was found to be positive by Rose Bengal Plate test (RBPT) and 3.0% {43) gave positive results with the standard tube agglutination test (STAT). Immunoglobulin class analysis of RBPT-positve sera using the ELISA test gave positive results for 33 people (75%) with Brucella ELISA IgG and for 11 people (25%) with Brucella ELISA IgM. The evaluation of 804 serum samples, which were obtained from 301 cattle and 503 sheep, showed 6.47% (52) seropositivity with RBPT. Additionally, all animals were found to be positive with STAT. The seropositivity was 1% and 8 %. respectively, in serum samples obtained from 301 cattle and 503 sheep with the complement fixation test (CFT), which was used as the confirmatory test.

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Godfroid. J.. Cloeckaert. A.. Liautarû, J.P.. Kohler. S., Fretin. D.. Walravens, K., Garin-Bastuji. B.. Letesson. J.J.: From the discovery of the Malta fever's agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. Vet. Res., 2005; 36: 313-326.
Office International des Epizooties. Bovine brucellosis. In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Pans: 01E. 2004: 409-438.
Dhand. N.K., Gumoer, S., Singh. B.B.. Aradhana. Bali M.S.. Kumar, H„ Sharma. D.R.. Singh. J., Sandhu K.S.: A study on the epidemiology of brucellosis in Punjab (India) using Survey Toolbox. Rev. Sci. Tech., 2005; 24: 879-885.
Mousa, A.R., Elhag, K.M.. Khogali, M., Marafie, A.A.: The nature of human brucellosis in Kuwait: study of 379 cases. Rev. Infect. Dis.. 1988; 10:211-217.
Mainar-Jaime. R.C., Munoz, P.M., de Miguel, M.J.. Grillo. M.J., Marin, CM., Moriyon, L. Blasco, J.M.: Specificity dependence between serological tests for diagnosing bovine brucellosis in Brucella-free farms showing false positive serological reactions due to Yersinia enterocolitis 0:9. Can. Vet. J„ 2005; 46: 913-916.
Diaz, R.. Maravi-Poma. E.. Rivera. A.: Comparison of counterimmunoelectrophoresis with other serological tests in the diagnosis of human brucellosis. Bull. Wrld. Hlth. Org., 1976; 53: 417-424.
Cox, P.S.V.: A comparison of the rapid slide and standard tube agglutination tests for bruellosis. Trans. R. Soc. Trop. Med. Hyg., 1968:62:517-521.
Edwards, J.M.B., Tannahill, A.J.. Patricia Bradstreet. CM.: Comparison of the indirect fluorescent antibody test with agglutination, complement-fixation and Coomb's test for Brucell. antibody. J. Clin. Pathol., 1970; 23: 161-165.
Munoz, P.M., Marin, CM., Monreal. D., Gonzalez. D.. Garin-Bastuji. B.. Diaz, R„ Mainar-Jaime, R.C., Moriyon, 1., Blasco, J.M.: Efficacy of several serological tests and antigens for diagnosis of bovine brucellosis in the presence of false-positive serological results due to Yersinia enterocolitis 0:9. Clin. Diagn. Lab. Immunol.. 2005; 12: 141-151.
Gall. D., Nielsen, K.: Serological diagnosis of bovine brucellosis: a review of test performance and cost comparison. Rev. Sci. Tech., 2004;23:989-1002.
Çetin. E.T.. Coral, B.. Bilgiç, A., Bilgehan, H.: Determination of Brucellosis incidence in Turkey. Turk. J. Med. Sci., 1990; 14: 324-334.
Dogruer, M., Yılmaz. S.: Türkiye'de Bruselloz. Etlik Vet. Bakt. Enst. Derg. 1963; 2: 1-29.
Golem, SB.: Memleketimizdeki insan ve ehli hayvanlarda Brucella bakımından serolojik araştırma. Türk. Hıfsız. Tecr. Biol. Mec, 1943; 1: 105-116.
Iyisan, A.Ş.. Akmaz, Ö.: Türkiye'de sığır ve koyunlarda brucellosis'in seroepidemiyolojısi. Pendik Vet. Mikrobiyol. Derg., 2000;31:21-75.
Abernethy, D.A., Pfeiffer. D.U., Watt. R., Denny. CO., McCullough, S., McDowell, S.W.: Epidemiology of bovine brucellosis in Northern Ireland between 1990 and 2000. Vet. Rec.,2006; 158:717-721.
Erdenebaatar. J., Bayarsaikhan. B., Yondondorj, A., Watarai. M., Shirahata, T., Jargalsaikhan, E., Kawamoto. K,. Makino, S.: Epidemiological and serological survey of brucellosis in Mongolia by ELISA using sarcosine extracts. Microbiol. Immunol., 2004; 48:571-577.
Barbuddhe, S.B.. Yadava, V.K., Singh. D.K.: Detection of IgM and IgG antibodies against Brucella by dot-ELISA in humans. J. Commun. Dis.. 1994; 26: 1-5.
Colmenero, J.D., Porras, J.. Cardenas, A., Ocon. P., Reguera, J.M., Delgado, M„ Sedeno, J.: Evaluation of the chromottre EIA test in the diagnosis of human brucellosis. Enferm. Infect. Microbiol. Clin., 1994; 12: 60-65.
Gazapo, E., Gonzalez Lahoz, J., Subiza J.L. Baquero M., Gil, J., de la Concha. E.G.: Changes in IgM and IgG antibody concentrations in brucellosis over time: importance for diagnosis and follow-up. J. Infect. Dis., 1989: 159: 219-225.
Baum, M„ Zamir. 0.. Bergman-Rios. R.. Katz, E., Beider, Z., Cohen. A.. Banai, M.: Comparative evaluation of microagglutination test and serum agglutination test as supplementary diagnostic methods for brucellosis. J. Clin. Microbiol., 1995; 33: 2166-2170.