Turan KARACA, Adem KARA, Nejdet ŞİMŞEK, Sema USLU, Deniz TEKİNER, Mecit YÖRÜK

Immunohistochemical distribution of glucagon -, insulin -, somatostatin -, gastrin-,and serotonin-containing cells in the pancreas of the Van cat

The regional distribution, relative frequency, and appearance of glucagon (A-cell)-, insulin (B-cell)-, somatostatin (D-cell)-, gastrin (G-cell)-, and serotonin (EC-cell)-secreting cells in the endocrine and exocrine pancreas of Van cats were examined using the immunohistochemistry method. Glucagon immunopositive A-cells were principally found in the central region of the islets of Langerhans, while insulin immunopositive B-cells were located in the periphery of the pancreatic islets. Moreover, several A- and B-cells were observed as only single cells or clusters of 2 to 3 immunopositive cells in the exocrine parenchyma and the pancreatic duct epithelium. Somatostatin and gastrin immunopositive reactivities were negligible in the peripheral regions of the pancreatic islets of Langerhans and in the exocrine parenchyma. However, serotonin-immunopositive EC-cells were observed in neither the endocrine islets nor any other sites of the tissue. The existence, regional distribution, and relative frequency of A-, B-, D-, G- and EC-cells in the pancreas of Van cats have been analyzed in this study for the first time. The immunopositivity and distribution of endocrine cells in the Van cat pancreas were determined to be partially different from those of other carnivorous species such as dogs and other cats.

Anahtar Kelimeler:

immunohistochemistry, Langerhans islet, Van cat pancreas, Endocrine cell

Immunohistochemical distribution of glucagon -, insulin -, somatostatin -, gastrin-, and serotonin-containing cells in the pancreas of the Van cat

Keywords:

