Güven KAŞIKÇI, Ömer ÇETİN, Enver Barış BİNGÖL, Mehmet Can GÜNDÜZ

İneklerde subklinik mastitis tanısında elektrik iletkenliği, somatik hücre sayısı, Kaliforniya mastitis testi ve bazı kalite parametreleri arasındaki ilişkiler

Bu araştırmada ineklerde subklinik mastitis tanısında kullanılan elektriksel iletkenlik (EC) yönteminin etkinliğinin, somatik hücre sayısı (SCC) ve Kaliforniya Mastitis Testi (CMT) ile karşılaştırarak araştırılması; aynı zamanda bu değerlerin toplam canlı mikroorganizma sayısı, yoğunluk, donma noktası ve mineral madde miktarı üzerine etkilerinin belirtilmesi amaçlanmıştır. Çalışmada 10 farklı çift likte bulunan 188 ineğin toplam 386 meme lobundan alınan süt örnekleri materyal olarak kullanılmıştır. Bu örneklerin 258’i (% 66,85) CMT (+), 85’i (% 22,02) CMT (++) ve 43’ü (% 11,13) CMT (+++) olarak değerlendirilmiştir. CMT sonuçlarına göre ortalama elektriksel iletkenlik değerleri sırasıyla 25,71, 28,02 ve 29,63 mS ve ortalama somatik hücre sayıları ise sırasıyla 249.453, 1.167.058 ve 2.108.139 olarak belirlenmiştir. Toplam canlı mikroorganizma sayısı sırasıyla 3,4771-6,9395, 3,4771-7,3617 ve 4,7782-7,5315 Log CFU/ mL olarak bulunmuştur. Mineral madde miktarı ortalama olarak sırasıyla % 0,65, % 0,70 ve % 0,76; yoğunluk 1,031, 1,0297 ve 1,029 ve donma noktası ise –0,5281, –0.5285 ve –0,5282 olarak saptanmıştır. Sonuç olarak, subklinik mastitisli sütlerde elektriksel iletkenliğin saptanmasının CMT ve SCC ile benzerlik gösterdiği; dolayısıyla subklinik mastitislerin teşhisi için kullanılabilir bir yöntem olabileceği; ayrıca diğer tanı yöntemleri ile kullanıldığında güvenilirliğinin daha da artacağı kanısına varılmıştır.

Anahtar Kelimeler:

Süt, elektrik iletkenliği, somatik hücre sayısı, Kaliforniya mastitis testi, subklinik mastitis

Relations between electrical conductivity, somatic cell count, California mastitis test and some quality parameters in the diagnosis of subclinical mastitis in dairy cows

This study was conducted to determine the effectiveness of the electrical conductivity (EC) method on the diagnosis of subclinical mastitis in dairy cows comparing with somatic cell count (SCC) and California mastitis test (CMT), and also to investigate the effect of these values on the amount of total viable bacteria, density, freezing point, and mineral substances. A total of 386 milk samples collected from quarters of 188 cows at 10 different farms were used as materials. Of the samples, 258 (66.85%) were CMT (+), 85 (22.02%) were CMT (++), and 43 (11.13%) were CMT (+++). The mean EC and SCC were 25.71, 28.02, and 29.63 mS and 249,453, 1,167,058, and 2,108,139, respectively, according to the CMT results. The total viable bacteria counts ranged from 3.4771 to 6.9395, from 3.4771 to 7.3617, and from 4.7782 to 7.5315 log CFU/mL, respectively. The average amounts of mineral substances were 0.65%, 0.70%, and 0.76%; the density values were 1.031, 1.0297, and 1.029; and the freezing points were -0.5281, -0.5285, and -0.5282, respectively. As a result, it has been concluded that EC showed similarity with the CMT and the SCC in the detection of subclinical mastitis; furthermore, its reliability would further increase when used together with the other diagnostic methods.

Keywords:

Key words: Milk, electrical conductivity, somatic cell count, California mastitis test, subclinical mastitis,

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