Jiun Yan LOH, Yau Yan LIM, Sharr Azni HARMIN, Adeline Su Yien TING

In vitro assessment on intestinal microflora from commonly farmed fishes for control of the fish pathogen Edwardsiella tarda

This study was aimed to evaluate the microflora isolated from the intestines of tilapia (Oreochromis niloticus), freshwater catfish (Clarias batrachus), snakehead (Ophicephalus striatus), snakeskin gourami (Trichogaster pectoralis), climbing perch (Anabas testudineus), and river catfish (Pangasius pangasius) for their potential probiotic development. Isolates were initially screened for their inhibitory effect against one of the most common fish pathogens, Edwardsiella tarda. Positive antagonists were subsequently tested for hemolytic activity, antibiotic susceptibility, and production of bacteriocin-like inhibitory substances (BLISs). Our results revealed 55 of the 405 isolates tested showed inhibitory effects towards the pathogen. Forty isolates were γ-hemolytic bacteria. Only 7 isolates were nonresistant to tested antibiotics. They were identified as Lactococcus lactis based on 16S rRNA characterization. Our BLIS data suggest that L. lactis subsp. lactis CF4MRS at the cell density of 106 cfu/mL could be effectively used to control E. tarda.

Anahtar Kelimeler:

Key words: Aquaculture, Edwardsiella tarda, lactic acid bacteria, Lactococcus lactis

In vitro assessment on intestinal microflora from commonly farmed fishes for control of the fish pathogen Edwardsiella tarda

Keywords:

Key words: Aquaculture, Edwardsiella tarda, lactic acid bacteria, Lactococcus lactis,

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Mainous ME, Smith SA, Kuhn DD. Effect of common aquaculture chemicals against Edwardsiella ictaluri and E. tarda. J Aquat Anim Health 2010; 22: 224–228.
Mohanty BR, Sahoo PK. Edwardsiellosis in fish: a brief review. J Biosci 2007; 32: 1331–1344.
Nucci C, Silveria WD, Correa SS. Nakazato G, Bando SY, Ribeiro MA, Castro AFP. Microbiological comparative study of isolates of Edwardsiella tarda isolated in different countries from fish and humans. Vet Microbiol 2002; 89: 29–39.
Srinivasa Rao PS, Yamada Y, Tan YP, Leung KY. Use of proteomics to identify novel virulence determinants that are required for Edwardsiella tarda pathogenesis. Mol Microbiol 2004; 53: 573–586.
Waltman WD, Shotts EB. Antimicrobial susceptibility of Edwardsiella tarda from the United States and Taiwan. Vet Microbiol 1986; 12: 277–282.
Clark RB, Lister PD, Janda JM. In vitro susceptibilities of Edwardsiella tarda to 22 antibiotics and antibiotic-betalactamase-inhibitor agents. Diagn Micr Infect Dis 1991; 14: 173–175.
Taoka K, Maeda H, Jo JY, Kim SM, Park SI, Yoshikawa T, Sakata T. Use of live and dead probiotic cells in tilapia Oreochromis niloticus. Fisheries Sci 2006; 72: 755–766.
Pintado J, Pérez-Lorenzo M, Luna-González A, Sotelo CG. Monitoring of the bioencapsulation of a probiotic Phaeobacter strain in the rotifer Brachionus plicatilis using denaturing gradient gel electrophoresis. Aquaculture 2010; 302: 182–194. Chang CI, Liu WY. An evaluation of two probiotic bacterial strains, Enterococcus faecium SF 68 and Bacilus toyoi, for reducing edwardsiellosis in cultured European eel, Anguilla anguilla L. J Fish Dis 2002; 25: 311–315.
Sahu MK, Swarnakumar NS, Sivakumar K, Thangaradjou T, Kannan L. Probiotics in aquaculture: importance and future perspectives. Indian J Microbiol 2008; 48: 299–308.
Ting ASY, Tan SH, Wai MK. Isolation and characterization of Actinobacteria with antibacterial activity from soil and rhizosphere soil. Aust J Basic Appl Sci 2009; 3: 4053–4059.
Nurhidayu A, Ina-Salwany MY, Mohd Daud H, Harmin SA. Isolation, screening and characterization of potential probiotics from farmed tiger grouper (Epinephelus fuscoguttatus). Afr J Microbiol Res 2012; 6: 1924–1933.
Bell SM, Pham JN, Newton PJ, Nguyen TT. A Manual for Medical and Veterinary Laboratories. 7th ed. Randwick, Australia: South Eastern Area Laboratory Services; 2013.
Cabello FC. Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environ Microbiol 2006; 8: 1137– 11
Cahill MM. Bacterial flora of fishes: a review. Microbial Ecol 1990; 19: 21–41.
Maslow JN, Dawson D, Carlin EA, Holland SM. Hemolysin as a virulence factor for systemic infection with isolates of Mycobacterium avium complex. J Clin Microbiol 1999; 37: 445–446.
Romero J, Feijoó CG, Navarrete P. Antibiotics in aquaculture– use, abuse and alternatives. In: ED Carvalho, G Silva David, RJ da Silva, editors. Health and Environment in Aquaculture. Rijeka, Croatia: InTech; 2012. pp. 159–198.
Akinbowale OL, Peng H, Barton MD. Antimicrobial resistance in bacteria isolated from aquaculture sources in Australia. J Appl Microbiol 2006; 100: 1103–1113.
Hansen GH, Olafsen JA. Bacterial interactions in early life stages of marine cold water fish. Microbial Ecol 1999; 38: 1–26. Foster JW, Hall MK. Adaptive acidification tolerance response of Salmonella typhimurium. J Bacteriol 1990; 172: 771–778.