Relations between electrical conductivity, somatic cell count, California mastitis test and some quality parameters in the diagnosis of subclinical mastitis in dairy cows

This study was conducted to determine the effectiveness of the electrical conductivity (EC) method on the diagnosis of subclinical mastitis in dairy cows comparing with somatic cell count (SCC) and California mastitis test (CMT), and also to investigate the effect of these values on the amount of total viable bacteria, density, freezing point, and mineral substances. A total of 386 milk samples collected from quarters of 188 cows at 10 different farms were used as materials. Of the samples, 258 (66.85%) were CMT (+), 85 (22.02%) were CMT (++), and 43 (11.13%) were CMT (+++). The mean EC and SCC were 25.71, 28.02, and 29.63 mS and 249,453, 1,167,058, and 2,108,139, respectively, according to the CMT results. The total viable bacteria counts ranged from 3.4771 to 6.9395, from 3.4771 to 7.3617, and from 4.7782 to 7.5315 log CFU/mL, respectively. The average amounts of mineral substances were 0.65%, 0.70%, and 0.76%; the density values were 1.031, 1.0297, and 1.029; and the freezing points were -0.5281, -0.5285, and -0.5282, respectively. As a result, it has been concluded that EC showed similarity with the CMT and the SCC in the detection of subclinical mastitis; furthermore, its reliability would further increase when used together with the other diagnostic methods.

Relations between electrical conductivity, somatic cell count, California mastitis test and some quality parameters in the diagnosis of subclinical mastitis in dairy cows

This study was conducted to determine the effectiveness of the electrical conductivity (EC) method on the diagnosis of subclinical mastitis in dairy cows comparing with somatic cell count (SCC) and California mastitis test (CMT), and also to investigate the effect of these values on the amount of total viable bacteria, density, freezing point, and mineral substances. A total of 386 milk samples collected from quarters of 188 cows at 10 different farms were used as materials. Of the samples, 258 (66.85%) were CMT (+), 85 (22.02%) were CMT (++), and 43 (11.13%) were CMT (+++). The mean EC and SCC were 25.71, 28.02, and 29.63 mS and 249,453, 1,167,058, and 2,108,139, respectively, according to the CMT results. The total viable bacteria counts ranged from 3.4771 to 6.9395, from 3.4771 to 7.3617, and from 4.7782 to 7.5315 log CFU/mL, respectively. The average amounts of mineral substances were 0.65%, 0.70%, and 0.76%; the density values were 1.031, 1.0297, and 1.029; and the freezing points were -0.5281, -0.5285, and -0.5282, respectively. As a result, it has been concluded that EC showed similarity with the CMT and the SCC in the detection of subclinical mastitis; furthermore, its reliability would further increase when used together with the other diagnostic methods.

