Iraz AKIŞ AKAD, Ahmet MENGİ, Kemal Özdem ÖZTABAK

Doğu Anadolu Kırmızısı, Güney Anadolu Kırmızısı ve Boz ırk sığırlarda büyüme hormonu reseptör geni polimorfizmlerinin belirlenmesi

Bu çalışmada Türkiye’deki yerli sığır ırklarından Güney Anadolu Kırmızısı (GAK), Doğu Anadolu Kırmızısı (DAK) ve Boz ırk sığırlarda, büyüme hormonu reseptör (GHR) genindeki polimorfi zmlerin genotip ve allel dağılımlarının belirlenmesi amaçlanmıştır. Her sığır ırkından 50’er adet bireyden kan alınarak standart amonyum asetatla çöktürme yöntemiyle DNA örnekleri izole edilmiştir. Hedef bölgeler polimeraz zincir reaksiyonuyla çoğaltılmış ve AluI, AccI, StuI, NsiI ve Fnu4HI restriksiyon enzimleri ile sindirilmiştir. Süt verimiyle ilişkili GHR/AluI polimorfi zmi + allelinin frekansı Boz ırk sığırlarda, et verimiyle ilişkili GHR/AluI polimorfi zmi – allelinin frekansı GAK ve DAK ırkı sığırlarda düşük bulunmuştur. Süt verimini etkileyen GHR/StuI polimorfi zmi + allelinin frekansı DAK ırkında düşük, GHR/NsiI polimorfi zmi –/– genotipi ise üç ırkta da düşük olarak tespit edilmiştir. Et verimiyle ilişkili GHR/Fnu4HI polimorfi zmi + allelinin frekansı GAK ve DAK ırklarında düşük bulunmuştur. Sonuç olarak GHR genindeki verim özellikleri açısından etkili bazı allellerin yerli ırklarda yüksek verimli Avrupa ırklarına kıyasla daha düşük frekanslara sahip olduğu belirlenmiştir.

Anahtar Kelimeler:

PZR-RFLP, GHR geni, SNP, Doğu Anadolu Kırmızısı, Güney Anadolu Kırmızısı, Boz ırk

A determination of growth hormone receptor gene polymorphisms in East Anatolian Red cattle, South Anatolian Red cattle, and Turkish Grey cattle

The objective of this study was to determinate the genotype and allele distributions of growth hormone receptor gene polymorphisms in East Anatolian Red cattle (EAR), South Anatolian Red cattle (SAR), and Turkish Grey (TG) cattle. To this end, 50 EAR, 50 SAR, and 50 TG cattle were used in the study. DNA samples were isolated by using the standard ammonium acetate salt-out method. Target regions were amplified and digested by AluI, AccI, StuI, NsiI, and Fnu4HI restriction enzymes. The + allele frequency of growth hormone receptor (GHR)/AluI polymorphism related with milk traits in TG cattle and the - allele frequencies of GHR/AluI polymorphism related with meat traits in EAR and SAR cattle were found to be low. The + allele frequency of GHR/StuI polymorphism affecting milk traits was found to be low in the EAR breed. The -/- genotype of GHR/NsiI polymorphism was also low in all 3 of the breeds. The + allele frequency of GHR/Fnu4HI polymorphism related with meat traits turned out to be low in the SAR and EAR breeds. In conclusion, it can be said that some of the alleles of GHR polymorphisms that are favorable for trait qualities occur less frequently in SAR, EAR, and TG cattle breeds than they do in high-trait European breeds.

Keywords:

Key words: PCR-RFLP, GHR gene, SNP, East Anatolian Red cattle, South Anatolian Red cattle, Turkish Grey cattle,

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