A seroepidemiological study of canine leishmaniasis was carried out in 220 healthy pet dogs from 11 different regions in Bulgaria allocated in 5 regions in southern Bulgaria: Plovdiv (n = 20), Stara Zagora (n = 20), Yambol (n = 20), Burgas (n = 20), and Blagoevgrad (n = 20), and 6 regions in northern Bulgaria: Varna (n = 20), Silistra (n = 20), Rousse (n = 20), Veliko Tarnovo (n = 20), Pleven (n = 20), and Montana (n = 20). The indirect fluorescent antibody test was used. All blood sera examined yielded negative results for leishmaniasis, thus suggesting that healthy household dogs in Bulgaria are not infected by Leishmania.

A seroepidemiological study of canine leishmaniasis was carried out in 220 healthy pet dogs from 11 different regions in Bulgaria allocated in 5 regions in southern Bulgaria: Plovdiv (n = 20), Stara Zagora (n = 20), Yambol (n = 20), Burgas (n = 20), and Blagoevgrad (n = 20), and 6 regions in northern Bulgaria: Varna (n = 20), Silistra (n = 20), Rousse (n = 20), Veliko Tarnovo (n = 20), Pleven (n = 20), and Montana (n = 20). The indirect fluorescent antibody test was used. All blood sera examined yielded negative results for leishmaniasis, thus suggesting that healthy household dogs in Bulgaria are not infected by Leishmania.