Effects of addition of vitamin $B_{12}$ to the extender on post-thaw motility, acrosome morphology, and plasma membrane integrity in bull semen
Effects of addition of vitamin $B_{12}$ to the extender on post-thaw motility, acrosome morphology, and plasma membrane integrity in bull semen
To investigate the effects of supplementation of vitamin $B_{12}$ on bovine sperm post-thaw quality, vitamin $B_{12}$ was added at the concentrations of 1.25, 2.50, 3.75, and 5.00 mg/mL to bovine semen cryoprotective medium. The results indicated that the motility and VSL, VCL, STR, and VAP values of sperm supplemented with 2.50 mg/mL vitamin $B_{12}$ were significantly higher than those of other concentrations (P < 0.05). No significant difference was observed for LIN and ALH values and the percentage of grade a spermatozoa between the extenders containing 2.50 mg/mL and 3.75 mg/mL vitamin $B_{12}$ (P > 0.05). The percentages of acrosome-intact and plasma membrane-intact spermatozoa were significantly improved (P < 0.05) by supplementing with 2.50 mg/mL vitamin $B_{12}$ . With all parameters measured, the concentration of 2.50 mg/mL vitamin $B_{12}$ showed the best results on the quality of bovine semen in freezing-thawing. In conclusion, the addition of 2.50 mg/mL vitamin $B_{12}$ to the extender improved the bovine frozen semen quality.
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- 1. Juanchi, X., Albarran, G., Negron-Mendoza, A.: Radiolysis of cyanocobalamin (vitamin B12). Radiat. Phys. Chem., 2000; 57: 337-339.
- 2. Goodhope, C.D.: The treatment of oligospermia with stilbestrol and vitamin B. Fertil. Steril., 1961; 12: 469-473.
- 3. Tomaszewski, L., Zmudzka, B., Nadworny, J.: Seminal vitamin $B_{12}$ and sterility. Lancet, 1963; 1: 170.
- 4. Watanabe, T., Ohkawa, K., Kasai, S., Ebara, S., Nakano, Y., Watanabe, Y.: The effects of dietary vitamin B12-deficiency on sperm maturation in developing and growing male rats. Congenit Anom., 2003; 43: 57-64.
- 5. Ames, B.N.: Micronutrients prevent cancer and delay aging. Toxicol. Lett., 1998; 102-103: 5-18.
- 6. van Pelt, A.M., de Rooij, D.G.: Retinoic acid is able to reinitiate spermatogenesis in vitamin A-deficient rats and high replicate doses support the full development of spermatogenic cells. Endocrinology, 1991; 128: 697-704.
- 7. Ciereszko, A., Dabrowski, K.: Sperm quality and ascorbic acid concentration in rainbow trout semen are affected by dietary vitamin C: an across-season study. Biol. Reprod., 1995; 52: 982- 988.
- 8. Ha, F., Zhao, Y.Z.: Vitamin B complex as a complement in the thawing dilutions of the ram semen. China Herbivores, 2003; 23: 19-20. (article in Chinese with an abstract in English)
- 9. Cai, J.G., Sun, S.Q., Wang, L.G., Gu, H.J.: The effect of adding vitamin B12 in sperm diluter on quality of bull’s straw frozen sperm. J. Liaoning Agricult. Coll., 2004; 6: 10-11. (article in Chinese with an abstract in English)
- 10. Aboagla, E.M., Terada, T.: Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biol. Reprod., 2003; 69: 1245-1250.
- 11. Sharp, A.A., Witts, L.J.: Seminal vitamin B12 and sterility. Lancet, 1962; 1: 779.
- 12. Pardanani, D.S., Kher, Y.R., Mahendrakar, M.N., Kirtane, S.T., Jayatilak, P.G.: Effects of testosterone-vitamin $B_{12}$ (Aquaviron $B_{12}$ ) therapy in cases of oligospermia. Indian J. Surg., 1972; 34: 89-96.
- 13. Halim, A., Antoniou, D., Leedham, P.W., Blandy, J.P., Tresidder, G.C.: Investigation and treatment of the infertile male. Proc. R. Soc. Med., 1973; 66: 373-378.
- 14. Hirwe, R., Jathar, V.S., Desai, S., Satoskar, R.S.: Vitamin $B_{12}$ and potential fertility in male lactovegetarians. J. Biosoc. Sci., 1976; 8: 221-227.
- 15. Jeyendran, R.S., Van der Ven, H.H., Perez-Pelaez, M., Crabo, B.G., Zaneveld, L.J.D.: Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil., 1984; 70: 219-228.
- 16. Bergman, E.N., Roe, W.E., Kon, K.: Quantitative aspects of proprionate metabolism and gluconeogenesis in sheep. Am. J. Physiol., 1966; 211: 793-799.
- 17. Kennedy, D.G., Cannavan, A., Molloy, A., O’Harte, F., Taylor, S.M., Kennedy, S., Blanchflower, W.J.: Methylmalonyl-CoA mutase (EC 5.4.99.2) and methionine synthetase (EC 2.1.1.13) in the tissues of cobalt-vitamin $B_{12}$ deficient sheep. Br. J. Nutr., 1990; 64: 721-732.
- 18. Shangari, N., Bruce, W.R., Poon, R., O’Brien, P.J.: Toxicity of glyoxals-role of oxidative stress, metabolic detoxification and thiamine deficiency. Biochem. Soc. Trans., 2003; 31: 1390-1393.
- 19. Vézina, D., Mauffette, F., Roberts, K.D., Bleau, G.: Seleniumvitamin E supplementation in infertile men. Effects on semen parameters and micronutrient levels and distribution. Biol. Trace Elem. Res., 1996; 53: 65-83.
- 20. Alvarez, J.G., Storey, B.T.: Assessment of cell damage caused by spontaneous lipid peroxidation in rabbit spermatozoa. Biol. Reprod., 1984; 30: 323-331.