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  • 1. Dürr, J.W., Cue, R.I., Monardes, H.G., Moro-Méndez, J., Wade, K.M.: Milk losses associated with somatic cell counts per breed, parity and stage of lactation in Canadian dairy cattle. Livestock Sci., 2008; 117: 225-232.
  • 2. Nielen, M., Schukken, Y.H., Van De Broek, J., Brand, A., Deluyker, H.A., Maatje, K.: Relations Between On-Line Electrical Conductivity and Daily Milk Production on a Low Somatic Cell Count Farm. J. Dairy Sci., 1993; 76: 2589-2596.
  • 3. Atasever, S., Erdem H.: Association between subclinical mastitis markers and body condition scores of Holstein cows in the Black Sea region, Turkey. J. Anim. Vet. Adv., 2009; 8: 476-480.
  • 4. Skrzypek, R., Wójtowski, J. Fahr, R.D.: Factors affecting somatic cell count in cow bulk tank milk--a case study from Poland. J. Vet. Med. Series A, 2004; 51: 127-131.
  • 5. Baştan, A., Kaymaz, M., Findik, M., Erünal, N.: The use of electrical conductivity, somatic cell count and california mastitis test in diagnosis of subclinical mastitis in dairy cows. Ank. Univ. Vet. Fak. Derg., 1997; 44: 1-6.
  • 6. Lafi , S.Q.: Use of somatic cell counts and California Mastitis Test results from udder halves milk samples to detect subclinical intramammary infection in Awassi sheep. Small Rum. Res., 2006; 62: 83-86.
  • 7. Kitchen, B.J.: Review of the progress of dairy science: bovine mastitis: milk compositional changes and related diagnostic tests. J. Dairy Res., 1981; 48: 167-172.
  • 8. National Mastitis Council (NMC): Microbiological procedures for the diagnosis of udder infection. 3rd ed. Arlington, VA: National Mastitis Council Inc. 1990.
  • 9. Schalm, O., Noorlander, D.: Experiments and observations leading to the development of California mastitis test. JAVMA, 1957; 130: 199-204.
  • 10. Sierra, D., Sanchez, A., Luengo, C., Corrales, J.C., Morales, C.T., Contreras, A., Gonzalo, C.: Temperature effects on Fossomatic cell counts in goat milk. Int. Dairy J., 2006; 16, 385-387.
  • 11. Harrigan, W.F.: Laboratory Methods in Food Microbiology. Academic Press, London. 1998.
  • 12. Nielen, M., Deluyker, H., Schukken, Y.H., Brand, A.: Electrical conductivity of milk: measurement, modifiers, and meta analysis of mastitis detection performance. J. Dairy Sci., 1992; 75: 606-614.
  • 13. Midleton, J.R., Hardin, D., Steevens, B., Randle, R., Tyler, J.W.: Use of somatic cell counts and California mastitis test results from individual quarter milk samples to detect subclinical intramammary infection in dairy cattle from a herd with a high bulk tank somatic cell count. JAVMA, 2004; 224: 419-423.
  • 14. Dingwell, R.T., Leslie, K.E., Schukken, Y.H., Sargeant, J.M., Timms, L.L.: Evaluation of the California mastitis test to detect an intramammary infection with a major pathogen in early lactation dairy cows. Can. Vet. J., 2003; 44: 413-416.
  • 15. Rişvanlı, A., Kalkan, C.: The effect of age and breed on somatic cell count and microbiological isolation rates in milk of dairy cows with subclinical mastitis. YYÜ. Vet. Fak. Derg,. 2002; 13: 84-87. (in Turkish with summary in English).
  • 16. Baştan, A., Kaçar, C., Acar, D.B., Şahin, M., Cengiz, M.: Investigation of the incidence and diagnosis of subclinical mastitis in early lactation period cows. Turk. J. Vet. Anim. Sci., 2008; 32: 119-121.
  • 17. Bhutto, A.L., Murray, R.D., Woldehiwet, Z.: California mastitis test scores as indicators of subclinical intra-mammary infections at the end of lactation in dairy cows. Res. Vet. Sci., 2010, doi:10.1016/j.rvsc.2010.10.006
  • 18. Kuplulu, S., Vurali, R., Izgür, H., Kılıçoğlu, Ç., Baştan, A., Kayzmaz, M., Erdeger, J.: Subklinik mastitislerin tanısında “milk checker” ın kullanılması. Ank. Univ. Vet. Fak. Derg., 1995; 42: 281-284.
  • 19. Norberg, E., Hogeveen, H., Korsgaard, I.R., Friggens N.C., Sloth, K.H.M.N., Løvendahl, P.: Electrical conductivity of milk: ability to predict mastitis status. J. Dairy Sci., 2004; 87: 1099- 1107.
  • 20. Tekeli, T., Semecan, A., Işık, M.K.: Subklinik mastitislerin tanısmda pratik bir yöntem. Hay. Araş. Derg., 1993; 3: 62-65.
  • 21. Bruckmaier, R.M., Ontsouka, C.E., Blum, J.W.: Fractionized milk composition in dairy cows with subclinical mastitis. Vet. Med. - Czech., 2004; 49: 283-290.
  • 22. Ilie, L.I., Tudor, L., Galis, A.M.: The electrical conductivity of cattle milk and the possibility of mastitis diagnosis in Romania. Vet. Med. Sci. Works, 2010; 43: 220-227.
  • 23. Janzekovic, M., Brus, M., Mursec, B., Vinis, P., Stajnko, D., Cus, F.: Mastitis detection based on electric conductivity of milk. JAMME, 2009; 34: 39-46.
  • 24. Matei, S.T., Ioan, G., Liviu, B., Ciupe, S., Mihai, B.: Comparative studies on the subclinical mastitis diagnosis and physicochemical changes in mastitis cow milk. 1st EMUNI Research Souk 2009 (EMUNI ReS 2009), 9 June 2009, Romania.
  • 25. Wendorff , W.L., Kraiser, R.M., Bradley, R.L.: Freezing point impacts on quality premiums. UW Dairy Alert, Dec., 1993; 1: 1-8.
  • 26. Mitchell, G.E.: The contribution of lactose, chloride, citrate and lactic acid to the freezing point of milk. Aust. J. Dairy Technol., 1989; 44: 61-64.
  • 27. El Zubeir, E.M.I., El Owni O.A.O., Mohamed, G.E.: Correlation of minerals and enzymes in blood serum and milk of healthy and mastitic cows. Res. J. Agri. Bio. Sci., 2005; 1: 45-49.
  • 28. Ogola, H., Shitandi, A., Nanua, J.: Effect of mastitis on raw milk compositional quality. J. Vet. Sci., 2007; 8: 237-242.
  • 29. Pyörälä, S.: Indicators of infl ammation in the diagnosis of mastitis. Vet. Res., 2003; 34: 565-578.
  • 30. Shitandi, A., Ogollah, H., Nanua, J.N.: Effect of subclinical mastitis on milk composition in the Kenyan smallholder dairy herds. ACSC Proceedings, 2005; 7, 545-550.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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