immunohistochemistry, Langerhans islet, Van cat pancreas, Endocrine cell,

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Kocamis H, Karadag Sari E, Nazli M, Gulmez N, Aslan S, Deprem T. Immunohistochemical distribution of insulin-, glucagon-, and somatostatin-containing cells in the pancreas of the rat (Wistar albino). Kafkas Univ Vet Fak Derg 2009; 15: 611–614.
Cabrera O, Berman M, Kenyon NS, Ricordi C, Berggren P, Caicedo A. The unique cytoarchitecture of human pancreatic islets has implications for islet cell function. P Natl Acad Sci USA 2006; 103: 2334–2339.
Elayat AA, El-Naggar MM, Tahir M. An immunocytochemical and morphometric study of the rat pancreatic islets. J Anat 1995; 186: 629–637.
Furuoka H, Ito H, Hamada M, Suwa T, Satoh H, Itakura C. Immunocytochemical component of endocrine cells in pancreatic islets of horses. Jpn J Vet Sci 1989; 51: 35–43.
Furuzawa Y, Ohmori Y, Watanabe T. Immunohistochemical morphometry of pancreatic islets in the cat. J Vet Intern Med 1992; 54: 1165–1173.
Furuzawa Y, Ohmori Y, Watanabe T. Immunohistochemical studies of neural elements in pancreatic islets of the cat. J Vet Intern Med 1996; 58: 641–646.
Muranishi T, Takehana K, Hiratsuka T, Kobayashi A, Eerdunchaolu Iwasa K, Abe M. An investigation of the relationship between duct system and A cell-rich and PP cellrich pancreatic islets in the canine pancreas. J Vet Intern Med 1999; 61: 737–742.
O’Brien TD, Hayden DW, Johnson KH, Fletcher TF. Immunohistochemical morphometry of pancreatic endocrine cells in diabetic, normoglycemic glucose-intolerant and normal cats. J Comp Pathol 1986; 96: 357–369.
Kim A, Miller K, Jo J, Kilimnik G, Wojcik P, Hara M. Islet architecture: a comparative study. Islets 2009; 1: 129–136.
Kim H, Toyofuku Y, Lynn FC, Chak E, Uchida T, Mizukami H, Fujitani Y, Kawamori R, Miyatsuka T, Kosaka Y et al. Serotonin regulates pancreatic beta cell mass during pregnancy. Nat Med 2010; 16: 804–808.
Bayrakdar A, Yaman M, Atalar O, Gencer Tarakci B, Ceribasi S. Distribution of neuropeptides in endocrine and exocrine pancreas of long-legged buzzard (Buteo rufinus): an immunohistochemical study. Regul Pept 2011; 166: 121–127.
Gustavsen CR, Pillay N, Heller RS. An immunohistochemical study of the endocrine pancreas of the African ice rat, Otomys sloggetti robertsi. Acta Histochem 2008; 110: 294–301.
Kanter M, Uysal H, Karaca T, Sagmanligil HO. Depression of glucose levels and partial restoration of pancreatic beta-cell damage by melatonin in streptozotocin-induced diabetic rats. Arch Toxicol 2006; 80: 362–369.
Paulmann N, Grohmann M, Voigt JP, Bert B, Vowinckel J, Bader M, Skelin M, Jevsek M, Fink H, Rupnik M et al. Intracellular serotonin modulates insulin secretion from pancreatic B-cells by protein serotonylation. PLoS Biol 2009; 7: e1000229.
Ponery AS, Adeghate E. Distribution of NPY and SP and their effects on glucagon secretion from the in vitro normal and diabetic pancreatic tissues. Peptides 2000; 21: 1503–1509.
Wieczorek G, Pospischil A, Perentes E. A comparative immunohistochemical study of pancreatic islets in laboratory animals (rats, dogs, minipigs, nonhuman primates). Exp Toxicol Pathol 1998; 50: 151–172.
Şimşek N, Özüdoğru Z, Alabay B. Immunohistochemical studies on the splenic lobe of the pancreas in young Japanese quails (Coturnix c. japonica). Deut Tierärztl Woch 2008; 115: 189–193. Steiner DJ, Kim A, Miller K. Pancreatic islet plasticity: interspecies comparison of islet architecture and composition. Islets 2010; 2: 135–145.
Sánchez A, Celani S, Lawzewitsch IV. Pancreatic islets in Platyrrhini monkeys: Callithrix jacchus, Saimiri boliviensis, Aotus azarae and Cebus apalla. A cytological and immunocytochemical study. Primates 1991; 32: 93–103.
Reddy S, Bibby NJ, Elliott RB. Cellular distribution of insulin, glucagon, pancreatic polypeptide hormone and somatostatin in the fetal and adult pancreas of the guinea pig: a comparative immunohistochemical study. Eur J Cell Biol 1985; 38: 301–305.
Simsek N, Kaya M, Kara A, Can I, Karadeniz A, Kalkan Y. Effects of melatonin on islet neogenesis and beta cell apoptosis in streptozotocin-induced diabetic rats: an immunohistochemical study. Domest Anim Endocrinol 2012; 43: 47–57.
Rooman I, Lardon J, Bouwens L. Gastrin stimulates beta-cell neogenesis and increases islet mass from transdifferentiated but not from normal exocrine pancreas tissue. Diabetes 2002; 51: 686–690.
Wang TC, Bonner-Weir S, Oates PS, Chulak MB, Simon B, Merlino GT, Schmidt EV, Brand SJ. Pancreatic gastrin stimulates islet differentiation of transforming growth factor alpha-induced ductular precursor cells. J Clin Invest 1993; 92: 1349–1356.
Sonda S, Silva AB, Grabliauskaite K, Saponara E, Weber A, Jang JH, Züllig RA, Bain M, Graf TR, Hehl AB et al. Serotonin regulates amylase secretion and acinar cell damage during murine pancreatitis. Gut 2013; 62: 890–898.
Schraenen A, Lemaire K, de Faudeur G, Hendrickx N, Granvik M, Van Lommel L, Mallet J, Vodjdani G, Gilon P, Binart N et al. Placental lactogens induce serotonin biosynthesis in a subset of mouse beta cells during pregnancy. Diabetologia 2010; 53: 2589–2599.
Rodríguez Sánchez F, Casar J, del Aguila F, de Castro JM. Distribution of serotonin-immunoreactive cells in the mouse pancreas during development. Histol Histopathol 1990; 5; 259–266.