Fuat AYDIN, K. Semih GÜMÜŞSOY, Tuba İÇA, Bülent SÜMERKAN, Duygu EŞEL, Mehmet AKAN

Kayseri’de (Türkiye) Çeşitli Kaynaklarda Campylobacter jejuni’nin Prevalansı ve İzole Edilen Suşların PCR-RFLP ile Moleküler Analizi

Bu çal›flma Kayseri yöresinde çeflitli kaynaklardan Campylobacter jejuni’nin izolasyonu, identifikasyonu ve identifiye edilen sufllar›n genetik olarak tiplendirilmesi amac›yla yap›ld›. Çal›flma kapsam›nda, 5167 insan d›flk› svab›, 600 adet köpek rektal svab›, 600 adet s›¤›r safra kesesi ve 300 adet (paket) tavuk eti olmak üzere toplam 6667 adet numune incelendi. Örnekler izolasyon amac›yla mCCDA (cefoperazone charcoal desoxycholate agar)’a ekildi. C. jejuni’n›n identifikasyonunda fenotipik testler ve PCR (Polymerase Chain Reaction)’den yararlan›ld›. ‹nsan, köpek, s›¤›r ve tavuk örneklerinden s›ras›yla % 1,43, % 43,50, % 31,16 ve % 56 oranlar›nda C. jejuni izole edildi. Çal›flma periyodu boyunca izole edilen 690 adet C. jejuni suflu içerisinden 200 adet seçilerek PCR-RFLP, (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) fla-typing yöntemi ile tiplendirildi. Moleküler tiplendirme amac› ile DdeI ve HinfI restriksiyon enzimleri kullan›ld›. DdeI enzimi ile muameleden sonra sufllar 4 ile 7 aras›nda band oluflturdu ve incelenen tüm sufllar 20 farkl› band profili gösterdi. Farkl› kaynaklardan izole edilen sufllar aras›nda benzer band profili veren sufllara rastlanmad›. Bu çal›flmada HinfI enziminin sufllar›n tiplendirilmesinde ay›r›c› olmad›¤› belirlendi.

Anahtar Kelimeler:

‹nsan, köpek, tavuk, s›¤›r, Campylobacter jejuni, PCR-RFLP (fla-typing)

The Prevalence of Campylobacter jejuni in Various Sources in Kayseri, Turkey, and Molecular Analysis of Isolated Strains by PCR-RFLP

The objective of this study was to isolate, identify, and genotype Campylobacter jejuni from various sources in the province of Kayseri, Turkey. A total of 6667 samples consisting of 5167 human fecal swabs, 600 dog rectal swabs, 600 cattle gallbladders, and 300 chicken carcasses were examined. The samples were plated onto mCCDA (cefoperazone charcoal desoxycholate agar) agar. In order to identify C. jejuni, phenotypic tests and PCR (polymerase chain reaction) were performed. C. jejuni was isolated in 1.43%, 43.50%, 31.16%, and 56% of the human, dog, cattle, and chicken samples, respectively. Among the 690 C. jejuni strains that were isolated during the study period, 200 C. jejuni strains (50 strains from each species) were randomly selected. The selected strains were typed by using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) fla-typing. DdeI and HinfI restriction enzymes were used for molecular typing. Following the DdeI enzyme application, the strains produced various numbers of bands between 4 and 7, with a total of 20 different band profiles. No similar band profiles were seen among the strains isolated from different sources. It was found that HinfI was not a more discriminative enzyme for fla-typing of C. jejuni isolates.

Keywords:

Human, dog, chicken, cattle, Campylobacter jejuni, PCR-RFLP (fla-typing),

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